论文信息 - High-resolution optical coherence microscopy for high-speed, in vivo cellular imaging.

High-resolution optical coherence microscopy for high-speed, in vivo cellular imaging.

Optical coherence microscopy (OCM) is demonstrated with a high-speed, broadband, reflective-grating phase modulator and a femtosecond Ti:Al2O3 laser. The novel system design permits high-resolution OCM imaging in a new operating regime in which a short coherence gate is used to relax the requirement for high-numerical-aperture confocal axial sectioning. In vivo cellular imaging is demonstrated in the Xenopus laevis tadpole and in human skin with a 3-microm coherence gate and a 30-microm confocal gate. The ability to achieve cellular imaging with a lower numerical aperture should facilitate the development of miniaturized probes for in vivo imaging applications.

[1] J. Fujimoto,et al. In vivo ultrahigh-resolution optical coherence tomography. , 1999, Optics letters.

[2] J. Fujimoto,et al. Optical coherence microscopy in scattering media. , 1994, Optics letters.

[3] J. Izatt,et al. Optical coherence microscopy in gastrointestinal tissues , 1996, Summaries of papers presented at the Conference on Lasers and Electro-Optics.

[4] D. Sampson,et al. Achromatic optical phase shifter-modulator. , 2001, Optics letters.

[5] B. Lemoff,et al. Quintic-phase-limited, spatially uniform expansion and recompression of ultrashort optical pulses. , 1993, Optics letters.

[6] J. Fujimoto,et al. High-speed phase- and group-delay scanning with a grating-based phase control delay line. , 1997, Optics letters.

[7] J G Fujimoto,et al. Sub-two-cycle pulses from a Kerr-lens mode-locked Ti:sapphire laser. , 1999, Optics letters.

[8] J. Fujimoto,et al. Optical Coherence Tomography , 1991 .

[9] Wolfgang Rudolph,et al. Comparative study of confocal and heterodyne microscopy for imaging through scattering media , 1996 .

[10] R. Webb,et al. Video-rate confocal scanning laser microscope for imaging human tissues in vivo. , 1999, Applied optics.

[11] R. Webb,et al. In vivo confocal scanning laser microscopy of human skin: melanin provides strong contrast. , 1995, The Journal of investigative dermatology.