pH-switchable electrochemical sensing platform based on chitosan-reduced graphene oxide/concanavalin a layer for assay of glucose and urea.

A facile and effective electrochemical sensing platform for the detection of glucose and urea in one sample without separation was developed using chitosan-reduced graphene oxide (CS-rGO)/concanavalin A (Con A) as a sensing layer. The CS-rGO/Con A with pH-dependent surface net charges exhibited pH-switchable response to negatively charged Fe(CN)6(3-). The principle for glucose and urea detection was essentially based on in situ pH-switchable enzyme-catalyzed reaction in which the oxidation of glucose catalyzed by glucose oxidase or the hydrolyzation of urea catalyzed by urease resulted in a pH change of electrolyte solution to give different electrochemical responses toward Fe(CN)6(3-). It was verified by cyclic voltammograms, differential pulse voltammograms, and electrochemical impedance spectroscopy. The resistance to charge transfer or amperometric current changed proportionally toward glucose concentration from 1.0 to 10.0 mM and urea concentration from 1.0 to 7.0 mM. On the basis of human serum experiments, the sensing platform was proved to be suitable for simultaneous assay of glucose and urea in a practical biosystem. This work not only gives a way to detect glucose and urea in one sample without separation but also provides a potential strategy for the detection of nonelectroactive species based on the enzyme-catalyzed reaction and pH-switchable biosensor.

[1]  Abdullah M. Asiri,et al.  Ultrathin graphitic carbon nitride nanosheets: a low-cost, green, and highly efficient electrocatalyst toward the reduction of hydrogen peroxide and its glucose biosensing application. , 2013, Nanoscale.

[2]  V. Bezrodnyi,et al.  Investigations of photophysical and generation properties of active elements based on dyes in aliphatic polyurethane matrix , 2013, Journal of Polymer Research.

[3]  Ning Wang,et al.  Detection of glucose based on bimetallic PtCu nanochains modified electrodes. , 2013, Analytical chemistry.

[4]  Li Wang,et al.  A novel bi-protein bio-interphase of cytochrome c and glucose oxidase: Electron transfer and electrocatalysis , 2013 .

[5]  Jianding Qiu,et al.  "On-off" switchable electrochemical affinity nanobiosensor based on graphene oxide for ultrasensitive glucose sensing. , 2013, Biosensors & bioelectronics.

[6]  Rong Zhang,et al.  Highly sensitive glucose sensor based on pt nanoparticle/polyaniline hydrogel heterostructures. , 2013, ACS nano.

[7]  Hetong Qi,et al.  Anion-exchange-based amperometric assay for heparin using polyimidazolium as synthetic receptor. , 2013, Analytical chemistry.

[8]  S. F. D’souza,et al.  Immobilization of the urease on eggshell membrane and its application in biosensor. , 2013, Materials science & engineering. C, Materials for biological applications.

[9]  M. Musiani,et al.  Electrochemical reduction of nitrate and nitrite in alkaline media at CuNi alloy electrodes , 2013 .

[10]  Xiaoyun Qin,et al.  Ni foam: a novel three-dimensional porous sensing platform for sensitive and selective nonenzymatic glucose detection. , 2013, The Analyst.

[11]  Abdullah M. Asiri,et al.  Facile synthesis of novel Ni(II)-based metal-organic coordination polymer nanoparticle/reduced graphene oxide nanocomposites and their application for highly sensitive and selective nonenzymatic glucose sensing. , 2013, The Analyst.

[12]  Javier Gonzalo-Ruiz,et al.  Iridium oxide pH sensor for biomedical applications. Case urea-urease in real urine samples. , 2013, Biosensors & bioelectronics.

[13]  Hongyun Liu,et al.  An on–off biosensor based on multistimuli-responsive polymer films with a binary architecture and bioelectrocatalysis , 2012 .

[14]  Zong Dai,et al.  Attomolar determination of coumaphos by electrochemical displacement immunoassay coupled with oligonucleotide sensing. , 2012, Analytical chemistry.

