The response of giant phospholipid vesicles to millimeter waves radiation.

Due to the increasing interest in millimeter waves (MMW) applications in medicine and telecommunications, the investigation of their potential biological effects is of utmost importance. Here we report results of the study of interaction between low-intensity radiation at 53.37 GHz and giant vesicles. Direct optical observations of vesicles subjected to irradiation enabled the monitoring in real time of the response of vesicles. Physical changes of vesicles, i.e. elongation, induced diffusion of fluorescent dye di-8-ANEPPS, and increased attractions between vesicles are demonstrated. These effects are reversible and occur only during irradiation with a "switch on" of the effect requiring a short time. Since the average temperature change was very small the effects could not be attributed to thermal mechanisms. We assume that the interaction of MMW with lipid membrane leads to changes at the membrane-water interface, where charged and dipolar residues are located.

[1]  Reinhard Lipowsky,et al.  A practical guide to giant vesicles. Probing the membrane nanoregime via optical microscopy , 2006, Journal of physics. Condensed matter : an Institute of Physics journal.

[2]  K. Schoenbach,et al.  A study on biological effects of low-intensity millimeter waves , 2002 .

[3]  Michael R. Murphy,et al.  Low-intensity millimeter waves as a novel therapeutic modality , 1999 .

[4]  R. Lipowsky,et al.  Morphological transitions of vesicles induced by alternating electric fields. , 2008, Biophysical journal.

[5]  M. Stuchly,et al.  Coaxial Line Reflection Methods for Measuring Dielectric Properties of Biological Substances at Radio and Microwave Frequencies-A Review , 1980, IEEE Transactions on Instrumentation and Measurement.

[6]  Wenxiang Wang,et al.  Do nonthermal biological effects of millimeter waves exist? , 2002, Twenty Seventh International Conference on Infrared and Millimeter Waves.

[7]  R. Adair,et al.  Vibrational resonances in biological systems at microwave frequencies. , 2002, Biophysical journal.

[8]  C. Hines.  Electromagnetic Waves , 2021, Nature.

[9]  D. O. Rudin,et al.  Reconstitution of Cell Membrane Structure in vitro and its Transformation into an Excitable System , 1962, Nature.

[10]  Kao-Cheng Huang,et al.  Millimeter-wave circular polarized beam-steering antenna array for gigabit wireless communications , 2006 .

[11]  R. Massa,et al.  A high-efficiency waveguide applicator for in vitro exposure of mammalian cells at 1.95 GHz , 2006, IEEE Transactions on Microwave Theory and Techniques.

[12]  W. Heyer,et al.  Extremely high frequency electromagnetic fields at low power density do not affect the division of exponential phase Saccharomyces cerevisiae cells. , 1997, Bioelectromagnetics.

[13]  R. Clarke,et al.  Optical detection of membrane dipole potential: avoidance of fluidity and dye-induced effects. , 1997, Biochimica et biophysica acta.

[14]  N. D. Devyatkov,et al.  Low intensity millimeter waves in medicine and biology. , 2000, Critical reviews in biomedical engineering.

[15]  L. Taylor.  The mechanisms of athermal microwave biological effects. , 1981, Bioelectromagnetics.

[16]  Igor Belyaev,et al.  Non-thermal Biological Effects of Microwaves , 2005 .

[17]  A.W. Guy,et al.  Safety Level with Respect to Human Exposure to Radiofrequency Electromagnetic Fields (300 Khz-100 GHz) , 1980, 1980 IEEE MTT-S International Microwave symposium Digest.

[18]  M C Ziskin,et al.  Millimeter microwave effect on ion transport across lipid bilayer membranes. , 1995, Bioelectromagnetics.

[19]  E. Skafidas,et al.  Characterization of the 60 GHz Wireless Desktop Channel , 2007, IEEE Transactions on Antennas and Propagation.

[20]  M. Ziskin,et al.  Temperature oscillations in liquid media caused by continuous (nonmodulated) millimeter wavelength electromagnetic irradiation. , 1996, Bioelectromagnetics.

[21]  S. J. WEBB,et al.  Inhibition of Bacterial Cell Growth by 136 gc Microwaves , 1968, Nature.

[22]  T. Weiland,et al.  A practical guide to 3-D simulation , 2008, IEEE Microwave Magazine.

[23]  E. E. Fesenko,et al.  Dual effects of microwaves on single Ca2+‐activated K+ channels in cultured kidney cells Vero , 1995, FEBS Letters.

[24]  L. Le Coq,et al.  Interactions between 60-GHz millimeter waves and artificial biological membranes: dependence on radiation parameters , 2006, IEEE Transactions on Microwave Theory and Techniques.

[25]  Pasquale Stano,et al.  Permeability changes induced by 130 GHz pulsed radiation on cationic liposomes loaded with carbonic anhydrase , 2007, Bioelectromagnetics.

[26]  F. Pincet,et al.  Hemifusion and fusion of giant vesicles induced by reduction of inter-membrane distance , 2004, The European physical journal. E, Soft matter.

[27]  Pier Luigi Luisi,et al.  Giant Vesicles as Biochemical Compartments: The Use of Microinjection Techniques , 1998 .

[28]  S. Hadjiloucas,et al.  Preliminary results on the non-thermal effects of 200-350 GHz radiation on the growth rate of S. cerevisiae cells in microcolonies. , 2002, Physics in medicine and biology.

[29]  M C Ziskin,et al.  Effects of millimeter waves on ionic currents of Lymnaea neurons. , 1999, Bioelectromagnetics.

[30]  G. Le Goff,et al.  Orientational polarisability of lipid membrane surfaces. , 2007, Biochimica et biophysica acta.

[31]  W. Grundler,et al.  Resonant growth rate response of yeast cells irradiated by weak microwaves , 1977 .

[32]  Daniel J. Estes,et al.  Electroformation of giant liposomes from spin-coated films of lipids. , 2005, Colloids and surfaces. B, Biointerfaces.

[33]  C. Lüpfert,et al.  Influence of anions and cations on the dipole potential of phosphatidylcholine vesicles: a basis for the Hofmeister effect. , 1999, Biophysical journal.

[34]  Stanislav I. Alekseev,et al.  Distortion of millimeter-wave absorption in biological media due to presence of thermocouples and other objects , 2001, IEEE Transactions on Biomedical Engineering.

[35]  Charles Polk,et al.  CRC Handbook of Biological Effects of Electromagnetic Fields , 1986 .

[36]  R. Adair,et al.  Biophysical limits on athermal effects of RF and microwave radiation , 2003, Bioelectromagnetics.

[37]  Lin Yang,et al.  Observation of a Membrane Fusion Intermediate Structure , 2002, Science.

[38]  M C Ziskin,et al.  Medical application of millimetre waves. , 1998, QJM : monthly journal of the Association of Physicians.

[39]  Y. Akyel,et al.  Current state and implications of research on biological effects of millimeter waves: a review of the literature. , 1998, Bioelectromagnetics.

[40]  S. Svetina,et al.  The response of giant phospholipid vesicles to pore-forming peptide melittin. , 2007, Biochimica et biophysica acta.

[41]  Mirianas Chachisvilis,et al.  Laurdan fluorescence senses mechanical strain in the lipid bilayer membrane. , 2006, Biochemical and biophysical research communications.

[42]  H. Fröhlich,et al.  Biological coherence and response to external stimuli , 1988 .

[43]  J. Zhao,et al.  Numerical dosimetry for cells under millimetre-wave irradiation using Petri dish exposure set-ups , 2005, Physics in medicine and biology.