Defective interfering particles with covalently linked [±]RNA induce interferon

Defective interfering (DI) particles of vesicular stomatitis virus which contain covalently linked complementary [+]message and [−]anti-message RNA as a single-stranded ribonucleoprotein complex within the particle, are extremely efficient inducers of interferon. A single particle can induce a quantum yield of interferon. A single molecule of double-stranded RNA presumed to form, at least in part, on entry into the cell is thought to induce interferon synthesis. Conventional [−]RNA DI particles with the same polypeptide composition as [±]RNA DI particles fail to induce interferon.

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