Development and validation of a rapid test system for detection of pork meat and collagen residues.

Mislabeling, contamination, and economic adulteration of meat products with undeclared pork tissues are illegal under regulations promulgated by numerous regulatory agencies. Nonetheless, analysis of the European meat industry has revealed pervasive meat adulteration, necessitating more extensive application of meat authentication testing. As existing methods for meat speciation require specialized equipment and/or training, we developed a detection system based on a lateral flow device (LFD) assay format capable of rapidly (~35min) identifying porcine residues derived from raw meat, cooked meat, and gelatin down to 0.01%, 1.0%, and 2.5% contamination, respectively. Specificity analysis revealed no cross-reactivity with meat derived from chicken, turkey, horse, beef, lamb, or goat. Comparison with a commercial ELISA kit and PCR method revealed similar if not improved sensitivity, with the added feature that the LFD-based system required considerably less time to perform. Accordingly, this test system should aid the food industry and food control authorities in monitoring for adulteration with pork.

[1]  F. Robert-Gangneux,et al.  Epidemiology of and Diagnostic Strategies for Toxoplasmosis , 2012, Clinical Microbiology Reviews.

[2]  S Muroya,et al.  A quick and simple method for the identification of meat species and meat products by PCR assay. , 1999, Meat science.

[3]  Md. Eaqub Ali,et al.  A lab-on-a-chip-based multiplex platform to detect potential fraud of introducing pig, dog, cat, rat and monkey meat into the food chain , 2015, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[4]  F. Toldrá,et al.  PCR-based fingerprinting techniques for rapid detection of animal species in meat products. , 2004, Meat science.

[5]  D. Cawthorn,et al.  A high incidence of species substitution and mislabelling detected in meat products sold in South Africa , 2013 .

[6]  Morgan R Alexander,et al.  Rapid detection of peptide markers for authentication purposes in raw and cooked meat using ambient liquid extraction surface analysis mass spectrometry. , 2014, Analytical chemistry.

[7]  Y. Man,et al.  Detection of pig derivatives in food products for halal authentication by polymerase chain reaction –restriction fragment length polymorphism , 2007 .

[8]  I. Martinez,et al.  Species identification in meat products by RAPD analysis , 1998 .

[9]  Morgan R Alexander,et al.  Authentication of processed meat products by peptidomic analysis using rapid ambient mass spectrometry. , 2015, Food chemistry.

[10]  A. Moussavi,et al.  Fraud identification in industrial meat products by multiplex PCR assay. , 2009 .

[11]  R. Köppel,et al.  Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, horse and sheep , 2008 .

[12]  F. Simoons,et al.  Traditional Use and Avoidance of Foods of Animal Origin: A Culture Historical View , 1978 .

[13]  T. Matsunaga,et al.  Effects of Processing Conditions on Species Identification of Meat Products. , 1999 .

[14]  Lihua Liu,et al.  Sensitive Monoclonal Antibody-based Sandwich ELISA for the Detection of Porcine Skeletal Muscle in Meat and Feed Products , 2006 .

[15]  A. Parente,et al.  Myoglobin as marker in meat adulteration: a UPLC method for determining the presence of pork meat in raw beef burger. , 2013, Food chemistry.

[16]  A. S. Rohman,et al.  Analysis of pork adulteration in beef meatball using Fourier transform infrared (FTIR) spectroscopy. , 2011, Meat science.

[17]  S. Ahn,et al.  Controlled Synthesis of Icosahedral Gold Nanoparticles and Their Surface-Enhanced Raman Scattering Property , 2007 .

[18]  J. Regenstein,et al.  The Kosher and Halal Food Laws. , 2003, Comprehensive reviews in food science and food safety.

[19]  A. Piestrzynska-Kajtoch,et al.  The species identification of bovine, porcine, ovine and chicken components in animal meals, feeds and their ingredients, based on COX I analysis and ribosomal DNA sequences , 2013 .

[20]  H. Humpf,et al.  New sensitive high-performance liquid chromatography-tandem mass spectrometry method for the detection of horse and pork in halal beef. , 2013, Journal of agricultural and food chemistry.