Ultrasensitive and selective DNA detection based on nicking endonuclease assisted signal amplification and its application in cancer cell detection.

By significantly circumventing the typical problem of high background induced by excess hemin itself, an ultrasensitive DNA amplified detection method is developed based on nicking endonuclease (NEase) assisted strand scission cycle, which is further successfully applied in cancer cell detection.

[1]  Weihong Tan,et al.  Cell-specific internalization study of an aptamer from whole cell selection. , 2008, Chemistry.

[2]  Joshua E. Smith,et al.  Aptamer-conjugated nanoparticles for the collection and detection of multiple cancer cells. , 2007, Analytical chemistry.

[3]  C. Lieber,et al.  Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical Species , 2001, Science.

[4]  M. Estévez,et al.  Using aptamer-conjugated fluorescence resonance energy transfer nanoparticles for multiplexed cancer cell monitoring. , 2009, Analytical chemistry.

[5]  I. Willner,et al.  Ultrasensitive detection of DNA by the PCR-Induced generation of DNAzymes: the DNAzyme primer approach. , 2006, Chemical communications.

[6]  Itamar Willner,et al.  Cooperative multicomponent self-assembly of nucleic acid structures for the activation of DNAzyme cascades: a paradigm for DNA sensors and aptasensors. , 2009, Chemistry.

[7]  Itamar Willner,et al.  A virus spotlighted by an autonomous DNA machine. , 2006, Angewandte Chemie.

[8]  Wei Xu,et al.  Ultrasensitive and selective colorimetric DNA detection by nicking endonuclease assisted nanoparticle amplification. , 2009, Angewandte Chemie.

[9]  Itamar Willner,et al.  Detection of single-base DNA mutations by enzyme-amplified electronic transduction , 2001, Nature Biotechnology.

[10]  Itamar Willner,et al.  Amplified electrochemical detection of DNA through the aggregation of Au nanoparticles on electrodes and the incorporation of methylene blue into the DNA-crosslinked structure. , 2007, Chemical communications.

[11]  Maria Strømme,et al.  Multiplex detection of DNA sequences using the volume-amplified magnetic nanobead detection assay. , 2009, Analytical chemistry.

[12]  Juewen Liu,et al.  Functional DNA nanotechnology: emerging applications of DNAzymes and aptamers. , 2006, Current opinion in biotechnology.

[13]  Itamar Willner,et al.  A Fok I/DNA machine that duplicates its analyte gene sequence. , 2008, Journal of the American Chemical Society.

[14]  Weihong Tan,et al.  Aptamer-conjugated nanoparticles for selective collection and detection of cancer cells. , 2006, Analytical chemistry.

[15]  Sai Bi,et al.  Gold nanolabels for new enhanced chemiluminescence immunoassay of alpha-fetoprotein based on magnetic beads. , 2009, Chemistry.

[16]  Joshua E. Smith,et al.  Gold nanoparticle-based colorimetric assay for the direct detection of cancerous cells. , 2008, Analytical chemistry.

[17]  M. Ali,et al.  Rolling circle amplification: applications in nanotechnology and biodetection with functional nucleic acids. , 2008, Angewandte Chemie.

[18]  Itamar Willner,et al.  Autonomous fueled mechanical replication of nucleic acid templates for the amplified optical detection of DNA. , 2006, Angewandte Chemie.

[19]  Itamar Willner,et al.  A DNAzyme cascade for the amplified detection of Pb(2+) ions or L-histidine. , 2008, Chemical communications.

[20]  Xiangling Xiong,et al.  Nanoparticle-mediated IgE-receptor aggregation and signaling in RBL mast cells. , 2009, Journal of the American Chemical Society.

[21]  Weihong Tan,et al.  Cancer cell targeting using multiple aptamers conjugated on nanorods. , 2008, Analytical chemistry.

[22]  N. Dixon,et al.  Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies. , 2007, Angewandte Chemie.