Single-monomer formulation of polymerized polyethylene glycol diacrylate as a nonadsorptive material for microfluidics.

Nonspecific adsorption in microfluidic systems can deplete target molecules in solution and prevent analytes, especially those at low concentrations, from reaching the detector. Polydimethylsiloxane (PDMS) is a widely used material for microfluidics, but it is prone to nonspecific adsorption, necessitating complex chemical modification processes to address this issue. An alternative material to PDMS that does not require subsequent chemical modification is presented here. Poly(ethylene glycol) diacrylate (PEGDA) mixed with photoinitiator forms on exposure to ultraviolet (UV) radiation a polymer with inherent resistance to nonspecific adsorption. Optimization of the polymerized PEGDA (poly-PEGDA) formula imbues this material with some of the same properties, including optical clarity, water stability, and low background fluorescence, that make PDMS so popular. Poly-PEGDA demonstrates less nonspecific adsorption than PDMS over a range of concentrations of flowing fluorescently tagged bovine serum albumin solutions, and poly-PEGDA has greater resistance to permeation by small hydrophobic molecules than PDMS. Poly-PEGDA also exhibits long-term (hour scale) resistance to nonspecific adsorption compared to PDMS when exposed to a low (1 μg/mL) concentration of a model adsorptive protein. Electrophoretic separations of amino acids and proteins resulted in symmetrical peaks and theoretical plate counts as high as 4 × 10(5)/m. Poly-PEGDA, which displays resistance to nonspecific adsorption, could have broad use in small volume analysis and biomedical research.

[1]  P. Silberzan,et al.  Microfluidics for biotechnology , 2005 .

[2]  S. Cannistraro,et al.  A dynamic light scattering study on mutual diffusion coefficient of BSA in concentrated aqueous solutions , 1998 .

[3]  Helene Andersson-Svahn,et al.  Overview of single-cell analyses: microdevices and applications. , 2010, Lab on a chip.

[4]  Jing-Juan Xu,et al.  In-situ grafting hydrophilic polymer on chitosan modified poly(dimethylsiloxane) microchip for separation of biomolecules. , 2007, Journal of chromatography. A.

[5]  G. Whitesides,et al.  Low-cost printing of poly(dimethylsiloxane) barriers to define microchannels in paper. , 2008, Analytical chemistry.

[6]  Daniel T Chiu,et al.  Rapid prototyping of thermoset polyester microfluidic devices. , 2004, Analytical chemistry.

[7]  John A Rogers,et al.  Soft lithography using acryloxy perfluoropolyether composite stamps. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[8]  Chang-Soo Lee,et al.  Solvent‐Resistant PDMS Microfluidic Devices with Hybrid Inorganic/Organic Polymer Coatings , 2009 .

[9]  R. Kennedy,et al.  Parallel electrophoretic analysis of segmented samples on chip for high-throughput determination of enzyme activities. , 2010, Analytical chemistry.

[10]  Darwin R. Reyes,et al.  Micro total analysis systems. 1. Introduction, theory, and technology. , 2002, Analytical chemistry.

[11]  A. Majumdar,et al.  Stamp-and-stick room-temperature bonding technique for microdevices , 2005, Journal of Microelectromechanical Systems.

[12]  Armand Ajdari,et al.  Stable modification of PDMS surface properties by plasma polymerization: application to the formation of double emulsions in microfluidic systems. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[13]  Lloyd M Smith,et al.  Integrated microfluidic device for automated single cell analysis using electrophoretic separation and electrospray ionization mass spectrometry. , 2010, Analytical chemistry.

[14]  Holger Schönherr,et al.  Nanoscale hydrophobic recovery: A chemical force microscopy study of UV/ozone-treated cross-linked poly(dimethylsiloxane). , 2004, Langmuir : the ACS journal of surfaces and colloids.

[15]  R. Oleschuk,et al.  Surface modification of poly(dimethylsiloxane) with a perfluorinated alkoxysilane for selectivity toward fluorous tagged peptides. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[16]  P. Willis,et al.  Monolithic photolithographically patterned Fluorocur PFPE membrane valves and pumps for in situ planetary exploration. , 2008, Lab on a chip.