[15]  Min Wei,et al.  Temperature-controlled electrochemical switch based on layered double hydroxide/poly(N-isopropylacrylamide) ultrathin films fabricated via layer-by-layer assembly. , 2012, Langmuir : the ACS journal of surfaces and colloids.

[16]  Haoqing Hou,et al.  Architecture of Fe3O4–graphene oxide nanocomposite and its application as a platform for amino acid biosensing , 2012 .

[17]  Abdullah M. Asiri,et al.  A simple route for preparation of highly stable CuO nanoparticles for nonenzymatic glucose detection , 2012 .

[18]  Xuping Sun,et al.  Ag nanoparticles decorated polyaniline nanofibers: synthesis, characterization, and applications toward catalytic reduction of 4-nitrophenol and electrochemical detection of H2O2 and glucose , 2012 .

[19]  Abdullah M. Asiri,et al.  Synthesis of Ag nanoparticle-decorated 2,4,6-tris(2-pyridyl)-1,3,5-triazine nanobelts and their application for H2O2 and glucose detection. , 2012, The Analyst.

[20]  Liang Su,et al.  Pt nanoflower/polyaniline composite nanofibers based urea biosensor. , 2011, Biosensors & bioelectronics.

[21]  Longwei Yin,et al.  Platinum nanoparticle modified polyaniline-functionalized boron nitride nanotubes for amperometric glucose enzyme biosensor. , 2011, ACS applied materials & interfaces.

[22]  Xinxia Cai,et al.  A bifunctional biosensor for subcutaneous glucose monitoring by reverse iontophoresis , 2011 .

[23]  Xuping Sun,et al.  Synthesis of functional SiO₂-coated graphene oxide nanosheets decorated with Ag nanoparticles for H₂O₂ and glucose detection. , 2011, Biosensors & bioelectronics.

[24]  Lei Wang,et al.  Self-assembled graphene platelet-glucose oxidase nanostructures for glucose biosensing. , 2011, Biosensors & bioelectronics.

[25]  Xiuhua Zhang,et al.  Direct electrochemistry of glucose oxidase and biosensing for glucose based on boron-doped carbon-coated nickel modified electrode. , 2011, Biosensors & bioelectronics.

[26]  Xuping Sun,et al.  Mesoporous carbon microparticles as a novel fluorescent sensing platform for thrombin detection. , 2011, Biosensors & bioelectronics.

[27]  N. R. Kamini,et al.  Amperometric electrode for determination of urea using electrodeposited rhodium and immobilized urease , 2011 .

[28]  Jun‐Jie Zhu,et al.  Fabrication of Graphene–Quantum Dots Composites for Sensitive Electrogenerated Chemiluminescence Immunosensing , 2011 .

[29]  Kun Wang,et al.  Enhanced direct electrochemistry of glucose oxidase and biosensing for glucose via synergy effect of graphene and CdS nanocrystals. , 2011, Biosensors & bioelectronics.

[30]  N. Hu,et al.  pH-controllable bioelectrocatalysis of glucose by glucose oxidase loaded in weak polyelectrolyte layer-by-layer films with ferrocene derivative as mediator , 2010 .

[31]  Huiqin Yao,et al.  pH-Controllable on-off bioelectrocatalysis of bienzyme layer-by-layer films assembled by concanavalin A and glucoenzymes with an electroactive mediator. , 2010, Journal of Physical Chemistry B.

[32]  Shaoling Song,et al.  pH-Controllable bioelectrocatalysis based on "on-off" switching redox property of electroactive probes for spin-assembled layer-by-layer films containing branched poly(ethyleneimine). , 2010, The journal of physical chemistry. B.

[33]  Huiqin Yao,et al.  pH-switchable bioelectrocatalysis of hydrogen peroxide on layer-by-layer films assembled by concanavalin A and horseradish peroxidase with electroactive mediator in solution. , 2010, The journal of physical chemistry. B.

