Xylitol improves pancreatic islets morphology to ameliorate type 2 diabetes in rats: a dose response study.

UNLABELLED Xylitol has been reported as a potential antidiabetic sweetener in a number of recent studies; however, the most effective dietary dose and organ-specific effects are still unclear. Six-week-old male Sprague-Dawley rats were randomly divided into 5 groups: normal control (NC), diabetic control (DBC), diabetic xylitol 2.5% (DXL2.5), diabetic xylitol 5.0% (DXL5), and diabetic xylitol 10.0% (DXL10). Diabetes was induced only in the animals in DBC and DXL groups and considered diabetic when their nonfasting blood glucose level was >300 mg/dL. The DXL groups were fed with 2.5%, 5.0%, and 10% xylitol solution, whereas the NC and DBC groups were supplied with normal drinking water. After 4-wk intervention, body weight, food and fluid intake, blood glucose, serum fructosamine, liver glycogen, serum alanine transaminase, aspartate transaminase, lactate dehydrogenase, creatine kinase, uric acid, creatinine, and most serum lipids were significantly decreased, and serum insulin concentration, glucose tolerance ability, and pancreatic islets morphology were significantly improved in the DXL10 group compared to the DBC group. The data of this study suggest that 10% xylitol has the better antidiabetic effects compared to 2.5% and 5.0% and it can be used as an excellent antidiabetic sweetener and food supplement in antidiabetic foods. PRACTICAL APPLICATION Xylitol is widely used as a potential anticariogenic and sweetening agent in a number of oral care and food products when many of its health benefits are still unknown. Due to its similar sweetening power but lower calorific value (2.5 compared with 4 kcal) and lower glycemic index (13 compared with 65) compared to sucrose, recently it has been widely used as a sugar substitute particularly by overweight, obese, and diabetic patients in order to reduce their calorie intake from sucrose. However, the potential antidiabetic effects of xylitol have been discovered recently. The results of this study confirmed the effective dietary dose of xylitol for diabetics with some of the mechanisms of actions behind its antidiabetic effects.

[1]  J. Moon,et al.  Reducing Serum Uric Acid Attenuates TGF-β1-Induced Profibrogenic Progression in Type 2 Diabetic Nephropathy , 2013, Nephron Experimental Nephrology.

[2]  Mahmoodi Mehdi,et al.  Hepatoprotective effects of hydroalcoholic extract of Allium hirtifolium (Persian shallot) in diabetic rats , 2012, Journal of basic and clinical physiology and pharmacology.

[3]  M. Islam,et al.  Effects of Xylitol on Blood Glucose, Glucose Tolerance, Serum Insulin and Lipid Profile in a Type 2 Diabetes Model of Rats , 2012, Annals of Nutrition and Metabolism.

[4]  M. Hu,et al.  Xylitol prevents NEFA-induced insulin resistance in rats , 2012, Diabetologia.

[5]  S. Kahn,et al.  Risk factors for type 2 diabetes: Lessons learned from Japanese Americans in Seattle , 2012, Journal of diabetes investigation.

[6]  K. Stanhope.  Role of fructose-containing sugars in the epidemics of obesity and metabolic syndrome. , 2012, Annual review of medicine.

[7]  X. Qin.  What made Canada become a country with the highest incidence of inflammatory bowel disease: could sucralose be the culprit? , 2011, Canadian journal of gastroenterology = Journal canadien de gastroenterologie.

[8]  B. Jyothirmayi,et al.  Insulin resistance and alanine amino transaminase (ALT) levels in first degree relatives of type 2 diabetes mellitus. , 2011, Diabetes & metabolic syndrome.

[9]  E. Takeda,et al.  Effects of xylitol on metabolic parameters and visceral fat accumulation , 2011, Journal of clinical biochemistry and nutrition.

[10]  M. Islam.  Effects of xylitol as a sugar substitute on diabetes-related parameters in nondiabetic rats. , 2011, Journal of medicinal food.

[11]  J. Maillefert,et al.  Aspartame-induced fibromyalgia, an unusual but curable cause of chronic pain. , 2010, Clinical and experimental rheumatology.

[12]  Gary M Williams,et al.  Expert panel report on a study of Splenda in male rats. , 2009, Regulatory toxicology and pharmacology : RTP.

[13]  R. McLendon,et al.  Splenda Alters Gut Microflora and Increases Intestinal P-Glycoprotein and Cytochrome P-450 in Male Rats , 2008, Journal of toxicology and environmental health. Part A.

[14]  J. Boullata,et al.  The potential toxicity of artificial sweeteners. , 2008, AAOHN journal : official journal of the American Association of Occupational Health Nurses.

