Mechanical stability of membrane nanotubular protrusions influenced by attachment of flexible rod-like proteins.

It is indicated that nonhomogeneous lateral distribution of membrane attached and flexible rod-like proteins (MRPs) may stabilize nanotubular membrane protrusions. We have shown that curvature induced accumulation of MRPs in the nanotubular membrane protrusion and the corresponding reduction of the membrane free energy are possible if the decrease of the deviatoric free energy of MRPs in the nanotubular protrusions is large enough to overcome the increase of the free energy due to decrease of configurational entropy in the process of lateral sorting of MRPs. The decrease of isotropic curvature energy of MRPs in the region of membrane protrusion is usually not sufficient for substantial MRPs sorting and consequent stabilization of the nanotubular membrane protrusions.

[1]  Khashayar Farsad,et al.  Mechanisms of membrane deformation. , 2003, Current opinion in cell biology.

[2]  R. Ravelli,et al.  Adhesion mechanism of human β2‐glycoprotein I to phospholipids based on its crystal structure , 1999, The EMBO journal.

[3]  M. Hammel,et al.  Solution Structure of Human and Bovine β2-Glycoprotein I Revealed by Small-angle X-ray Scattering , 2002 .

[4]  A. Iglič,et al.  Cylindrical shapes of closed lipid bilayer structures correspond to an extreme area difference between the two monolayers of the bilayer. , 1999, Journal of biomechanics.

[5]  G. Nemerow,et al.  Hydrodynamic, electron microscopic, and ligand-binding analysis of the Epstein-Barr virus/C3dg receptor (CR2). , 1989, The Journal of biological chemistry.

[6]  G. Willems,et al.  Role of divalency in the high-affinity binding of anticardiolipin antibody-beta 2-glycoprotein I complexes to lipid membranes. , 1996, Biochemistry.

[7]  Tomaz Slivnik,et al.  Elastic properties of biological membranes influenced by attached proteins. , 2007, Journal of biomechanics.

[8]  Manfred Kriechbaum,et al.  Solution structure of human and bovine beta(2)-glycoprotein I revealed by small-angle X-ray scattering. , 2002, Journal of molecular biology.

[9]  A. Petrov,et al.  MEMBRANES AND LYOTROPIC PHASESON SOME PROBLEMS IN THE THEORY OF ELASTIC AND FLEXOELECTRIC EFFECTS IN BILAYER LIPID MEMBRANES AND BIOMEMBRANES , 1976 .

[10]  Harold P. Erickson,et al.  Reconstitution of Contractile FtsZ Rings in Liposomes , 2008, Science.

[11]  H. Hägerstrand,et al.  Microtubes and nanotubes of a phospholipid bilayer membrane , 2002 .

[12]  P. Devaux,et al.  Shape change and physical properties of giant phospholipid vesicles prepared in the presence of an AC electric field. , 1996, Biophysical journal.

[13]  Peter Hinterdorfer,et al.  Membrane binding of beta2-glycoprotein I can be described by a two-state reaction model: an atomic force microscopy and surface plasmon resonance study. , 2005, The Biochemical journal.

[14]  A. Callan-Jones,et al.  Curvature-driven lipid sorting needs proximity to a demixing point and is aided by proteins , 2009, Proceedings of the National Academy of Sciences.

[15]  S. Timoshenko,et al.  Theory of elasticity , 1975 .

[16]  Kendall Powell.  Cell biology: Ahead of the curve , 2009, Nature.

[17]  Veronika Kralj-Iglic,et al.  Curvature-induced accumulation of anisotropic membrane components and raft formation in cylindrical membrane protrusions. , 2006, Journal of theoretical biology.

[18]  Roland P. May,et al.  Modular Structure of Solubilized Human Apolipoprotein B-100 , 2006, Journal of Biological Chemistry.

[19]  M. Angelova,et al.  Preparation of giant vesicles by external AC electric fields. Kinetics and applications , 1992 .

[20]  A. Schroit,et al.  Characterization of phosphatidylserine-dependent beta2-glycoprotein I macrophage interactions. Implications for apoptotic cell clearance by phagocytes. , 1998, The Journal of biological chemistry.

[21]  R Skalak,et al.  Mechanics and thermodynamics of biomembranes: part 1. , 1979, CRC critical reviews in bioengineering.

[22]  A. Tian,et al.  Sorting of lipids and proteins in membrane curvature gradients. , 2009, Biophysical journal.

[23]  Veronika Kralj-Iglič,et al.  Amphiphile-induced tubular budding of the bilayer membrane , 2005, European Biophysics Journal.

[24]  Roie Shlomovitz,et al.  Physical model of contractile ring initiation in dividing cells. , 2008, Biophysical journal.

[25]  W. Helfrich.  Blocked Lipid Exchange in Bilayers and its Possible Influence on the Shape of Vesicles , 1974, Zeitschrift fur Naturforschung. Section C, Biosciences.

[26]  Erich Sackmann,et al.  Bending elastic moduli of lipid bilayers : modulation by solutes , 1990 .

[27]  R. Discipio.  Ultrastructures and interactions of complement factors H and I. , 1992, Journal of immunology.

[28]  Michael M. Kozlov,et al.  How proteins produce cellular membrane curvature , 2006, Nature Reviews Molecular Cell Biology.

[29]  Soichi Takeda,et al.  Endophilin BAR domain drives membrane curvature by two newly identified structure‐based mechanisms , 2006, The EMBO journal.

[30]  R. Nossal.  Energetics of Clathrin Basket Assembly , 2001, Traffic.

[31]  Veronika Kralj-Iglič,et al.  Quadrupolar ordering of phospholipid molecules in narrow necks of phospholipid vesicles , 2006 .

[32]  B. Peter,et al.  BAR Domains as Sensors of Membrane Curvature: The Amphiphysin BAR Structure , 2004, Science.