Variations of Leek yellow stripe virus Concentration in Garlic and Its Incidence in Argentina.

The purpose of this work was to determine variations in titer of Leek yellow stripe virus (LYSV) throughout the crop cycle and bulb storage, and to evaluate the incidence of infected plants in the main garlic-production regions of Argentina. One hundred plants with LYSV from each of five cultivars were analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) in six different vegetative stages in White- and Red-type garlic cultivars and seven stages in cv. Rosado Paraguayo, throughout the year. In two White-type garlic cultivars, LYSV showed peaks of viral concentration in May, at the beginning of the crop cycle, and in November, just before harvest. In two Red-type garlic selections, an increase was detected in November (period of bulbing). The highest virus titers for these four garlic cultivars were detected in devernalized clove. In Rosado Paraguayo, the peak virus concentration occurred in September prior to harvesting. In a survey at 14 different localities in Argentina, 3,066 random samples were analyzed. LYSV was found in 80 to 98% of the plants from all regions, except in Santa Cruz, where 34% of plants were infected. The importance of this study is that it allows us to recommend the most suitable moment of the year to make the analysis with DAS-ELISA.

[1]  S. Nome,et al.  Fluctuations of Prunus Necrotic Ringspot Virus (PNRSV) at Various Phenological Stages in Peach Cultivars. , 1999, Plant disease.

[2]  H. Lot,et al.  Effects of Onion Yellow Dwarf and Leek Yellow Stripe Viruses on Symptomatology and Yield Loss of Three French Garlic Cultivars. , 1998, Plant disease.

[3]  V. Conci,et al.  Effect of different viruses on yield of garlic (Allium sativum) 'Rosado Paraguayo'. , 1998 .

[4]  K. Natsuaki,et al.  Nucleotide sequence analysis of virus isolates indicates the presence of three potyvirus species in Allium plants , 1998, Archives of Virology.

[5]  J. S. Lee,et al.  Identification of one of the major viruses infecting garlic plants, garlic virus X. , 1997, Molecules and cells.

[6]  P. Rabinowicz,et al.  Immunological Detection of a GarV-Type Virus in Argentine Garlic Cultivars. , 1997, Plant disease.

[7]  V. Conci.  An overview of Allium viruses in Argentina. , 1997 .

[8]  T. Tsuneyoshi,et al.  Differentiation among garlic viruses in mixed infections based on RT-PCR procedures and direct tissue blotting immunoassays , 1996 .

[9]  H. Vetten,et al.  IDENTIFICATION, PARTIAL CHARACTERIZATION, AND DISTRIBUTION OF VIRUSES INFECTING ALLIUM CROPS IN SOUTH AND SOUTHEAST ASIA , 1994 .

[10]  R. Salomon,et al.  Serological detection of onion yellow dwarf virus in garlic. , 1994 .

[11]  V. Conci,et al.  Virus Free Garlic (Allium Sativum L.) Plants Obtained by Thermotherapy and Meristem Tip Culture , 1991 .

[12]  D. Walkey,et al.  Agronomic evaluation of virus-free and virus-infected garlic (Allium sativum L.) , 1989 .

[13]  C. W. Kuhn,et al.  Nature of resistance in soybean to cowpea chlorotic mottle virus , 1985 .

[14]  B. Young,et al.  Leek yellow stripe virus in New Zealand , 1980 .

[15]  G. Mink.  Identification of rugose mosaic-diseased cherry trees by enzyme-linked immunosorbent assay. , 1980 .

[16]  A. N. Adams,et al.  Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. , 1977, The Journal of general virology.

[17]  M. Verhoyen,et al.  Chlorotic streak of leeks. I. Identification of the causal agent. , 1973 .

[18]  C. E. Rooyen,et al.  Virus Diseases of Man , 1949, Pediatrics.

[19]  Wilson Smith.  Virus Diseases , 1943, Nature.