Periodic operation of a continuous culture of Baker's yeast

The possibility of enhancing the biomass productivity of a continuous culture of Saccharomyces cerevisiae growing on a glucose‐limited medium is addressed. An unstructured Monod‐type model is first identified using steady‐state data. The culture is subjected to step changes in dilution rate, and it is seen that the Monod model is unable to predict even qualitatively the dynamic response of the culture. Incorporation of a time delay allows significant improvement in the transient fit. It is found that the culture has a time lag of about 3 h in adapting its growth rate. Cycling the dilution rate with a period of 3 h leads to substantial improvement in the average biomass productivity.

[1]  Walter Borzani,et al.  Response of a continuous anaerobic culture to periodic variation of the feeding mash concentration , 1976 .

[2]  W. Borzani,et al.  Responses of a continuous anaerobic culture to variations in the feeding rate. , 1977, Biotechnology and bioengineering.

[3]  J. E. Bailey,et al.  Cyclic operation of reaction systems: Effects of heat and mass transfer resistance , 1971 .

[4]  R. J. Hall,et al.  A mechanistic model of the aerobic growth of Saccharomyces cerevisiae , 1977, Biotechnology and bioengineering.

[5]  J. E. Bailey.  Necessary conditions for optimality in a general class of non-linear mixed boundary value control problems. , 1972 .

[6]  G Lyberatos,et al.  Effect of cycling on final mixed culture fate , 1987, Biotechnology and bioengineering.

[7]  Henry R. Bungay,et al.  Frequency response analysis of dilution rate effects on yeast growth , 1968 .

[8]  James E. Bailey,et al.  PERIODIC OPERATION OF CHEMICAL REACTORS: A REVIEW , 1974 .

[9]  H. Blanch,et al.  The effect of discontinuous feeding on ethanol production by saccharomyces cerevisiae , 1976 .

[10]  M. J. Bazin,et al.  Growth and composition of Escherichia coli subjected to square‐wave perturbations in nutrient supply: Effect of varying frequencies , 1979 .

[11]  P. Rogers,et al.  A chemostat study of ethanol inhibition , 1971, Biotechnology and bioengineering.

[12]  Anil Menawat,et al.  Singular optimal control strategy for a fed‐batch bioreactor: Numerical approach , 1987 .

[13]  J. Monod.  The Growth of Bacterial Cultures , 1949 .

[14]  Herbert E. Klei,et al.  Response of biological reactors to sinusoidal variations of substrate concentration , 1976 .