Extracellular ATP functions in alleviating the decrease of PSII photochemistry caused by the infection of Xanthomonas campestris pv. phaseoli

Extracellular ATP (eATP) can function as a signalling molecule to regulate a wide range of cellular processes. This present work investigated the role of eATP in mediating the change of PSII (photosystem II) photochemistry of the tissues of Phaseolus vulgaris leaves infected with Xanthomonas campestris pv. phaseoli (Xcp). Infection of the leaves with Xcp caused a significant decrease in the the PSII maximal photochemical efficiency (Fv/Fm), the maximum quantum efficiency of PSII photochemistry at illumination (Fv′/Fm′), the PSII operating efficiency (ΦPSII), the rate of non-cyclic electron transport through PSII (ETR), photochemical quenching (qP), and eATP level in the tissues of the infected leaves. At the same time, the levels of non-photochemical quenching (qN) and the quantum yield of regulated energy dissipation of PSII (Y(NPQ)) were significantly increased. Application of exogenous ATP at 0·2 mm to uninfected leaves had no significant effect on any of the chlorophyll fluorescence parameters being measured. However, in the tissues of infected leaves, the application of exogenous ATP alleviated the decreases of the Fv/Fm, Fv′/Fm′, ΦPSII, ETR, qP and eATP level, and also abolished the increases of qN and Y(NPQ). These results suggest that the change of PSII photochemistry by pathogen infection could be mediated by eATP.

[1]  S. Delrot,et al.  An update on sugar transport and signalling in grapevine. , 2014, Journal of experimental botany.

[2]  Mari L. Salmi,et al.  Apyrase Suppression Raises Extracellular ATP Levels and Induces Gene Expression and Cell Wall Changes Characteristic of Stress Responses1[C][W][OPEN] , 2014, Plant Physiology.

[3]  G. Stacey,et al.  Identification of a Plant Receptor for Extracellular ATP , 2014, Science.

[4]  L. Guidi,et al.  Image changes in chlorophyll fluorescence of cucumber leaves in response to iron deficiency and resupply , 2013 .

[5]  É. Hideg,et al.  Salicylic acid may indirectly influence the photosynthetic electron transport. , 2012, Journal of plant physiology.

[6]  Jian Sun,et al.  An ATP signalling pathway in plant cells: extracellular ATP triggers programmed cell death in Populus euphratica. , 2012, Plant, cell & environment.

[7]  L. Herrera-Estrella,et al.  How Plants Sense Wounds: Damaged-Self Recognition Is Based on Plant-Derived Elicitors and Induces Octadecanoid Signaling , 2012, PloS one.

[8]  R. Fields,et al.  Imaging Single Photons and Intrinsic Optical Signals for Studies of Vesicular and Non-Vesicular ATP Release from Axons , 2011, Front. Neuroanat..

[9]  Á. Szepesi,et al.  Salicylic acid treatment via the rooting medium interferes with stomatal response, CO2 fixation rate and carbohydrate metabolism in tomato, and decreases harmful effects of subsequent salt stress. , 2011, Plant biology.

[10]  Alan M. Jones,et al.  Extracellular ATP signaling in plants. , 2010, Trends in cell biology.

[11]  Jian Wu,et al.  Both the stimulation and inhibition of root hair growth induced by extracellular nucleotides in Arabidopsis are mediated by nitric oxide and reactive oxygen species , 2010, Plant Molecular Biology.

[12]  K. Lindsey,et al.  The effects of extracellular adenosine 5′‐triphosphate on the tobacco proteome , 2010, Proteomics.

[13]  K. Lindsey,et al.  Extracellular ATP , 2009, Plant signaling & behavior.

[14]  K. Lindsey,et al.  Extracellular ATP is a regulator of pathogen defence in plants. , 2009, The Plant journal : for cell and molecular biology.

[15]  J. Davies,et al.  Plant extracellular ATP signalling by plasma membrane NADPH oxidase and Ca2+ channels. , 2009, The Plant journal : for cell and molecular biology.

