In situ antibody detection and charge discrimination using aqueous stable pentacene transistor biosensors.

Pentacene-based organic thin-film transistors were used to create highly sensitive, real-time electronic sensors for selective antibody detection. Bovine serum albumin was covalently attached to a modified pentacene surface to selectively detect the label free monoclonal antiBSA. These sensors displayed a high affinity constant (K(A)) of (1.1 ± 3) × 10(7) M(-1) at pH 7, which is 1 order of magnitude higher than those obtained with a highly sensitive surface plasmon resonance spectroscopy detection system. Furthermore, a high degree of discrimination in the hybrid antiBSA charges was achieved at different pH values. This demonstration of fast, label-free, real-time detection of nanoscale biomolecules in aqueous buffer solutions using the organic transistor sensing platform will have a significant impact on high-performance microarrays in addition to discriminating the presence of ionizable groups.

[1]  P. Rouxhet,et al.  Immobilization of microorganisms by adhesion: Interplay of electrostatic and nonelectrostatic interactions , 1987, Biotechnology and bioengineering.

[2]  Yang Yang,et al.  Patterning organic single-crystal transistor arrays , 2006, Nature.

[3]  T. Yoon,et al.  Performance enhancement of organic thin-film transistors by low-energy argon ion beam treatment of gate dielectric surface , 2007 .

[4]  H. Terashima,et al.  Adsorption of bovine serum albumin onto mica surfaces studied by a direct weighing technique , 2003 .

[5]  Bertram Batlogg,et al.  Stable complementary inverters with organic field-effect transistors on Cytop fluoropolymer gate dielectric , 2009 .

[6]  Luisa Torsi,et al.  A sensitivity-enhanced field-effect chiral sensor. , 2008, Nature materials.

[7]  Vivek Subramanian,et al.  Printable polythiophene gas sensor array for low-cost electronic noses , 2006 .

[8]  Huan‐Xiang Zhou,et al.  Fundamental aspects of protein-protein association kinetics. , 2009, Chemical reviews.

[9]  D. J. Harrison,et al.  Monoclonal antibody binding affinity determined by microchip‐based capillary electrophoresis , 1998, Electrophoresis.

[10]  Zhenan Bao,et al.  Water-stable organic transistors and their application in chemical and biological sensors , 2008, Proceedings of the National Academy of Sciences.

[11]  Ute Zschieschang,et al.  High-mobility polymer gate dielectric pentacene thin film transistors , 2002 .

[12]  B. Persson,et al.  Quantitative determination of surface concentration of protein with surface plasmon resonance using radiolabeled proteins , 1991 .

[13]  Mark E. Thompson,et al.  Label-free, electrical detection of the SARS virus N-protein with nanowire biosensors utilizing antibody mimics as capture probes. , 2009, ACS nano.

[14]  Zhenan Bao,et al.  Electronic sensing of vapors with organic transistors , 2001 .

[15]  M. Steward.  The biolgoical significance of antibody affinity. , 1981, Immunology today.

[16]  D. Botstein,et al.  A method for detecting and correcting feature misidentification on expression microarrays , 2004, BMC Genomics.

[17]  I. Langmuir.  THE ADSORPTION OF GASES ON PLANE SURFACES OF GLASS, MICA AND PLATINUM. , 1918 .

[18]  J. Moore,et al.  Application of surface plasmon resonance toward studies of low-molecular-weight antigen-antibody binding interactions. , 2000, Methods.

[19]  S. W. Cho,et al.  Influence of 2-mercapto-5-nitrobenzimidazole treatment on the electronic characteristics of bottom-contact organic field-effect transistors , 2008 .

[20]  H. Sirringhaus,et al.  Air Stable Cross-Linked Cytop Ultrathin Gate Dielectric for High Yield Low-Voltage Top-Gate Organic Field-Effect Transistors , 2010 .

[21]  A. Zydney,et al.  Effect of solution pH on protein transport through ultrafiltration membranes , 1999, Biotechnology and bioengineering.

[22]  J. Jenkins,et al.  Extracting kinetic rate constants from surface plasmon resonance array systems. , 2008, Analytical biochemistry.

[23]  M. Long,et al.  Measuring binding kinetics of surface-bound molecules using the surface plasmon resonance technique. , 2008, Analytical biochemistry.

[24]  A. M. Carmona-Ribeiro,et al.  Interactions between cationic vesicles and serum proteins , 1998 .

[25]  A. Klug,et al.  Designing DNA-binding proteins on the surface of filamentous phage. , 1995, Current opinion in biotechnology.

[26]  J. Micklefield,et al.  Selective covalent protein immobilization: strategies and applications. , 2009, Chemical reviews.

[27]  Pieter Stroeve,et al.  Protein Transport in Nanoporous Membranes Modified with Self-Assembled Monolayers of Functionalized Thiols , 2002 .

[28]  R. Willson,et al.  Association and dissociation kinetics of anti-hen egg lysozyme monoclonal antibodies HyHEL-5 and HyHEL-10. , 1998, Biophysical journal.

[29]  Zhenan Bao,et al.  In Situ, Label‐Free DNA Detection Using Organic Transistor Sensors , 2010, Advanced materials.

[30]  Jang‐Joo Kim,et al.  Air stable C60 based n-type organic field effect transistor using a perfluoropolymer insulator , 2008 .

[31]  W. Knoll,et al.  Interfaces and thin films as seen by bound electromagnetic waves. , 1998, Annual review of physical chemistry.

[32]  S. Kingsmore.  Multiplexed protein measurement: technologies and applications of protein and antibody arrays , 2006, Nature Reviews Drug Discovery.

[33]  Jin Jang,et al.  Solvent effect of the passivation layer on performance of an organic thin-film transistor , 2007 .