Prognostic value of MIB‐1 in advanced ovarian carcinoma as determined using automated immunohistochemistry and quantitative image analysis

The monoclonal antibody MIB‐1 is an immunohistochemical marker reacting most strongly with cells in late S phase, G2, and M portions of the cell cycle. This antibody, reactive in formalin‐fixed, paraffin‐embedded tissue, allows the quantitation of a proliferation index (PI) in both current clinical cases and archival material using a computerized image analyzer (CIA).

[1]  P. Humphrey,et al.  Mutation and overexpression of p53 in early-stage epithelial ovarian cancer. , 1993, Obstetrics and gynecology.

[2]  M. Volm,et al.  Flow-cytometric prognostic factors for the survival of patients with ovarian carcinoma: a 5-year follow-up study. , 1989, Gynecologic oncology.

[3]  J. Bacus,et al.  The evaluation of estrogen receptor in primary breast carcinoma by computer-assisted image analysis. , 1988, American journal of clinical pathology.

[4]  S. Tseng,et al.  Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies , 1982, Cell.

[5]  Ron Brookmeyer,et al.  A Confidence Interval for the Median Survival Time , 1982 .

[6]  R. Bast,et al.  Determination of proliferation index in advanced ovarian cancer using quantitative image analysis. , 1993, American journal of clinical pathology.

[7]  J. Meyer,et al.  Thymidine labeling index, flow cytometric S-phase measurement, and DNA index in human tumors. Comparisons and correlations. , 1988, American journal of clinical pathology.

[8]  R. Dodge,et al.  Determination of Proliferation Index By MIB‐1 Immunostaining in Early Stage Breast Cancer Using Quantitative Image Analysis , 1995 .

[9]  E. Kaplan,et al.  Nonparametric Estimation from Incomplete Observations , 1958 .

[10]  N. Ragni,et al.  Cell kinetics in ovarian cancer. Relationship to clinicopathologic features, responsiveness to chemotherapy, and survival , 1989, Cancer.

[11]  G. Fleuren,et al.  Tumor ploidy as a major prognostic factor in advanced ovarian cancer , 1987, Cancer.

[12]  T. Sebo,et al.  Proliferative activity in non-Hodgkin's lymphomas. A comparison of the bromodeoxyuridine labeling index with PCNA immunostaining and quantitative image analysis. , 1993, American journal of clinical pathology.

[13]  Johannes Gerdes,et al.  Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation , 1983, International journal of cancer.

[14]  Aimo Ojala.  Studies on Bilirubin in Amniotic Fluid: With Special Reference to Liver Function Tests , 1971, Acta obstetricia et gynecologica Scandinavica. Supplement.

[15]  M. Kubbutat,et al.  Cell proliferation-associated nuclear antigen defined by antibody Ki-67: a new kind of cell cycle-maintaining proteins. , 1995, Archivum immunologiae et therapiae experimentalis.

[16]  M. Kubbutat,et al.  Comparison of monoclonal antibodies PC 10 and MIB 1 on microwave‐processed paraffin sections , 1994 .

[17]  A. deFazio,et al.  A simple and rapid method for the identification of cycling cells in freshly excised tumours. , 1985, Cytobios.

[18]  L. Layfield,et al.  Proliferative index determination in prostatic carcinoma tissue: is there any additional prognostic value greater than that of Gleason score, ploidy and pathological stage? , 1997, The Journal of urology.

[19]  R. Bast,et al.  Determination of proliferation index with MIB-1 in advanced ovarian cancer using quantitative image analysis. , 1994, American journal of clinical pathology.

[20]  J. H. Taylor,et al.  THE ORGANIZATION AND DUPLICATION OF CHROMOSOMES AS REVEALED BY AUTORADIOGRAPHIC STUDIES USING TRITIUM-LABELED THYMIDINEE. , 1957, Proceedings of the National Academy of Sciences of the United States of America.

[21]  G M Clark,et al.  Prediction of relapse or survival in patients with node-negative breast cancer by DNA flow cytometry. , 1989, The New England journal of medicine.

[22]  M. Tattersall,et al.  Immunohistochemical determination of tumour growth fraction in human ovarian carcinoma , 1989, British journal of obstetrics and gynaecology.

[23]  M. Barbareschi,et al.  Quantitative growth fraction evaluation with MIB1 and Ki67 antibodies in breast carcinomas. , 1994, American journal of clinical pathology.

[24]  A. Howard,et al.  Synthesis of Desoxyribonucleic Acid in Normal and Irradiated Cells and Its Relation to Chromosome Breakage , 1986 .

[25]  O. Kallioniemi,et al.  Steroid receptors and Ki‐67 reactivity in ovarian cancer and in normal ovary: Correlation with dna flow cytometry, biochemical receptor assay, and patient survival , 1990, The Journal of pathology.

[26]  R. Galen,et al.  Beyond Normality: The Predictive Value and E ciency of Medical Diagnoses , 1975 .

[27]  R. Bast,et al.  Ploidy analysis of epithelial ovarian cancers using image cytometry. , 1992, Gynecologic oncology.