Characterisation and properties of an intracellular lipid-binding protein from the tapeworm Moniezia expansa.

The tapeworm Moniezia expansa contains an extremely abundant cytoplasmic lipid-binding protein (LBP). It is a small protein consisting of 66 amino acids with a molecular mass of 7943 +/- 1.5 Da. The amino acid sequence has been established by Edman degradation and confirmed by PCR analysis. The Moniezia LBP shows no sequence similarity with any previously described binding protein, but does show similarity with antigen B from Echinococcus glanulosus and Echinococcus multilocularis and with Taenia crassiceps antigen. The predicted structure for Moniezia LBP shows four helices and a putative tyrosine kinase site on the loop between helix 1 and 2. Each of the four helices has a well defined hydrophobic face. Studies with fluorescent probes suggest a single hydrophobic binding site. Results indicate that the single tryptophan residue in the molecule (Trp41) is involved in ligand binding, and calculation of the Stern-Volmer quenching constant shows that Trp41 is in a relatively hydrophobic environment.

[1]  J. Lakowicz,et al.  Quenching of protein fluorescence by oxygen. Detection of structural fluctuations in proteins on the nanosecond time scale. , 1973, Biochemistry.

[2]  C A Ghiron,et al.  Exposure of tryptophanyl residues in proteins. Quantitative determination by fluorescence quenching studies. , 1976, Biochemistry.

[3]  J. Sedmak,et al.  A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250. , 1977, Analytical biochemistry.

[4]  T. Gomi,et al.  Inactivation of rat liver S-adenosylhomocysteinase by iodoacetamide. , 1982, Biochemistry.

[5]  M. Eftink,et al.  Indole fluorescence quenching studies on proteins and model systems: use of the inefficient quencher succinimide , 1984 .

[6]  S. Bernhard,et al.  Mechanism of transfer of reduced nicotinamide adenine dinucleotide among dehydrogenases. , 1985, Biochemistry.

[7]  Robert B. Macgregor,et al.  Estimation of the polarity of the protein interior by optical spectroscopy , 1986, Nature.

[8]  N. C. Price,et al.  Denaturation and renaturation of the monomeric phosphoglycerate mutase from Schizosaccharomyces pombe. , 1987, The Biochemical journal.

[9]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[10]  A. Bairoch.  PROSITE: a dictionary of sites and patterns in proteins. , 1991, Nucleic acids research.

[11]  J. Veerkamp,et al.  Structural and functional features of different types of cytoplasmic fatty acid-binding proteins. , 1991, Biochimica et biophysica acta.

[12]  D. McManus,et al.  A protein secreted in vivo by Echinococcus granulosus inhibits elastase activity and neutrophil chemotaxis. , 1991, Molecular and biochemical parasitology.

[13]  G. Griffiths,et al.  A 14-kDa Schistosoma mansoni polypeptide is homologous to a gene family of fatty acid binding proteins. , 1991, The Journal of biological chemistry.

[14]  M. Garcia-Blanco,et al.  Fasciola hepatica: molecular cloning, nucleotide sequence, and expression of a gene encoding a polypeptide homologous to a Schistosoma mansoni fatty acid-binding protein. , 1992, Experimental parasitology.

[15]  J. Veerkamp,et al.  Fatty acid-binding proteins: structural and functional diversity. , 1992, Biochemical Society transactions.

[16]  D. Haldar,et al.  Regulation of mitochondrial and microsomal phospholipid synthesis by liver fatty acid-binding protein. , 1992, The Journal of biological chemistry.

[17]  M C Peitsch,et al.  A 3-D model for the CD40 ligand predicts that it is a compact trimer similar to the tumor necrosis factors. , 1993, International immunology.

[18]  A. Esteves,et al.  A developmentally regulated gene of Echinococcus granulosus codes for a 15.5-kilodalton polypeptide related to fatty acid binding proteins. , 1993, Molecular and biochemical parasitology.

[19]  J. Gordon,et al.  Rat intestinal fatty acid binding protein. A model system for analyzing the forces that can bind fatty acids to proteins. , 1993, The Journal of biological chemistry.

[20]  D. Pritchard,et al.  A PCR strategy for the isolation of glutathione S-transferases (GSTs) from nematodes. , 1994, International journal for parasitology.

[21]  D. Zarlenga,et al.  A Taenia crassiceps cDNA sequence encoding a putative immunodiagnostic antigen for bovine cysticercosis. , 1994, Molecular and biochemical parasitology.

[22]  M. Frosch,et al.  Sequence heterogeneity of the echinococcal antigen B. , 1994, Molecular and biochemical parasitology.

[23]  D. McManus,et al.  Gene cloning, overproduction and purification of a functionally active cytoplasmic fatty acid-binding protein (Sj-FABPC) from the human blood fluke Schistosoma japonicum. , 1994, Gene.

[24]  C Geourjon,et al.  SOPM: a self-optimized method for protein secondary structure prediction. , 1994, Protein engineering.

[25]  N. C. Price,et al.  The ABA-1 allergen of the parasitic nematode Ascaris suum: fatty acid and retinoid binding function and structural characterization. , 1995, Biochemistry.

[26]  J. Barrett,et al.  A novel lipid-binding protein from the cestode Moniezia expansa. , 1995, The Biochemical journal.

[27]  N. C. Price,et al.  The DvA-1 Polyprotein of the Parasitic Nematode Dictyocaulus viviparus , 1995, The Journal of Biological Chemistry.

[28]  Christophe Geourjon,et al.  SOPMA: significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments , 1995, Comput. Appl. Biosci..

[29]  J. Allen,et al.  The gp15/400 polyprotein antigen of Brugia malayi binds fatty acids and retinoids. , 1995, Molecular and biochemical parasitology.

[30]  M C Peitsch,et al.  ProMod and Swiss-Model: Internet-based tools for automated comparative protein modelling. , 1996, Biochemical Society transactions.

[31]  Richard P. Haugland,et al.  Handbook of fluorescent probes and research chemicals , 1996 .

[32]  J. Beauchamp,et al.  Secretion of a Novel, Developmentally Regulated Fatty Acid-binding Protein into the Perivitelline Fluid of the Parasitic Nematode, Ascaris suum* , 1997, The Journal of Biological Chemistry.

[33]  P. Smooker,et al.  Isolation, cloning, and expression of fatty-acid binding proteins from Fasciola gigantica. , 1997, Experimental parasitology.