Repeated pulse feeding induces functional stability in anaerobic digestion

Anaerobic digestion is an environmental key technology in the future bio‐based economy. To achieve functional stability, a minimal microbial community diversity is required. This microbial community should also have a certain ‘elasticity’, i.e. the ability to rapidly adapt to suboptimal conditions or stress. In this study it was evaluated whether a higher degree of functional stability could be achieved by changing the feeding pattern, which can change the evenness, dynamics and richness of the bacterial community. The first reactor (CSTRstable) was fed on daily basis, whereas the second reactor (CSTRdynamic) was fed every 2 days. Average biogas production was 0.30 l CH4 l−1 day−1 in both reactors, although daily variation was up to four times higher in the CSTRdynamic compared with the CSTRstable during the first 50 days. Bacterial analysis revealed that this CSTRdynamic had a two times higher degree of bacterial community dynamics. The CSTRdynamic also appeared to be more tolerant to an organic shock load of 8 g COD l−1 and ammonium levels up to 8000 mg TAN l−1. These results suggest that the regular application of a limited pulse of organic material and/or a variation in the substrate composition might promote higher functional stability in anaerobic digestion.

[1]  W. Verstraete,et al.  Evaluation of nested PCR-DGGE (denaturing gradient gel electrophoresis) with group-specific 16S rRNA primers for the analysis of bacterial communities from different wastewater treatment plants. , 2002, FEMS microbiology ecology.

[2]  S. Schmidt,et al.  Potential impact of process parameters upon the bacterial diversity in the mesophilic anaerobic digestion of beet silage. , 2011, Bioresource technology.

[3]  P. de Vos,et al.  Bioaugmentation of Activated Sludge by an Indigenous 3-Chloroaniline-Degrading Comamonas testosteroni Strain, I2gfp , 2000, Applied and Environmental Microbiology.

[4]  W. Verstraete,et al.  Initial community evenness favours functionality under selective stress , 2009, Nature.

[5]  M. Marzorati,et al.  Microbial Resource Management revisited: successful parameters and new concepts , 2011, Applied Microbiology and Biotechnology.

[6]  Willy Verstraete,et al.  Methanosarcina: the rediscovered methanogen for heavy duty biomethanation. , 2012, Bioresource technology.

[7]  W. Verstraete,et al.  A time-course analysis of four full-scale anaerobic digesters in relation to the dynamics of change of their microbial communities. , 2011, Water science and technology : a journal of the International Association on Water Pollution Research.

[8]  W Verstraete,et al.  Anaerobic digestion as a core technology in sustainable management of organic matter. , 2005, Water science and technology : a journal of the International Association on Water Pollution Research.

[9]  Jaai Kim,et al.  Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction. , 2005, Biotechnology and bioengineering.

[10]  Willy Verstraete,et al.  Sedimentological evolution in an UASB treating SYNTHES, a new representative synthetic sewage, at low loading rates. , 2004, Bioresource technology.

[11]  M. Gerardi.  Anaerobic Digestion Stages , 2003 .

[12]  L. Raskin,et al.  Diversity and dynamics of microbial communities in engineered environments and their implications for process stability. , 2003, Current opinion in biotechnology.

[13]  J. Holm‐Nielsen,et al.  The future of anaerobic digestion and biogas utilization. , 2009, Bioresource technology.

[14]  W. Verstraete,et al.  Quantifying Community Dynamics of Nitrifiers in Functionally Stable Reactors , 2007, Applied and Environmental Microbiology.

[15]  M. Marzorati,et al.  Nutrient gradients in a granular activated carbon biofilter drives bacterial community organization and dynamics. , 2011, Water research.

[16]  S. Haruta,et al.  Microbial community changes during organic solid waste treatment analyzed by double gradient-denaturing gradient gel electrophoresis and fluorescence in situ hybridization , 2002, Applied Microbiology and Biotechnology.

[17]  Joan Mata-Álvarez,et al.  Anaerobic digestion of organic solid wastes. An overview of research achievements and perspectives , 2000 .

[18]  A. Uitterlinden,et al.  Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA , 1993, Applied and environmental microbiology.

[19]  R. Moletta,et al.  Monitoring of activity dynamics of an anaerobic digester bacterial community using 16S rRNA polymerase chain reaction--single-strand conformation polymorphism analysis. , 2000, Environmental microbiology.

[20]  C. Visvanathan,et al.  Effect of organic loading rate on VFA production, organic matter removal and microbial activity of a two-stage thermophilic anaerobic membrane bioreactor. , 2011, Bioresource technology.

[21]  W. Verstraete,et al.  Correlations between molecular and operational parameters in continuous lab-scale anaerobic reactors , 2010, Applied Microbiology and Biotechnology.

[22]  Awwa,et al.  Standard Methods for the examination of water and wastewater , 1999 .

[23]  P. Marschner,et al.  Methane production and microbial community structure in single-stage batch and sequential batch systems anaerobically co-digesting food waste and biosolids , 2005, Applied Microbiology and Biotechnology.

[24]  H. D. Stensel,et al.  Growth Kinetics and Competition Between Methanosarcina and Methanosaeta in Mesophilic Anaerobic Digestion , 2006, Water environment research : a research publication of the Water Environment Federation.

[25]  E. Pelletier,et al.  Towards the definition of a core of microorganisms involved in anaerobic digestion of sludge , 2009, The ISME Journal.

[26]  Suiying Huang,et al.  How Stable Is Stable? Function versus Community Composition , 1999, Applied and Environmental Microbiology.

[27]  J P Steyer,et al.  First step towards a fast analytical method for the determination of Biochemical Methane Potential of solid wastes by near infrared spectroscopy. , 2011, Bioresource technology.

[28]  Willy Verstraete,et al.  How to get more out of molecular fingerprints: practical tools for microbial ecology. , 2008, Environmental microbiology.

[29]  Willy Verstraete,et al.  Microbial Resource Management: The Road To Go for Environmental Biotechnology , 2007 .

[30]  C. S. Holling,et al.  Ecological Resilience, Biodiversity, and Scale , 1998, Ecosystems.

[31]  Willy Verstraete,et al.  Anaerobic digestibility of marine microalgae Phaeodactylum tricornutum in a lab-scale anaerobic membrane bioreactor , 2011, Applied Microbiology and Biotechnology.