Enhancing the soft tissue seal around intraosseous transcutaneous amputation prostheses using silanized fibronectin titanium alloy

The success of intraosseous transcutaneous amputation prostheses (ITAP) relies on achieving a tight seal between the soft tissues and the implant in order to avoid infection. Fibronectin (Fn) may be silanized onto titanium alloy (Ti–6Al–4V) in order to promote soft-tissue attachment. The silanization process includes passivation with sulphuric acid, which alters surface characteristics. This study aimed to improve in vitro fibroblast adhesion to silanized fibronectin (SiFn) titanium alloy by omitting the passivation stage. Additionally, the study assessed the effects of SiFn on in vivo dermal attachment, comparing the results with adsorbed Fn, hydroxyapatite (HA), Fn adsorbed onto HA (HAFn) and uncoated controls. Surface topography was assessed using scanning electron microscopy, profilometry and contact angle measurement. Anti-vinculin antibodies were used to immunolocalize fibroblast adhesion sites. A histological assessment of soft-tissue attachment and cell alignment relative to implants in an in vivo ovine model was performed. Passivation resulted in rougher, more hydrophobic, microcracked surfaces and was associated with poorer fibroblast adhesion than unpassivated controls. SiFn and HAFn surfaces resulted in more favourable cell alignment in vivo, implying that dermal attachment was enhanced. These results suggest that SiFn and HAFn surfaces could be useful in optimizing the soft tissue seal around ITAP.

[1]  T. Webster,et al.  Nanotextured titanium surfaces for enhancing skin growth on transcutaneous osseointegrated devices. , 2010, Acta biomaterialia.

[2]  M. Textor,et al.  Bacterial biofilm formation versus mammalian cell growth on titanium-based mono- and bi-functional coating. , 2010, European cells & materials.

[3]  Y. Koyama,et al.  Cell adhesion and tissue response to hydroxyapatite nanocrystal-coated poly(L-lactic acid) fabric. , 2009, Journal of bioscience and bioengineering.

[4]  Toshio Igarashi,et al.  Influence of surface wettability on competitive protein adsorption and initial attachment of osteoblasts , 2009, Biomedical materials.

[5]  Yong-Dae Kwon,et al.  Attachment and growth behaviour of human gingival fibroblasts on titanium and zirconia ceramic surfaces , 2009, Biomedical materials.

[6]  Jan E Ellingsen,et al.  Titanium implant surface modification by cathodic reduction in hydrofluoric acid: surface characterization and in vivo performance. , 2009, Journal of biomedical materials research. Part A.

[7]  T. Lee,et al.  Effects of passivation treatments on titanium alloy with nanometric scale roughness and induced changes in fibroblast initial adhesion evaluated by a cytodetacher. , 2009, Journal of biomedical materials research. Part A.

[8]  J. Olerud,et al.  Models for the histologic study of the skin interface with percutaneous biomaterials , 2008, Biomedical materials.

[9]  W. H. Goldmann,et al.  Corrigendum to “Vinculin promotes cell spreading by mechanically coupling integrins to the cytoskeleton” [Exp. Cell Res. 231 (1997) 14–26] , 2008 .

[10]  R. Cabrini,et al.  Biodistribution of titanium dioxide from biologic compartments , 2008, Journal of materials science. Materials in medicine.

[11]  J. Granjeiro,et al.  Effect of roughness of zirconia and titanium on fibroblast adhesion. , 2008, Artificial organs.

[12]  G W Blunn,et al.  Fibronectin silanized titanium alloy: a bioinductive and durable coating to enhance fibroblast attachment in vitro. , 2007, Journal of biomedical materials research. Part A.

[13]  M. Dunbar,et al.  Fibroblastic interactions with high-porosity Ti-6Al-4V metal foam. , 2007, Journal of biomedical materials research. Part B, Applied biomaterials.

[14]  Martin Schuler,et al.  Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients. , 2007, Biomaterials.

[15]  T. Ohno,et al.  Fibronectin–calcium phosphate composite layer on hydroxyapatite to enhance adhesion, cell spread and osteogenic differentiation of human mesenchymal stem cells in vitro , 2007, Biomedical materials.

[16]  H. Wendel,et al.  Photocoupling of fibronectin to titanium surfaces influences keratinocyte adhesion, pellicle formation and thrombogenicity. , 2007, Dental materials : official publication of the Academy of Dental Materials.

[17]  R. Narayanan,et al.  Phosphoric acid anodization of Ti–6Al–4V – Structural and corrosion aspects , 2007 .

[18]  Moon Suk Kim,et al.  Correlation of proliferation, morphology and biological responses of fibroblasts on LDPE with different surface wettability , 2007, Journal of biomaterials science. Polymer edition.

[19]  A. Goodship,et al.  Development of a soft tissue seal around bone-anchored transcutaneous amputation prostheses. , 2006, Biomaterials.

[20]  A. Goodship,et al.  Nature's answer to breaching the skin barrier: an innovative development for amputees , 2006, Journal of anatomy.

[21]  R. Jacobs,et al.  The neurophysiology of osseointegrated oral implants. A clinically underestimated aspect. , 2006, Journal of oral rehabilitation.