[15]  D. Davis,et al.  Aspartame and Incidence of Brain Malignancies , 2008, Cancer Epidemiology Biomarkers & Prevention.

[16]  Sarah A Stechschulte,et al.  Formaldehyde, aspartame, and migraines: a possible connection. , 2008, Dermatitis : contact, atopic, occupational, drug.

[17]  A. Mukherjee,et al.  Genotoxicity Testing of Low-Calorie Sweeteners: Aspartame, Acesulfame-K, and Saccharin , 2008 .

[18]  V. Lee Grotz.  Sucralose and Migraine , 2007, Headache.

[19]  J. Huff,et al.  Aspartame Bioassay Findings Portend Human Cancer Hazards , 2007, International journal of occupational and environmental health.

[20]  A. Hirsch.  Migraine Triggered by Sucralose—A Case Report , 2007, Headache.

[21]  F. Bornet,et al.  Gastrointestinal tolerance of erythritol and xylitol ingested in a liquid , 2007, European Journal of Clinical Nutrition.

[22]  E. G. Abegaz.  Aspartame Not Linked to Cancer , 2007, Environmental health perspectives.

[23]  W. Zang,et al.  [A method to produce the animal model of diabetic cardiomyopathy]. , 2006, Wei sheng yan jiu = Journal of hygiene research.

[24]  Rajendrakumar M. Patel,et al.  Popular Sweetner Sucralose as a Migraine Trigger , 2006, Headache.

[25]  M. Bigal,et al.  Migraine Triggered by Sucralose—A Case Report , 2006, Headache.

[26]  E. Millstone,et al.  Carcinogenicity of Saccharin in Laboratory Animals and Humans: Letter to Dr. Harry Conacher of Health Canada , 2002, International journal of occupational and environmental health.

[27]  E. Kolehmainen,et al.  Effects of dietary xylitol on collagen content and glycosylation in healthy and diabetic rats. , 2000, Life sciences.

[28]  E L Melanson,et al.  Overview of the determinants of overweight and obesity: current evidence and research issues. , 1999, Medicine and science in sports and exercise.

[29]  M. Knuuttila,et al.  Dietary xylitol supplementation prevents osteoporotic changes in streptozotocin-diabetic rats. , 1998, Metabolism: clinical and experimental.

[30]  J. Wehner,et al.  Effects of sodium ascorbate, sodium saccharin and ammonium chloride on the male rat urinary bladder. , 1995, Carcinogenesis.

[31]  S. Salminen,et al.  Xylitol vs glucose: effect on the rate of gastric emptying and motilin, insulin, and gastric inhibitory polypeptide release. , 1989, The American journal of clinical nutrition.

[32]  M. Hämäläinen.  Organic aciduria in rats fed high amounts of xylitol or sorbitol. , 1987, Toxicology and applied pharmacology.

[33]  J. Morley,et al.  Effects of xylitol on gastric emptying and food intake. , 1987, The American journal of clinical nutrition.

[34]  G. Zbinden,et al.  Early biochemical and morphological changes of the rat adrenal medulla induced by xylitol , 1985, Archives of Toxicology.

[35]  S. Salminen,et al.  The effects of xylitol on gastric emptying and secretion of gastric inhibitory polypeptide in the rat. , 1984, The Journal of nutrition.

[36]  S. Salminen,et al.  The effects of xylitol on the secretion of insulin and gastric inhibitory polypeptide in man and rats , 1982, Diabetologia.

[37]  J. Beyer,et al.  The effects of equal caloric amounts of xylitol, sucrose and starch on insulin requirements and blood glucose levels in insulin-dependent diabetics , 1981, Diabetologia.

[38]  M. Wekell,et al.  Incidence of increased numbers of Clostridium perfringens in the intestinal tract of rats fed xylitol. , 1980, The Journal of nutrition.

[39]  K. Kosaka,et al.  Comparison of the Early Time Courses of the Release of Insulin That Follow Injections of Tetragastrin, Tolbutamide, Xylitol, and Glucose into the Pancreatic Artery of Dogs , 1978, Diabetes.

[40]  T. Asano,et al.  Xylitol, a Partial Homologue of α-D-Glucopyranose: Potent Stimulator of Insulin Release in Dogs , 1977 .

[41]  A. Taylor,et al.  Determination of glycogen in small tissue samples. , 1970, Journal of applied physiology.

[42]  E. Carraro,et al.  Formaldehyde adduct to human serum albumin with reference to aspartame intake. , 2008, Environmental toxicology and pharmacology.

[43]  S. Cohen.  What's the truth about the health risks of sugar substitutes such as saccharin and aspartame? , 2001, Health news.

[44]  Q. Khalid,et al.  Studies on xylitol-induced insulin secretion in vitro. , 1984, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.