Regulation of specific functions of glial cells in somatic hybrids. I. Control of S100 protein

Hybrids were isolated between rat glial cells and mouse fibroblasts. Micro complement (C′) fixation was used to assay S100, a highly acidic protein specific for nervous tissues. The glial cells contain large amounts of S100. Extracts of the fibroblasts contain some C′ fixing material which is detected only at very high protein concentrations and which fixes C′ only weakly. The identity of this material is not known. The hybrids contain some C′ fixing material, but the concentrations of protein necessary to reach the point of antigen‐antibody equivalence is ten times greater with an extract of hybrids than with an extract of glial cells. This increase in the concentration of protein is associated with a decrease in the amount of C′ fixed. The possible significance of the C′ fixing material in the hybrids is discussed.

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