[16]  M. Bolton.  Primary metabolism and plant defense--fuel for the fire. , 2009, Molecular plant-microbe interactions : MPMI.

[17]  U. Sonnewald,et al.  Infection of maize leaves with Ustilago maydis prevents establishment of C4 photosynthesis. , 2008, Journal of plant physiology.

[18]  David M Kramer,et al.  Determining the limitations and regulation of photosynthetic energy transduction in leaves. , 2007, Plant, cell & environment.

[19]  Ladislav Nedbal,et al.  Visualization of dynamics of plant-pathogen interaction by novel combination of chlorophyll fluorescence imaging and statistical analysis: differential effects of virulent and avirulent strains of P. syringae and of oxylipins on A. thaliana. , 2007, Journal of experimental botany.

[20]  W. Frommer.  Faculty Opinions recommendation of Extracellular ATP in plants. Visualization, localization, and analysis of physiological significance in growth and signaling. , 2007 .

[21]  H. Schwartz,et al.  Bacterial Diseases of Dry Edible Beans in the Central High Plains , 2007 .

[22]  Eric A. Barnard,et al.  International Union of Pharmacology LVIII: Update on the P2Y G Protein-Coupled Nucleotide Receptors: From Molecular Mechanisms and Pathophysiology to Therapy , 2006, Pharmacological Reviews.

[23]  B. Khakh,et al.  P2X receptors as cell-surface ATP sensors in health and disease , 2006, Nature.

[24]  Uwe Conrath,et al.  Systemic Acquired Resistance , 2006, Plant signaling & behavior.

[25]  T. Roitsch,et al.  Infection with virulent and avirulent P. syringae strains differentially affects photosynthesis and sink metabolism in Arabidopsis leaves , 2006, Planta.

[26]  P. Schulze-Lefert,et al.  Metabolic consequences of susceptibility and resistance (race-specific and broad-spectrum) in barley leaves challenged with powdery mildew. , 2006, Plant, cell & environment.

[27]  P. Mullineaux,et al.  Controlled levels of salicylic acid are required for optimal photosynthesis and redox homeostasis. , 2006, Journal of experimental botany.

[28]  Stephen C. Stout,et al.  Extracellular ATP Induces the Accumulation of Superoxide via NADPH Oxidases in Arabidopsis1 , 2006, Plant Physiology.

[29]  E. Weis,et al.  Photosynthesis and carbohydrate metabolism in tobacco leaves during an incompatible interaction with Phytophthora nicotianae , 2005 .

[30]  P. Mullineaux,et al.  The influence of the light environment and photosynthesis on oxidative signalling responses in plant-biotrophic pathogen interactions , 2005 .

[31]  R. Gilbertson,et al.  Genetic Diversity and Pathogenic Variation of Common Blight Bacteria (Xanthomonas campestris pv. phaseoli and X. campestris pv. phaseoli var. fuscans) Suggests Pathogen Coevolution with the Common Bean. , 2004, Phytopathology.

[32]  J. Salvage.  Image changes. , 2001, Nursing times.

[33]  J. Parker,et al.  Interplay of signaling pathways in plant disease resistance. , 2000, Trends in genetics : TIG.

[34]  K Maxwell,et al.  Chlorophyll fluorescence--a practical guide. , 2000, Journal of experimental botany.

[35]  Collin Thomas,et al.  A Role for Ectophosphatase in Xenobiotic Resistance , 2000, Plant Cell.

[36]  G. Guidotti,et al.  Glucose-dependent, cAMP-mediated ATP efflux from Saccharomyces cerevisiae. , 1997, Microbiology.

[37]  David Baker,et al.  Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipa , 2008 .

[38]  R. Parish,et al.  Extracellular ATP, ecto‐ATPase and calcium influx in Dictyostelium discoideum cells , 1980, FEBS letters.