[34]  Huafeng Yang,et al.  Graphene/AuNPs/chitosan nanocomposites film for glucose biosensing. , 2010, Biosensors & bioelectronics.

[35]  Jun Liu,et al.  Glucose oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing. , 2009, Biosensors & bioelectronics.

[36]  Huiqin Yao,et al.  pH-Sensitive "on-off" switching behavior of layer-by-layer films assembled by concanavalin A and dextran toward electroactive probes and its application in bioelectrocatalysis. , 2009, The journal of physical chemistry. B.

[37]  Jian Zhou,et al.  Switchable electrode controlled by Boolean logic gates using enzymes as input signals. , 2009, Bioelectrochemistry.

[38]  Shusheng Zhang,et al.  A simple strategy for one-step construction of bienzyme biosensor by in-situ formation of biocomposite film through electrodeposition. , 2009, Biosensors & bioelectronics.

[39]  M. Tarlov,et al.  Selective binding of RNase B glycoforms by polydopamine-immobilized concanavalin A. , 2009, Analytical chemistry.

[40]  Xihong Guo,et al.  Increment of Density of Au Nanoparticles Deposited in Situ within Layer-by-Layer Films and Its Enhancement on the Electrochemistry of Ferrocenecarboxylic Acid and Bioelectrocatalysis , 2009 .

[41]  Wensheng Yang,et al.  Direct electrochemistry of glucose oxidase on a graphite nanosheet–Nafion composite film modified electrode , 2009 .

[42]  Ping Wu,et al.  Detection of glucose based on direct electron transfer reaction of glucose oxidase immobilized on highly ordered polyaniline nanotubes. , 2009, Analytical chemistry.

[43]  Fenghua Li,et al.  Carbon nanotube/gold nanoparticles/polyethylenimine-functionalized ionic liquid thin film composites for glucose biosensing. , 2008, Biosensors & bioelectronics.

[44]  Zhi‐Kang Xu,et al.  Chitosan-modified poly(acrylonitrile-co-acrylic acid) nanofibrous membranes for the immobilization of concanavalin A. , 2008, Biomacromolecules.

[45]  G. Wallace,et al.  Mechanically Strong, Electrically Conductive, and Biocompatible Graphene Paper , 2008 .

[46]  Zhenan Bao,et al.  Organic solar cells with solution-processed graphene transparent electrodes , 2008 .

[47]  Evgeny Katz,et al.  Polymer Brush-Modified Electrode with Switchable and Tunable Redox Activity for Bioelectronic Applications , 2008 .

[48]  Li Wang,et al.  A novel hydrogen peroxide sensor based on Ag nanoparticles electrodeposited on DNA-networks modified glassy carbon electrode , 2008 .

[49]  Joseph Wang.  Electrochemical glucose biosensors. , 2008, Chemical reviews.

[50]  Qin Xu,et al.  Ordered mesoporous polyaniline film as a new matrix for enzyme immobilization and biosensor construction. , 2007, Analytica chimica acta.

[51]  Scott S. Verbridge,et al.  Electromechanical Resonators from Graphene Sheets , 2007, Science.

[52]  Andre K. Geim,et al.  Electric Field Effect in Atomically Thin Carbon Films , 2004, Science.

[53]  J. Do,et al.  Urea biosensor based on PANi(urease)-Nafion/Au composite electrode. , 2004, Biosensors & bioelectronics.

[54]  Xiaoyun Qin,et al.  One-pot green synthesis of Ag nanoparticles-graphene nanocomposites and their applications in SERS, H2O2, and glucose sensing , 2012 .

[55]  R. Srivastava,et al.  Synthesis of mesostructured polyaniline using mixed surfactants, anionic sodium dodecylsulfate and non-ionic polymers and their applications in H2O2 and glucose sensing. , 2012, Colloids and surfaces. B, Biointerfaces.