论文信息 - Automated quantification of choriogonadotropin: analytical correlation between serum and urine with creatinine correction.

Automated quantification of choriogonadotropin: analytical correlation between serum and urine with creatinine correction.

We evaluated the Boehringer Mannheim Diagnostics ES 600 automated immunoassay system for measuring choriogonadotropin (hCG) in human serum, plasma, and urine. The ES 600, an automated sample-selective, multibatch analyzer, is based on immunoenzymometric coated-tube technology. Interassay CVs for assay of both serum and urine ranged from 2% to 16%. The analytical sensitivity was 0.5 int. unit/L. Results for serum correlated well with qualitative "Icon" and quantitative "Tandem" assays (Hybritech). In addition, results for serum and urinary hCG correlated well when the latter was expressed in terms of creatinine excretion. These results suggest that determination of hCG in urine can be correlated with those in serum for use as a marker for chorionic tumors or for screening abnormal pregnancies, where the rate of hCG production may be important.

D. Chan | A. Wu | S. Wong | C. Waldron

[1] J. Nielsen,et al. Evaluation and comparison of commercially available pregnancy tests based on monoclonal antibodies to human choriogonadotropin. , 1986, Clinical chemistry.

[2] R. Romero,et al. Progesterone, estradiol, and alpha-human chorionic gonadotropin secretion in patients with ectopic pregnancy. , 1986, The Journal of clinical endocrinology and metabolism.

[3] R. Hussa,et al. "Tandem ICON hCG" urine pregnancy test evaluated. , 1985, Clinical chemistry.

[4] R. Hussa,et al. Discordant Human Chorionic Gonadotropin Results: Causes and Solutions , 1985, Obstetrics and gynecology.

[5] V. Caride,et al. Discordant inter-kit results in the radioimmunoassay for choriogonadotropin in serum. , 1984, Clinical chemistry.

[6] R. Romero,et al. A Method of Screening for Ectopic Pregnancy and Its Indications , 1981, Obstetrics and gynecology.

[7] M. Roger,et al. Measurement of plasma human chorionic gonadotropin (hCG) and beta-hCG activities in the late luteal phase: evidence of the occurrence of spontaneous menstrual abortions in infertile women. , 1979, Fertility and sterility.