Mapping of immunoreactive sites of pneumococcal pneumolysin by use of synthetic peptides

In the search for better diagnostic tools and improved vaccines against pneumococcal diseases, continuous antigenic sites of the pneumococcal pneumolysin molecule were identified with partially overlapping synthetic peptides. Peptides were derived from the predicted amino acid sequence according to the known DNA sequence of pneumolysin. The immunoreactivities of the peptides were studied by enzyme immunoassay with seven human serum samples and three rabbit hyperimmune serum samples. Two immunoreactive sites in the overlapping peptides at the amino-terminal end of the pneumolysin molecule were identified in this study. Two common immunoreactive sites for both human and rabbit sera were demonstrated.

[1]  G. Boulnois,et al.  Structure and function of pneumolysin, the multifunctional, thiol‐activated toxin of Streptococcus pneumoniae , 1991, Molecular microbiology.

[2]  P. Andrew,et al.  Complement activation and antibody binding by pneumolysin via a region of the toxin homologous to a human acute‐phase protein , 1991, Molecular microbiology.

[3]  M. Leinonen,et al.  Pneumolysin, produced in Bacillus subtilis as antigen in enzyme immunoassay for etiological diagnosis of pneumococcal pneumonia , 1990 .

[4]  M. Sarvas,et al.  Production of pneumolysin, a pneumococcal toxin, in Bacillus subtilis. , 1989, Gene.

[5]  A. Vaheri,et al.  Synthetic env gp41 peptide as a sensitive and specific diagnostic reagent in different stages of human immunodeficiency virus type 1 infection , 1988, Journal of medical virology.

[6]  M. Ward,et al.  Epitope mapping with solid‐phase peptides: identification of type‐, subspecies‐, species‐ and genus‐reactive antibody binding domains on the major outer membrane protein of Chlamydia trachomatis , 1988, Molecular microbiology.

[7]  M. Granström,et al.  Serum antibodies to pneumolysin in patients with pneumonia , 1988, Journal of clinical microbiology.

[8]  M. Granström,et al.  Immunological relation between serum antibodies against pneumolysin and against streptolysin O. , 2009, Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology.

[9]  G. Boulnois,et al.  Nucleotide sequence of the streptolysin O (SLO) gene: structural homologies between SLO and other membrane-damaging, thiol-activated toxins , 1987, Infection and immunity.

[10]  H. M. Geysen,et al.  Strategies for epitope analysis using peptide synthesis. , 1987, Journal of immunological methods.

[11]  G. Boulnois,et al.  Molecular cloning, characterization, and complete nucleotide sequence of the gene for pneumolysin, the sulfhydryl-activated toxin of Streptococcus pneumoniae , 1987, Infection and immunity.

[12]  M. Granström,et al.  Diagnosis of pneumococcal pneumonia by enzyme-linked immunosorbent assay of antibodies to pneumococcal hemolysin (pneumolysin) , 1987, Journal of clinical microbiology.

[13]  M. Leinonen,et al.  The aetiology of pneumonia. Application of bacterial serology and basic laboratory methods. , 1987, The Journal of infection.

[14]  P. Manning,et al.  Cloning and expression in Escherichia coli of the Streptococcus pneumoniae gene encoding pneumolysin , 1986, Infection and immunity.

[15]  A. Ferrante,et al.  Inhibition of in vitro human lymphocyte response by the pneumococcal toxin pneumolysin , 1984, Infection and immunity.

[16]  Curtis N. James,et al.  Activation of human complement by the pneumococcal toxin pneumolysin , 1984, Infection and immunity.

[17]  R. Austrian,et al.  9 – Pneumococcal Infections , 1984 .

[18]  A. Ferrante,et al.  Inhibition of human polymorphonuclear leukocyte respiratory burst, bactericidal activity, and migration by pneumolysin , 1983, Infection and immunity.

[19]  J. Paton,et al.  Effect of immunization with pneumolysin on survival time of mice challenged with Streptococcus pneumoniae , 1983, Infection and immunity.

[20]  M. Sarvas,et al.  Secretion of Escherichia coli beta-lactamase from Bacillus subtilis by the aid of alpha-amylase signal sequence. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[21]  J. T. Smeall.  Pneumococcal Infections , 1949, Edinburgh medical journal.