Studies on the oxygen and carbon monoxide equilibria of human myoglobin.

Abstract 1. 1. A new spectrophotometric method of determining the oxygendissociation curves of Mb (and Hb) has been developed. 2. 2. The oxygen equilibrium of human crystallized Mb has been studied by this method. The effect of temperature, pH, ionic strength, and concentration of the pigment has been examined. 3. 3. The oxygen-dissociation curves of reconstituted Mb's and of Mb components Mb I and Mb II have also been investigated. 4. 4. The partition constant K = ( MbCO ) × p O 2 ( MbO 2 ) × p CO 2 , for human crystallized Mb has been determined.