Molecular characterization of 125I decay and X-ray-induced HPRT mutants in CHO cells.

The predominant initial lesion induced by 125I decay in DNa is a double-strand DNA break comprising a 'mini deletion' of up to several base pairs (bp). However, Southern analysis of the structure of 125I decay-induced HPRT mutants in Chinese hamster ovary cells revealed that most of the mutants are deletions of thousands of bps. A similar predominance of substantial deletions was also found in X-ray-induced mutants.

[1]  D. Charlton.  The range of high LET effects from 125I decays. , 1986, Radiation research.

[2]  A. Morley,et al.  Molecular basis of X-ray-induced mutation at the HPRT locus in human lymphocytes. , 1986, Mutation research.

[3]  J. Thacker.  The nature of mutants induced by ionising radiation in cultured hamster cells. III. Molecular characterization of HPRT-deficient mutants induced by gamma-rays or alpha-particles showing that the majority have deletions of all or part of the hprt gene. , 1986, Mutation research.

[4]  K. Tindall,et al.  Quantitative and molecular analyses of ethyl methanesulfonate- and ICR 191-induced mutation in AS52 cells. , 1986, Mutation research.

[5]  C. H. Ockey,et al.  Molecular analysis of X-ray-induced mutants at the HPRT locus in V79 Chinese hamster cells. , 1986, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[6]  L. Stankowski,et al.  Quantitative and molecular analyses of radiation-induced mutation in AS52 cells. , 1986, Radiation research.

[7]  H. Vrieling,et al.  Mutations induced by X-rays at the HPRT locus in cultured Chinese hamster cells are mostly large deletions. , 1985, Mutation research.

[8]  R. Albertini,et al.  Alterations of the hprt gene in human in vivo-derived 6-thioguanine-resistant T lymphocytes , 1985, Nature.

[9]  A. Morley,et al.  In vivo somatic mutations in human lymphocytes frequently result from major gene alterations , 1985, Nature.

[10]  I. Radford.  The level of induced DNA double-strand breakage correlates with cell killing after X-irradiation. , 1985, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[11]  J. Thacker,et al.  The nature of mutants by ionising radiation in cultured hamster cells , 1984 .

[12]  P. Brookes,et al.  On the nature of the mutations induced by the diolepoxide of benzo[a]pyrene in mammalian cells. , 1984, Carcinogenesis.

[13]  C. Caskey,et al.  Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[14]  H. Liber,et al.  Toxicity and mutagenicity of X-rays and [125I]dUrd or [3H]TdR incorporated in the DNA of human lymphoblast cells. , 1983, Mutation research.

[15]  C. Caskey,et al.  Expression of human and Chinese hamster hypoxanthine-guanine phosphoribosyltransferase cDNA recombinants in cultured Lesch-Nyhan and Chinese hamster fibroblasts. , 1983, The Journal of biological chemistry.

[16]  E. Käs,et al.  Deletion of the diploid dihydrofolate reductase locus from cultured mammalian cells , 1983, Cell.

[17]  D. Ledbetter,et al.  Deletion and amplification of the HGPRT locus in Chinese hamster cells , 1983, Molecular and cellular biology.

[18]  Jack W. Szostak,et al.  The double-strand-break repair model for recombination , 1983, Cell.

[19]  R. Martin,et al.  Use of an 125I-labelled DNA ligand to probe DNA structure , 1983, Nature.

[20]  A. C. Chinault,et al.  Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants. , 1982, Nucleic acids research.

[21]  A. C. Chinault,et al.  Cloned cDNA sequences of the hypoxanthine/guanine phosphoribosyltransferase gene from a mouse neuroblastoma cell line found to have amplified genomic sequences. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[22]  Y. Fujiwara,et al.  Mutagenic and lethal effects of [5-125I]iodo-2'-deoxyuridine incorporated into DNA of mammalian cells, and their RBEs. , 1981, Radiation research.

[23]  W. Haseltine,et al.  Range of radiochemical damage to DNA with decay of iodine-125. , 1981, Science.

[24]  G. Buttin,et al.  The potentiation of adenine toxicity to Chinese hamster cells by coformycin: Suppression in mutants with altered regulation of purine biosynthesis or increased adenylate‐deaminase activity , 1981, Journal of cellular physiology.

[25]  S. Cory,et al.  Deletions are associated with somatic rearrangement of immunoglobulin heavy chain genes , 1980, Cell.

[26]  K. Hofer,et al.  Biological toxicity of Auger emitters: molecular fragmentation versus electron irradiation. , 1978, Current topics in radiation research quarterly.

[27]  N. Blin,et al.  A general method for isolation of high molecular weight DNA from eukaryotes. , 1976, Nucleic acids research.

[28]  R. Krisch,et al.  Further studies of DNA damage and lethality from the decay of iodine-125 in bacteriophages. , 1975, International journal of radiation biology and related studies in physics, chemistry, and medicine.