Effects of dietary N-carbamylglutamate on rumen fermentation parameters, and bacterial community diversity of Holstein dairy cows in Tibet

Introduction The Tibetan Plateau is characterized by low temperature and hypoxia. N-carbamylglutamic acid (NCG) can increase blood oxygen saturation, and have the potential to be used to prevent the high-altitude hypoxia stress state of cows. However, its beneficial effect on the rumen microbiota of Holstein dairy cows remains unclear. Methods Hence, the experiments 12 multiparous (parity ranged from 2 to 7) Holstein dairy cows (413.0 ± 42 kg) were randomly assigned to 2 treatments with 6 replicates in each treatment: basal diet (CON, control group) and basal diet plus 20 g/d/cow of NCG (NCG, experiment group), respectively. To study the effects of dietary NCG supplementation on rumen microbiota of Holstein dairy cows in Tibet. The experiment lasted for 45 days, with 15 days of pre-feeding and 30 days of formal trail period. Results The results showed that ruminal NH3-N concentration in NCG group was lower (p < 0.05) than that in the CON group, while molar proportion of acetic acid and total volatile fatty acid (VFA) concentration were increased (p < 0.05) with the addition of NCG. Microbial diversity increased (p < 0.05) in NCG group, with Bacteroidetes, Firmicutes, and Patescibacteria as the most abundant phyla. The KEGG pathway analysis showed that the potential function of ruminal bacteria was mainly enriched in metabolism (carbohydrates, amino acids, lipids, energy, and nucleotides) and genetic information processing (replication, repair, and translation). Conclusion In conclusion, NCG can improve rumen nitrogen utilization, total VFA and acetic acid production, and increase rumen microbial diversity, all of which could make the introduced Holstein dairy cows to better adapt to the harsh environment in Tibet and improve their production performance.

[1]  Xiukun Wu,et al.  Comparison of rumen bacterial communities between yaks (Bos grunniens) and Qaidam cattle (Bos taurus) fed a low protein diet with different energy levels , 2022, Frontiers in Microbiology.

[2]  F. Yan,et al.  N-Carbamoylglutamate Supplementation on the Digestibility, Rumen Fermentation, Milk Quality, Antioxidant Parameters, and Metabolites of Jersey Cattle in High-Altitude Areas , 2022, Frontiers in Veterinary Science.

[3]  R. Dewhurst,et al.  Review: Markers and proxies to monitor ruminal function and feed efficiency in young ruminants. , 2021, Animal : an international journal of animal bioscience.

[4]  G. Suen,et al.  Assessing the relationship between the rumen microbiota and feed efficiency in Nellore steers , 2021, Journal of Animal Science and Biotechnology.

[5]  F. Gu,et al.  Dietary supplementation with N-carbamoylglutamate initiated from the prepartum stage improves lactation performance of postpartum dairy cows , 2020, Animal nutrition.

[6]  F. Hou,et al.  Altitude influences microbial diversity and herbage fermentation in the rumen of yaks , 2020, BMC microbiology.

[7]  Huansheng Yang,et al.  Effects of dietary energy levels on rumen fermentation, microbiota, and gastrointestinal morphology in growing ewes , 2020, Food science & nutrition.

[8]  Zhang Wenxue,et al.  Effects of garlic addition on bacterial communities and the conversions of nitrate and nitrite in a simulated pickle fermentation system , 2020 .

[9]  Gavin M Douglas,et al.  PICRUSt2 for prediction of metagenome functions , 2020, Nature Biotechnology.

[10]  Jianguo Li,et al.  Effects of dietary N-carbamylglutamate supplementation on milk production performance, nutrient digestibility and blood metabolomics of lactating Holstein cows under heat stress , 2020, Animal Feed Science and Technology.

[11]  A. Nasir,et al.  Bacterial Origin and Reductive Evolution of the CPR Group. , 2020, Genome biology and evolution.

[12]  F. Rodríguez-Valera,et al.  Microbiome of the deep Lake Baikal, a unique oxic bathypelagic habitat , 2019, Limnology and Oceanography.

[13]  Zhengcheng Zeng,et al.  Determination of N-Carbamylglutamate in Feeds and Animal Products by High Performance Liquid Chromatography Tandem Mass Spectrometry , 2019, Molecules.

[14]  T. Yan,et al.  Rumen bacterial diversity of Tibetan sheep (Ovis aries) associated with different forage types on the Qinghai-Tibetan Plateau. , 2019, Canadian journal of microbiology.

[15]  K. Totsche,et al.  Predominance of Cand. Patescibacteria in Groundwater Is Caused by Their Preferential Mobilization From Soils and Flourishing Under Oligotrophic Conditions , 2019, Front. Microbiol..

[16]  J. Jiao,et al.  Integrative plasma proteomic and microRNA analysis of Jersey cattle in response to high-altitude hypoxia. , 2019, Journal of dairy science.

[17]  Yunqi Xiao,et al.  Effects of dietary N‐carbamylglutamate supplementation on growth performance, tissue development and blood parameters of yellow‐feather broilers , 2019, Poultry science.

[18]  Zhisheng Wang,et al.  Bacterial Community Diversity Associated With Different Utilization Efficiencies of Nitrogen in the Gastrointestinal Tract of Goats , 2019, Front. Microbiol..

[19]  J. Loor,et al.  Jugular arginine supplementation increases lactation performance and nitrogen utilization efficiency in lactating dairy cows , 2019, Journal of animal science and biotechnology.

