Purification of a

Automated chemical synthesis of DNA has improved rapidly, with substantial gains made in the chemistry enabling routine coupling yields in excess of 99% and very reliable automation with each synthesis cycle of less than 3 min. The final oligonucleotide product obtained in the 20-30mer range is substantially pure with very low truncated sequences, thus requiring no further purification for most routine applications involving Polymerase Chain Reaction (PCR*) and DNA sequencing. Purification may be required for other applications, and is recommended for cloning, site directed mutagenesis, ligation etc.