Intramural Measurement of Transmembrane Potential in the Isolated Pig Heart: Validation of a Novel Technique

Introduction: Transmembrane potentials can be recorded at multiple intramural sites in the intact heart using fiber optic probes or optrodes. The technique has considerable potential utility for studies of arrhythmia and defibrillation, but has not been validated in large mammalian hearts.

[1]  D. Durrer,et al.  Spread of activation in the left ventricular wall of the dog. I. , 1953, American heart journal.

[2]  W. L. Kirk,et al.  Propagation Velocities and Voltage Magnitudes in Local Segments of Dog Myocardium , 1970, Circulation research.

[3]  R. Holland,et al.  The QRS complex during myocardial ischemia. An experimental analysis in the porcine heart. , 1976, The Journal of clinical investigation.

[4]  G. Salama,et al.  Optical probes of membrane potential in heart muscle. , 1979, The Journal of physiology.

[5]  M. Allessie,et al.  Electrophysiologic mapping to determine the mechanism of experimental ventricular tachycardia initiated by premature impulses. Experimental approach and initial results demonstrating reentrant excitation. , 1982, The American journal of cardiology.

[6]  M. Spach,et al.  Relating Extracellular Potentials and Their Derivatives to Anisotropic Propagation at a Microscopic Level in Human Cardiac Muscle: Evidence for Electrical Uncoupling of Side‐to‐Side Fiber Connections with Increasing Age , 1986, Circulation research.

[7]  P. Wolf,et al.  Transmural activations and stimulus potentials in three-dimensional anisotropic canine myocardium. , 1988, Circulation research.

[8]  R. Lux,et al.  Correlation between in vivo transmembrane action potential durations and activation-recovery intervals from electrograms. Effects of interventions that alter repolarization time. , 1990, Circulation.

[9]  A. Wilde,et al.  Morphology of electrophysiologically identified junctions between Purkinje fibers and ventricular muscle in rabbit and pig hearts. , 1991, Circulation research.

[10]  R. Lux,et al.  Epicardial Potential Mapping: Effects of Conducting Media on Isopotential and Isochrone Distributions , 1991, Circulation.

[11]  T. Opthof,et al.  Injury current and gradients of diastolic stimulation threshold, TQ potential, and extracellular potassium concentration during acute regional ischemia in the isolated perfused pig heart. , 1991, Circulation research.

[12]  S. Dillon.  Synchronized Repolarization After Defibrillation Shocks: A Possible Component of the Defibrillation Process Demonstrated by Optical Recordings in Rabbit Heart , 1992, Circulation.

[13]  J. Davidenko,et al.  Effects of diacetyl monoxime on the electrical properties of sheep and guinea pig ventricular muscle. , 1993, Cardiovascular research.

[14]  R. Gray,et al.  Incomplete reentry and epicardial breakthrough patterns during atrial fibrillation in the sheep heart. , 1996, Circulation.

[15]  C. Antzelevitch,et al.  Evidence for the Presence of M Cells in the Guinea Pig Ventricle , 1996, Journal of cardiovascular electrophysiology.

[16]  D. Rosenbaum,et al.  Unique Properties of Cardiac Action Potentials Recorded with Voltage‐Sensitive Dyes , 1996, Journal of cardiovascular electrophysiology.

[17]  G. Salama,et al.  Activation and Repolarization Patterns are Governed by Different Structural Characteristics of Ventricular Myocardium: , 1996, Journal of cardiovascular electrophysiology.

[18]  A. Rodríguez-Sinovas,et al.  Lack of evidence of M-cells in porcine left ventricular myocardium. , 1997, Cardiovascular research.

[19]  M. Fishbein,et al.  Cellular graded responses and ventricular vulnerability to reentry by a premature stimulus in isolated canine ventricle. , 1997, Circulation.

[20]  C. Antzelevitch,et al.  Characteristics and distribution of M cells in arterially perfused canine left ventricular wedge preparations. , 1998, Circulation.

[21]  R. K. Justice,et al.  Ratiometry of transmembrane voltage-sensitive fluorescent dye emission in hearts. , 2000, American journal of physiology. Heart and circulatory physiology.

[22]  R. Ideker,et al.  Mechanism of Ventricular Defibrillation for Near-Defibrillation Threshold Shocks: A Whole-Heart Optical Mapping Study in Swine , 2001, Circulation.

[23]  C. Antzelevitch.  Transmural dispersion of repolarization and the T wave. , 2001, Cardiovascular research.

[24]  I. LeGrice,et al.  Intramural multisite recording of transmembrane potential in the heart. , 2001, Biophysical journal.

[25]  S. F. Mironov,et al.  Visualizing excitation waves inside cardiac muscle using transillumination. , 2001, Biophysical journal.

[26]  Jian Huang,et al.  Fiberglass needle electrodes for transmural cardiac mapping , 2002, IEEE Transactions on Biomedical Engineering.

[27]  D. Rosenbaum,et al.  Transmural Electrophysiological Heterogeneities Underlying Arrhythmogenesis in Heart Failure , 2003, Circulation research.

[28]  R. Ideker,et al.  Development of an Optrode for Intramural Multisite Optical Recordings of Vm in the Heart , 2003, Journal of cardiovascular electrophysiology.

[29]  R. Ideker,et al.  Effects of heart isolation, voltage-sensitive dye, and electromechanical uncoupling agents on ventricular fibrillation. , 2003, American journal of physiology. Heart and circulatory physiology.

[30]  L. Tung,et al.  Design and use of an “optrode” for optical recordings of cardiac action potentials , 1992, Pflügers Archiv.

[31]  G. Salama,et al.  A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations , 1992, The Journal of Membrane Biology.

[32]  I. LeGrice,et al.  Correction of motion artifact in transmembrane voltage-sensitive fluorescent dye emission in hearts. , 2004, American journal of physiology. Heart and circulatory physiology.