Management of root knot nematode (Meloidogyne javanica) by biocontrol agents in two crop rotations

In a microplot field experiment, Pasteuria penetrans and Verticillium chlamydosporium were applied alone or in combinations in two crop rotations for the management of root knot nematode, Meloidogyne javanica. Root knot nematode densities were significantly reduced with combined application followed by solo application of microbial antagonists. Crop yield significantly increased and plant growth was improved by dual treatments of both biocontrol agents. Number of bacterial spores encumbered with root knot juveniles were comparatively higher in P. penetrans applied plots compared to both microbial agents. Rhizosphere and rhizoplane densities of V. chlamydosporium in both crop sequences were significantly increased over the time in plots where fungal antagonists were applied individually compared to other treatments. Variable increase in egg parasitism by V. chlamydosporium was observed in both crop rotations at the final harvest. Key-words: Pasteuria penetrans, Verticillium chlamydosporium, Root knot nematode, Crop rotation.

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