Cu2CdSnS4 (CCTS) quaternary alloy nanostructure is an important material of enhancing the sensors effectiveness. CCTS nanostructures have been synthesized using ultrasonic and deposited on SiO2/Si substrate using sol-gel method and under annealing temperature; 400 oC. Structural optical, topographical and morphological properties of CCTS were explored using Ultraviolet-visible spectroscopy, X-ray diffraction (XRD), atomic force microscopy (AFM) and scanning electron microscopy (SEM), respectively. Interdigitated electrodes (IDEs) have been manufactured via deposited silver on CCTS/SiO2/Si substrate via a physical vapor deposition and hard mask. Electrical properties of fabricated IDEs are further evaluated as a function of voltage and frequency. The proposed IDEs can be used in subsequent studies to develop a biological sensor capable of detecting the DNA for many types of diseases in order to facilitate the combating and eradicating. The resulted values are in agreement with others.