IDENTIFICATION OF INTESTINAL MICROSPORIDIA BY TRICHROME STAINING AND CALCOFLUOR WHITE METHODS AMONG KIDNEY- TRANSPLANTED PATIENTS IN HAMADAN

Background and Objective : Microsporidia is an obligatory intracellular parasite known as an opportunistic infection in immunocompromised patients. Its laboratory diagnosis is relatively difficult and modified trichrome staining is a standard diagnostic method for detection of Microsporidia. The aim of present study was to identify intestinal Microsporidia in kidney- transplanted patients, using modified Trichrome Staining and Calcofluor White Methods. Material and Methods : In 2012, 180 stool specimens were taken from kidney transplanted- patients given immunosuppressive drugs in Hamadan, Iran. To identify Microsporidia, we use modified Trichrome Staining and Calcofluor White Methods. Results : The mean duration of kidney transplant and immunosuppressive drug using was 5.5 Years. Only one female patient was positive for Microsporidia. Conclusions: Owing to low frequency of this opportunistic infection among kidney transplanted patients, we can conclude that their hygienic conditions are good enough and they are not exposed with the parasites.

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