Evaluation of phenolic compounds in commercial fruit juices and fruit drinks.

The total phenolic content of 13 commercially available fruit juices and juice drinks, selected to represent the most popular juice flavors in the United Kingdom, were analyzed using the Folin-Ciocalteu assay. Individual phenolic compounds were identified and quantified using HPLC-PDA-MS2. The catechin content and degree of polymerization of proanthocyanidins were also analyzed. Purple grape juice contained the largest number of individual phenolic compounds and also the highest concentration of total phenolics. The main components were flavan-3-ols, anthocyanins, and hydroxycinnamates, which accounted for 93% of the total phenolic content. In contrast, white grape juice, which contained principally hydroxycinnamates, had the lowest total phenolic content. Antioxidant activity was measured using the ORAC and FRAP assays, and the data obtained were in broad agreement with total phenol content. In view of the recent findings of the Kame project indicating that long-term fruit juice consumption can provide protection against Alzheimer's disease (Dai et al. Am. J. Med. 2006, 379, 464-475), it is suggested that the protective effects may be enhanced by consumption of a combination of juices rich in phenolics and containing a diverse variety of individual phenolic compounds, namely, juices derived from purple grapes, grapefruit, cranberries, and apples.

[1]  A. Crozier,et al.  Flavonoid and chlorogenic acid profiles of English cider apples , 2007 .

[2]  J. Strain,et al.  Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. , 1999, Methods in enzymology.

[3]  A. Gescher,et al.  Relationship between mechanisms, bioavailibility, and preclinical chemopreventive efficacy of resveratrol: a conundrum. , 2003, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.

[4]  Nih State-of-the-Science Panel.  National Institutes of Health State-of-the-Science Conference Statement: Multivitamin/Mineral Supplements and Chronic Disease Prevention , 2006, Annals of Internal Medicine.

[5]  A. Lavy,et al.  Consumption of red wine with meals reduces the susceptibility of human plasma and low-density lipoprotein to lipid peroxidation. , 1995, The American journal of clinical nutrition.

[6]  M. Lean,et al.  Relationship among antioxidant activity, vasodilation capacity, and phenolic content of red wines. , 2000, Journal of agricultural and food chemistry.

[7]  C. Felgines,et al.  Anthocyanin metabolism in rats and their distribution to digestive area, kidney, and brain. , 2005, Journal of agricultural and food chemistry.

[8]  M. Carrier,et al.  Oenology: Red wine procyanidins and vascular health , 2006, Nature.

[9]  B. Shukitt-Hale,et al.  Anthocyanins in aged blueberry-fed rats are found centrally and may enhance memory , 2005, Nutritional neuroscience.

[10]  E. Feskens,et al.  Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study , 1993, The Lancet.

[11]  R. M. Alonso-Salces,et al.  Chemometric characterisation of Basque and French ciders according to their polyphenolic profiles , 2004, Analytical and bioanalytical chemistry.

[12]  F. Mattivi,et al.  Fast access of some grape pigments to the brain. , 2005, Journal of agricultural and food chemistry.

[13]  M R Cullen,et al.  Risk factors for lung cancer and for intervention effects in CARET, the Beta-Carotene and Retinol Efficacy Trial. , 1996, Journal of the National Cancer Institute.

[14]  R. Wrolstad,et al.  Phenolic Composition of Authentic Pineapple Juice , 2002 .

[15]  J. Brümmer.  Drinking your health? It's too early to say , 2003, Nature.

[16]  D. Fitzpatrick,et al.  Endothelium-dependent vasorelaxing activity of wine and other grape products. , 1993, The American journal of physiology.

[17]  C. Rice-Evans,et al.  Uptake and metabolism of epicatechin and its access to the brain after oral ingestion. , 2002, Free radical biology & medicine.

[18]  J. Vinson,et al.  Effect of green and black tea supplementation on lipids, lipid oxidation and fibrinogen in the hamster: mechanisms for the epidemiological benefits of tea drinking , 1998, FEBS letters.

[19]  C. Ruxton,et al.  Can pure fruit and vegetable juices protect against cancer and cardiovascular disease too? A review of the evidence , 2006, International journal of food sciences and nutrition.

[20]  Michael N. Clifford,et al.  Chlorogenic acids and other cinnamates – nature, occurrence and dietary burden , 1999 .

[21]  N. Vorsa,et al.  Inhibition of the adherence of P-fimbriated Escherichia coli to uroepithelial-cell surfaces by proanthocyanidin extracts from cranberries. , 1998, The New England journal of medicine.

[22]  S. Renaud,et al.  Wine, alcohol, platelets, and the French paradox for coronary heart disease , 1992, The Lancet.

[23]  Alan Crozier,et al.  Variations in the profile and content of anthocyanins in wines made from cabernet sauvignon and hybrid grapes. , 2002, Journal of agricultural and food chemistry.

[24]  A. Cochrane,et al.  FACTORS ASSOCIATED WITH CARDIAC MORTALITY IN DEVELOPED COUNTRIES WITH PARTICULAR REFERENCE TO THE CONSUMPTION OF WINE , 1979, The Lancet.

[25]  V. Schini-Kerth,et al.  Grape juice causes endothelium-dependent relaxation via a redox-sensitive Src- and Akt-dependent activation of eNOS. , 2007, Cardiovascular research.

[26]  D. B. Min,et al.  Bioactivity of Resveratrol , 2006 .

[27]  A. Bast,et al.  A quantum chemical explanation of the antioxidant activity of flavonoids. , 1996, Chemical research in toxicology.

[28]  P. Nichelli,et al.  Do flavan-3-ols from green tea reach the human brain? , 2006, Nutritional neuroscience.

[29]  T. Yokota,et al.  Secondary Metabolites in Fruits, Vegetables, Beverages and Other Plant‐based Dietary Components , 2007 .

[30]  A. Crozier,et al.  The effects of cranberry juice consumption on antioxidant status and biomarkers relating to heart disease and cancer in healthy human volunteers , 2006, European journal of nutrition.

[31]  E. Rimm,et al.  Review of moderate alcohol consumption and reduced risk of coronary heart disease: is the effect due to beer, wine, or spirits? , 1996, BMJ.

[32]  G. Gattuso,et al.  Flavonoid glycosides in bergamot juice (Citrus bergamia Risso). , 2006, Journal of agricultural and food chemistry.

[33]  B. Bartolomé,et al.  Simultaneous Determination of Nonanthocyanin Phenolic Compounds in Red Wines by HPLC-DAD/ESI-MS , 2005, American Journal of Enology and Viticulture.

[34]  Dejian Huang,et al.  High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. , 2002, Journal of agricultural and food chemistry.

[35]  Qi Dai,et al.  Fruit and vegetable juices and Alzheimer's disease: the Kame Project. , 2006, The American journal of medicine.

[36]  Haibo Wang,et al.  Characterization of anthocyanins in grape juices by ion trap liquid chromatography-mass spectrometry. , 2003, Journal of agricultural and food chemistry.

[37]  V. L. Singleton,et al.  Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents , 1965, American Journal of Enology and Viticulture.

[38]  F. Tomás-Barberán,et al.  Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. , 2000, Journal of agricultural and food chemistry.