[20]  J. Loor,et al.  Rapid Communication: Jugular infusion of arginine has a positive effect on antioxidant mechanisms in lactating dairy cows challenged intravenously with lipopolysaccharide. , 2018, Journal of animal science.

[21]  J. Loor,et al.  Effects of Dietary l-Arginine and N-Carbamylglutamate Supplementation on Intestinal Integrity, Immune Function, and Oxidative Status in Intrauterine-Growth-Retarded Suckling Lambs. , 2018, Journal of agricultural and food chemistry.

[22]  Alexander J. Probst,et al.  Differential depth distribution of microbial function and putative symbionts through sediment-hosted aquifers in the deep terrestrial subsurface , 2018, Nature Microbiology.

[23]  H. Nie,et al.  Metabolomic profiling in umbilical venous plasma reveals effects of dietary rumen‐protected arginine or N‐carbamylglutamate supplementation in nutrient‐restricted Hu sheep during pregnancy , 2017, Reproduction in domestic animals = Zuchthygiene.

[24]  Liuqin He,et al.  Effects of Dietary α - , 2017 .

[25]  Meng Wang.  1500 Effects of arginine infusion through jugular vein on the milk performance and casein synthesis in midlactation cows. , 2016 .

[26]  F. Zhao,et al.  Convergent Evolution of Rumen Microbiomes in High-Altitude Mammals , 2016, Current Biology.

[27]  J. Jiao,et al.  Age and feeding system (supplemental feeding versus grazing) modulates colonic bacterial succession and host mucosal immune maturation in goats. , 2016, Journal of animal science.

[28]  J. Doré,et al.  Gut microbiota richness promotes its stability upon increased dietary fibre intake in healthy adults. , 2015, Environmental microbiology.

[29]  C. Bernstein,et al.  Antepartum Antibiotic Treatment Increases Offspring Susceptibility to Experimental Colitis: A Role of the Gut Microbiota , 2015, PloS one.

[30]  J. Jiao,et al.  Rumen development process in goats as affected by supplemental feeding v. grazing: age-related anatomic development, functional achievement and microbial colonisation. , 2015, The British journal of nutrition.

[31]  S. Yooseph,et al.  Cultivation of a human-associated TM7 phylotype reveals a reduced genome and epibiotic parasitic lifestyle , 2014, Proceedings of the National Academy of Sciences.

[32]  J. Neary,et al.  Impaired alveolar-arterial oxygen transfer is associated with reduced milk yield in primiparous post-partum dairy heifers at moderate altitude , 2014, Journal of Dairy Research.

[33]  E. Murphy,et al.  Exercise and associated dietary extremes impact on gut microbial diversity , 2014, Gut.

[34]  J. Liu,et al.  Effect of dietary N-carbamoylglutamate on milk production and nitrogen utilization in high-yielding dairy cows. , 2014, Journal of dairy science.

[35]  Itai Sharon,et al.  Metabolic interdependencies between phylogenetically novel fermenters and respiratory organisms in an unconfined aquifer , 2014, The ISME Journal.

[36]  C. Nyachoti,et al.  Dietary supplementation with chitooligosaccharides alters gut microbiota and modifies intestinal luminal metabolites in weaned Huanjiang mini-piglets , 2014 .

[37]  Natalia N. Ivanova,et al.  Insights into the phylogeny and coding potential of microbial dark matter , 2013, Nature.

[38]  Diming Wang,et al.  Potential role of N-carbamoyl glutamate in biosynthesis of arginine and its significance in production of ruminant animals , 2013, Journal of Animal Science and Biotechnology.

[39]  J. Liu,et al.  Insertion depth of oral stomach tubes may affect the fermentation parameters of ruminal fluid collected in dairy cows. , 2012, Journal of dairy science.

[40]  C. McSweeney,et al.  Effects of disodium fumarate on ruminal fermentation and microbial communities in sheep fed on high-forage diets. , 2012, Animal : an international journal of animal bioscience.

[41]  Diming Wang,et al.  Degradation of L-arginine and N-carbamoyl glutamate and their effect on rumen fermentation in vitro , 2012 .

[42]  J. Garssen,et al.  Early life: gut microbiota and immune development in infancy. , 2010, Beneficial microbes.

[43]  B. Wermuth,et al.  Favourable long-term outcome after immediate treatment of neonatal hyperammonemia due to N-acetylglutamate synthase deficiency , 2010, European Journal of Pediatrics.

[44]  S. L. Owens,et al.  Metabolic fates of ammonia-N in ruminal epithelial and duodenal mucosal cells isolated from growing sheep. , 2005, Journal of dairy science.

[45]  D. Rabier,et al.  A new neonatal case ofN-acetylglutamate synthase deficiency treated by carbamylglutamate , 1995, Journal of Inherited Metabolic Disease.

[46]  J. Richalet,et al.  Response of nitric oxide pathway to L-arginine infusion at the altitude of 4,350 m. , 2001, The European respiratory journal.

[47]  C. Wenk,et al.  Recent advances in animal feed additives such as metabolic modifiers, antimicrobial agents, probiotics, enzymes and highly available minerals - review. , 2000 .

[48]  P. V. Soest,et al.  Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. , 1991, Journal of dairy science.

[49]  Kenneth Helrick,et al.  Official methods of analysis , 1990 .

[50]  Sidney A. Williams,et al.  Official Methods of Analysis of the Association of Official Analytical Chemists , 1971, Soil Science Society of America Journal.