Effect of three types of thermal processing methods on the lipidomics profile of tilapia fillets by UPLC-Q-Extractive Orbitrap mass spectrometry.

Thermal processing methods have important effects on food lipids. In this work, ultra-high-performance liquid chromatography-Q-Extractive Orbitrap mass spectrometry and lipidsearch software were applied to analyze effect of three types of thermal processing methods on the lipidomics profile of tilapia fillets. A total 15 classes of compound lipids (Cer, DG, LPC, LPE, LPG, LPI, LPS, PC, PE, PG, PI, PS, SM, So, TG) were analyzed. In addition, free DHA, EPA, and ARA were also identified. Furthermore, statistical analyses of these lipids were performed based on MetaboAnalyst software. The results demonstrated three types of thermal processing methods had different effects on lipidomics profile differences of tilapia fillets. A total of eight lipid species variables (LPS, LPG, LPI, DG, LPC, TG, LPE, and Cer) and 137 individual lipids variables showed significant differences among raw, steamed, boiled, and roasted tilapia fillets. This work could provide useful information for aquatic product processing and lipidomics.

[1]  Sabine Sampels,et al.  The effects of processing technologies and preparation on the final quality of fish products , 2015 .

[2]  Bárbara Nieva-Echevarría,et al.  Changes provoked by boiling, steaming and sous-vide cooking in the lipid and volatile profile of European sea bass. , 2017, Food research international.

[3]  Gang Chen,et al.  Lipidomics profiling of goat milk, soymilk and bovine milk by UPLC-Q-Exactive Orbitrap Mass Spectrometry. , 2017, Food chemistry.

[4]  V. Gökmen,et al.  Future perspectives in Orbitrap™-high-resolution mass spectrometry in food analysis: a review , 2015, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[5]  Yifen Wang,et al.  Nanoimaging of food proteins by atomic force microscopy. Part II: Application for food proteins from different sources , 2019, Trends in Food Science & Technology.

[6]  Qiangqiang Li,et al.  UPLC-Q-Exactive Orbitrap/MS-Based Lipidomics Approach To Characterize Lipid Extracts from Bee Pollen and Their in Vitro Anti-Inflammatory Properties. , 2017, Journal of agricultural and food chemistry.

[7]  Galina A. Gubanenko,et al.  Effect of boiling and frying on the content of essential polyunsaturated fatty acids in muscle tissue of four fish species , 2007 .

[8]  David S. Wishart,et al.  MetaboAnalyst: a web server for metabolomic data analysis and interpretation , 2009, Nucleic Acids Res..

[9]  P. Kris-Etherton,et al.  Dietary reference intakes for DHA and EPA. , 2009, Prostaglandins, leukotrienes, and essential fatty acids.

[10]  Lipidomic analysis of plasma samples from women with polycystic ovary syndrome , 2014, Metabolomics.

[11]  Shanshan Zhang,et al.  Targeted lipidomics profiling of marine phospholipids from different resources by UPLC-Q-Exactive Orbitrap/MS approach. , 2018, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[12]  Zahra Momenzadeh,et al.  Effect of different cooking methods on vitamins, minerals and nutritional quality indices of orange-spotted grouper (Epinephelus coioides) , 2017, Journal of Food Measurement and Characterization.

[13]  A. Leaf,et al.  Essentiality of and Recommended Dietary Intakes for Omega-6 and Omega-3 Fatty Acids , 1999, Annals of Nutrition and Metabolism.

[14]  L. Mondello,et al.  Determination of phospholipids in milk samples by means of hydrophilic interaction liquid chromatography coupled to evaporative light scattering and mass spectrometry detection. , 2011, Journal of chromatography. A.

[15]  W. J. Dyer,et al.  A rapid method of total lipid extraction and purification. , 1959, Canadian journal of biochemistry and physiology.

[16]  David S. Wishart,et al.  MetaboAnalyst 4.0: towards more transparent and integrative metabolomics analysis , 2018, Nucleic Acids Res..

[17]  N. Noguchi,et al.  Actual ratios of triacylglycerol positional isomers consisting of saturated and highly unsaturated fatty acids in fishes and marine mammals. , 2011, Food chemistry.

[18]  I. Valterová,et al.  Effect of heat treatment on the n-3/n-6 ratio and content of polyunsaturated fatty acids in fish tissues. , 2015, Food chemistry.

[19]  C. Glass,et al.  A comprehensive classification system for lipids. , 2005, Journal of lipid research.

[20]  Qingbiao Zhao,et al.  Sensitive and rapid determination of glyphosate, glufosinate, bialaphos and metabolites by UPLC-MS/MS using a modified Quick Polar Pesticides Extraction method. , 2018, Forensic science international.

[21]  E. Fukusaki,et al.  Development of a lipid profiling system using reverse-phase liquid chromatography coupled to high-resolution mass spectrometry with rapid polarity switching and an automated lipid identification software. , 2013, Journal of chromatography. A.

[22]  Yiqun Huang,et al.  Dynamic Viscoelastic Properties of Tilapia (Oreochromis niloticus) Skin Gelatin , 2016 .

[23]  S. Quek,et al.  Effect of cooking method on the fatty acid profile of New Zealand King Salmon (Oncorhynchus tshawytscha). , 2010 .

[24]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[25]  Yingyun Qiao,et al.  Extraction and characterization of type I collagen from skin of tilapia (Oreochromis niloticus) and its potential application in biomedical scaffold material for tissue engineering , 2018, Process Biochemistry.

[26]  J. Namieśnik,et al.  Comprehensive methodology for Staphylococcus aureus lipidomics by liquid chromatography and quadrupole time-of-flight mass spectrometry. , 2014, Journal of chromatography. A.

[27]  Cuiping Shi,et al.  Nanoimaging of food proteins by atomic force microscopy. Part I: Components, imaging modes, observation ways, and research types , 2019, Trends in Food Science & Technology.