Analysis of the killing effect of Mu ligts mutants on host cells

Infection of Escherichia coli with the mutant lig ts2 of bacteriophage Mu at a temperature nonpermissive for this mutant is lethal for the host cells. This effect is insensitive to phage immunity of the host cells, to inhibitors of protein synthesis and is not suppressed in trans in bacterial strains producing the Lig+ active protein. These data suggest that the killing effect of this mutant is different from the other kil functions identified in Mu [1].