Helicase-dependent amplification: use in OnChip amplification and potential for point-of-care diagnostics

Isothermal amplification technologies are emerging on the horizon that could have the potential to pose as alternatives to PCR in terms of sensitivity and ease of use. One of the most recent isothermal technologies is helicase-dependent amplification (HDA). This technology uses the helicase’s capability to disrupt the hydrogen bonds of a Watson–Crick base pair in order to separate dsDNA. A denaturation step, as is used in PCR, is no longer required. This gives rise to new, less expensive and less complicated designs for point-of-care devices and ‘Lab on Chip’ systems. Helicase-dependent OnChip-amplification (OnChip-HDA) is a further step into this direction as it integrates the HDA technology with microarray technology and its power of multiplexing. This special report will give an overview on the HDA and OnChip-HDA technology, and its potential for point-of-care diagnostics.

[1]  H. Kong,et al.  Development of isothermal TaqMan assays for detection of biothreat organisms. , 2008, BioTechniques.

[2]  J. Petrik,et al.  Diagnostic applications of microarrays , 2006, Transfusion medicine.

[3]  M. Huber,et al.  Detection of single base alterations in genomic DNA by solid phase polymerase chain reaction on oligonucleotide microarrays. , 2001, Analytical biochemistry.

[4]  F. Ye,et al.  Amplification of circularizable probes for the detection of target nucleic acids and proteins. , 2006, Clinica chimica acta; international journal of clinical chemistry.

[5]  M. W. Mueller,et al.  Microarray-Based Identification of Bacteria in Clinical Samples by Solid-Phase PCR Amplification of 23S Ribosomal DNA Sequences , 2004, Journal of Clinical Microbiology.

[6]  C. Smith,et al.  Rolling circle amplification of DNA immobilized on solid surfaces and its application to multiplex mutation detection. , 1999, Genetic analysis : biomolecular engineering.

[7]  H. Kong,et al.  Development of a novel one-tube isothermal reverse transcription thermophilic helicase-dependent amplification platform for rapid RNA detection. , 2007, The Journal of molecular diagnostics : JMD.

[8]  L. Bodrossy,et al.  Oligonucleotide microarrays in microbial diagnostics. , 2004, Current opinion in microbiology.

[9]  F. Bier,et al.  On-chip PCR amplification of very long templates using immobilized primers on glassy surfaces. , 2005, Biosensors & bioelectronics.

[10]  F. Bier,et al.  In vitro transcription of a whole gene on a surface-coupled template. , 2005, Lab on a chip.

[11]  Hyun-Jin Kim,et al.  Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification* , 2005, Journal of Biological Chemistry.

[12]  Ying Li,et al.  Enhancing helicase-dependent amplification by fusing the helicase with the DNA polymerase. , 2008, Gene.

[13]  M. W. Mueller,et al.  Accessing single nucleotide polymorphisms in genomic DNA by direct multiplex polymerase chain reaction amplification on oligonucleotide microarrays. , 2002, Analytical biochemistry.

[14]  A. Makarov,et al.  DNA microarrays in the clinic: infectious diseases. , 2008, BioEssays : news and reviews in molecular, cellular and developmental biology.

[15]  Yan Xu,et al.  Helicase‐dependent isothermal DNA amplification , 2004, EMBO reports.

[16]  Bertrand Lemieux,et al.  Identification of Staphylococcus aureus and Determination of Methicillin Resistance Directly from Positive Blood Cultures by Isothermal Amplification and a Disposable Detection Device , 2008, Journal of Clinical Microbiology.

[17]  Da Xing,et al.  Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends , 2007, Nucleic acids research.

[18]  Chunsun Zhang,et al.  PCR microfluidic devices for DNA amplification. , 2006, Biotechnology advances.

[19]  Ling Wang,et al.  Anchored multiplex amplification on a microelectronic chip array , 2000, Nature Biotechnology.

[20]  Frank F Bier,et al.  Helicase dependent OnChip-amplification and its use in multiplex pathogen detection. , 2009, Clinica chimica acta; international journal of clinical chemistry.

[21]  F. Bier,et al.  Reverse transcription-polymerase chain reaction on a microarray: the integrating concept of “active arrays” , 2008, Analytical and bioanalytical chemistry.

[22]  Mahesh Uttamchandani,et al.  Applications of microarrays in pathogen detection and biodefence , 2008, Trends in Biotechnology.

[23]  G. Turcatti,et al.  Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms. , 2000, Nucleic acids research.

[24]  Eva Ehrentreich-Förster,et al.  Marktpotenzial von Point-of-Care-Testing / Market potential of point-of-care testing , 2009 .

[25]  Microarray-based detection of bacteria by on-chip PCR. , 2006, Methods in molecular biology.

[26]  Varshal K. Davé,et al.  Signal amplification by rolling circle amplification on DNA microarrays. , 2001, Nucleic acids research.

[27]  M. Dufva,et al.  Diagnostic and analytical applications of protein microarrays , 2005, Expert review of proteomics.

[28]  Levente Bodrossy,et al.  Highly parallel microbial diagnostics using oligonucleotide microarrays. , 2006, Clinica chimica acta; international journal of clinical chemistry.

[29]  A. Mirzabekov,et al.  Integration of multiple PCR amplifications and DNA mutation analyses by using oligonucleotide microchip. , 2001, Analytical biochemistry.