[17]  S. Howorka,et al.  Glass surfaces grafted with high-density poly(ethylene glycol) as substrates for DNA oligonucleotide microarrays. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[18]  Ali Khademhosseini,et al.  Fabrication of non-biofouling polyethylene glycol micro- and nanochannels by ultraviolet-assisted irreversible sealing. , 2006, Lab on a chip.

[19]  G. Whitesides,et al.  Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). , 1998, Analytical chemistry.

[20]  A. Woolley,et al.  In‐channel atom‐transfer radical polymerization of thermoset polyester microfluidic devices for bioanalytical applications , 2007, Electrophoresis.

[21]  Wenming Liu,et al.  Microfluidics: a new cosset for neurobiology. , 2009, Lab on a chip.

[22]  F. Lagugné-Labarthet,et al.  Surface modification of poly(dimethylsiloxane) for microfluidic assay applications , 2010 .

[23]  Adam T Woolley,et al.  Microdevices integrating affinity columns and capillary electrophoresis for multibiomarker analysis in human serum. , 2010, Lab on a chip.

[24]  Bingcheng Lin,et al.  Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans. , 2010, Lab on a chip.

[25]  Dhananjay Bodas,et al.  Formation of more stable hydrophilic surfaces of PDMS by plasma and chemical treatments , 2006 .

[26]  M. Gijs,et al.  Three-dimensional magnetic focusing of superparamagnetic beads for on-chip agglutination assays. , 2011, Analytical chemistry.

[27]  Po Ki Yuen,et al.  Perfusion-based microfluidic device for three-dimensional dynamic primary human hepatocyte cell culture in the absence of biological or synthetic matrices or coagulants. , 2010, Lab on a chip.

[28]  Hongkai Wu,et al.  Coating of poly(dimethylsiloxane) with n-dodecyl-beta-D-maltoside to minimize nonspecific protein adsorption. , 2005, Lab on a chip.

[29]  C. Culbertson,et al.  Sol-gel modified poly(dimethylsiloxane) microfluidic devices with high electroosmotic mobilities and hydrophilic channel wall characteristics. , 2005, Analytical chemistry.

[30]  Xuefei Sun,et al.  Adsorption-resistant acrylic copolymer for prototyping of microfluidic devices for proteins and peptides. , 2007, Analytical chemistry.

[31]  Jinyi Wang,et al.  Solution-phase surface modification in intact poly(dimethylsiloxane) microfluidic channels. , 2006, Analytical Chemistry.

[32]  Adam T Woolley,et al.  Thermal bonding of polymeric capillary electrophoresis microdevices in water. , 2003, Analytical chemistry.

[33]  M. Owen,et al.  Behavior of Plasma-Treated Elastomeric Polydimethylsiloxane Coatings in Aqueous Environment , 1998 .

[34]  M. Fenech,et al.  Sorted cell microarrays as platforms for high-content informational bioassays. , 2010, Lab on a chip.

[35]  T. Lindahl,et al.  Fluorescence-based blood coagulation assay device for measuring activated partial thromboplastin time. , 2011, Analytical chemistry.

[36]  N. Allbritton,et al.  Air-stable supported membranes for single-cell cytometry on PDMS microchips. , 2010, Lab on a chip.

[37]  C. Culbertson,et al.  Surface engineering of poly(dimethylsiloxane) microfluidic devices using transition metal sol-gel chemistry. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[38]  Richard A Mathies,et al.  Multichannel capillary electrophoresis microdevice and instrumentation for in situ planetary analysis of organic molecules and biomarkers. , 2010, Analytical chemistry.

[39]  Daniel T Chiu,et al.  Ultrasensitive and high-throughput fluorescence analysis of droplet contents with orthogonal line confocal excitation. , 2010, Analytical chemistry.

[40]  G. Whitesides,et al.  Microfluidic devices fabricated in Poly(dimethylsiloxane) for biological studies , 2003, Electrophoresis.

[41]  A. Plant,et al.  Reproducibility and robustness of a real-time microfluidic cell toxicity assay. , 2011, Analytical chemistry.

[42]  Mark Bachman,et al.  Photoresist with low fluorescence for bioanalytical applications. , 2007, Analytical chemistry.

[43]  Dan Bratton,et al.  Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays. , 2009, Lab on a chip.

[44]  Andreas Manz,et al.  Latest developments in micro total analysis systems. , 2010, Analytical chemistry.