[22]  M. Barbosa,et al.  TiO2 type influences fibronectin adsorption , 2005, Journal of materials science. Materials in medicine.

[23]  G. Hildebrand,et al.  Comparison between RGD-peptide-modified titanium and borosilicate surfaces , 2005, Analytical and bioanalytical chemistry.

[24]  R. Glauser,et al.  The human bone-oxidized titanium implant interface: A light microscopic, scanning electron microscopic, back-scatter scanning electron microscopic, and energy-dispersive x-ray study of clinically retrieved dental implants. , 2005, Clinical implant dentistry and related research.

[25]  J. P. Li,et al.  A novel porous Ti6Al4V: characterization and cell attachment. , 2005, Journal of biomedical materials research. Part A.

[26]  P. Somasundaran,et al.  Thermal and chemical modification of titanium-aluminum-vanadium implant materials: effects on surface properties, glycoprotein adsorption, and MG63 cell attachment. , 2004, Biomaterials.

[27]  T. Groth,et al.  Surface properties of and cell adhesion onto allylamine-plasma-coated polyethylenterephtalat membranes. , 2003, Biomaterials.

[28]  J. Sullivan,et al.  Rehabilitation of the transfemoral amputee with an osseointegrated prosthesis: The United Kingdom experience , 2003, Prosthetics and orthotics international.

[29]  G. Whitesides,et al.  Cell shape provides global control of focal adhesion assembly. , 2003, Biochemical and biophysical research communications.

[30]  L. Sennerby,et al.  Histology of retrieved immediately and early loaded oxidized implants: light microscopic observations after 5 to 9 months of loading in the posterior mandible. , 2003, Clinical implant dentistry and related research.

[31]  J. Lindhe,et al.  Soft tissue reactions to plaque formation at implant abutments with different surface topography. An experimental study in dogs. , 2002, Journal of clinical periodontology.

[32]  A. Wennerberg,et al.  The mucosal attachment to titanium implants with different surface characteristics: an experimental study in dogs. , 2002, Journal of clinical periodontology.

[33]  L J Marks,et al.  Science, medicine, and the future: Artificial limbs. , 2001, BMJ.

[34]  Y. Oshida,et al.  Surface characterizations of variously treated titanium materials. , 2001, The International journal of oral & maxillofacial implants.

[35]  G. W. Davies,et al.  Surface topography and HA filler volume effect on primary human osteoblasts in vitro , 2000, Journal of materials science. Materials in medicine.

[36]  W. Wagner,et al.  Effects of hydroxylapatite coating crystallinity on biosolubility, cell attachment efficiency and proliferation in vitro. , 1999, Biomaterials.

[37]  P. Tresco,et al.  Relative importance of surface wettability and charged functional groups on NIH 3T3 fibroblast attachment, spreading, and cytoskeletal organization. , 1998, Journal of biomedical materials research.

[38]  M. McKee,et al.  Chemical modification of titanium surfaces for covalent attachment of biological molecules. , 1998, Journal of biomedical materials research.

[39]  D E Ingber,et al.  Vinculin promotes cell spreading by mechanically coupling integrins to the cytoskeleton. , 1997, Experimental cell research.

[40]  W. Nachtigall,et al.  Influence of the surface structure of titanium materials on the adhesion of fibroblasts. , 1996, Biomaterials.

[41]  A. Hall,et al.  The assembly of integrin adhesion complexes requires both extracellular matrix and intracellular rho/rac GTPases , 1995, The Journal of cell biology.

[42]  E. Roumanas,et al.  Auricular prostheses and osseointegrated implants: UCLA experience. , 1995, The Journal of prosthetic dentistry.

[43]  S. Raghavan,et al.  Gentamicin sulfate attachment and release from anodized Ti-6A1-4V orthopedic materials. , 1993, Journal of biomedical materials research.

[44]  D. Brunette,et al.  A light and electron microscopic study of the effects of surface topography on the behavior of cells attached to titanium-coated percutaneous implants. , 1991, Journal of biomedical materials research.

[45]  A. F. Recum,et al.  Applications and failure modes of percutaneous devices: A review , 1984 .

[46]  T. Groth,et al.  Surface modification of biomaterials to control adhesion of cells. , 2008, Clinical hemorheology and microcirculation.

[47]  G. Blunn,et al.  Sealing the skin barrier around transcutaneous implants: in vitro study of keratinocyte proliferation and adhesion in response to surface modifications of titanium alloy. , 2008, The Journal of bone and joint surgery. British volume.

[48]  L. Rasmusson,et al.  Osseointegration of oxidized and turned implants in circumferential bone defects with and without adjunctive therapies: an experimental study on BMP-2 and autogenous bone graft in the dog mandible. , 2007, International journal of oral and maxillofacial surgery.

[49]  Savio L-Y Woo,et al.  Cell orientation determines the alignment of cell-produced collagenous matrix. , 2003, Journal of biomechanics.

[50]  F. Grinnell.  Fibronectin and wound healing , 1984, Journal of cellular biochemistry.

[51]  P I Brånemark,et al.  A 15-year study of osseointegrated implants in the treatment of the edentulous jaw. , 1981, International journal of oral surgery.