Early post-mortem discrimination of water-holding capacity in pig longissimus muscle using new ultrasound method

Abstract Newly developed ultrasound equipment was tested for on-line determination of water-holding capacity (WHC) early post mortem (p.m.). The experiment was based on measurements of m. longissimus dorsi (LD) from 10 pigs, five of which were treated to result in pork with high WHC and five to result in pork with low WHC. Ultrasound measurements were carried out on the carcass of each pig every ten minutes from 15 to 85 min p.m. at three locations of the LD. Low field nuclear magnetic resonance (NMR) measurements were performed on excised samples simultaneously with the ultrasound measurements and at 24 h p.m. Drip loss was measured by Honikel's bag method. The ultrasound experiment demonstrated that classification of meat with high and low WHC was possible, but only at 85 min p.m., and only by the measurements obtained at the posterior end of the LD. NMR measurements underlined that there are structural differences in meat influenced by pre-slaughter treatment prior to 85 min p.m. However, meat properties that determine WHC were apparently not expressed sufficiently to be registered by the applied pragmatic handheld ultrasound equipment before 85 min p.m.

[1]  B. R. Thane,et al.  Principles of ultrasound and measurement of intramuscular fat. , 1992, Journal of animal science.

[2]  R. G. Kauffman,et al.  The effectiveness of examining early post-mortem musculature to predict ultimate pork quality. , 1993, Meat science.

[3]  G. Offer,et al.  Modelling of the formation of pale, soft and exudative meat: Effects of chilling regime and rate and extent of glycolysis. , 1991, Meat science.

[4]  J. Trinick,et al.  On the mechanism of water holding in meat: The swelling and shrinking of myofibrils. , 1983, Meat science.

[5]  J. Wismer‐Pedersen QUALITY OF PORK IN RELATION TO RATE OF pH CHANGE POST MORTEM , 1959 .

[6]  B. Hills,et al.  Magnetic resonance imaging in food science , 1998 .

[7]  J C Forrest,et al.  Development of technology for the early post mortem prediction of water holding capacity and drip loss in fresh pork. , 2000, Meat science.

[8]  R. Lawrie,et al.  The effect of pre-treatment with various drugs on post-mortem glycolysis and the onset of rigor mortis in rabbit skeletal muscle. , 1962, Journal of comparative pathology.

[9]  S B Engelsen,et al.  Prediction of water-holding capacity and composition of porcine meat by comparative spectroscopy. , 2000, Meat science.

[10]  G R Trout,et al.  Techniques for measuring water-binding capacity in muscle foods-A review of methodology. , 1988, Meat science.

[11]  M. V. Van Oeckel,et al.  Comparison of different methods for measuring water holding capacity and juiciness of pork versus on-line screening methods. , 1999, Meat science.

[12]  Søren Balling Engelsen,et al.  Pre-rigor conditions in beef under varying temperature- and pH-falls studied with rigometer, NMR and NIR , 2000 .

[13]  W. M. Robertson,et al.  A novel approach to grading pork carcasses: computer vision and ultrasound. , 2003, Meat science.

[14]  M. Ruusunen,et al.  Pork Quality: Genetic and Metabolic Factors. , 1993 .

[15]  H. J. Andersen,et al.  Physiological and structural events post mortem of importance for drip loss in pork. , 2002, Meat science.

[16]  R. Bro,et al.  Towards rapid and unique curve resolution of low-field NMR relaxation data: trilinear SLICING versus two-dimensional curve fitting. , 2002, Journal of magnetic resonance.

[17]  H. J. Andersen,et al.  Origin of multiexponential T(2) relaxation in muscle myowater. , 2001, Journal of agricultural and food chemistry.

[18]  D E Bray,et al.  Measuring intramuscular fat in beef with ultrasonic frequency analysis. , 1994, Journal of animal science.

[19]  H. J. Andersen,et al.  Comparative study of low-field NMR relaxation measurements and two traditional methods in the determination of water holding capacity of pork. , 2001, Meat Science.

[20]  S. Bañón,et al.  Objective assessment of pork quality. , 1994, Meat science.

[21]  Hanne Christine Bertram,et al.  Relationship between meat structure, water mobility, and distribution: a low-field nuclear magnetic resonance study. , 2002, Journal of agricultural and food chemistry.

[22]  H J Swatland,et al.  A review of the relationships of pH with physical aspects of pork quality. , 1988, Meat science.

[23]  S. Engelsen,et al.  NMR-cooking: monitoring the changes in meat during cooking by low-field 1H-NMR , 2002 .

[24]  A. Karlsson,et al.  Control of post mortem pH decrease in pig muscles: experimental design and testing of animal models. , 2000, Meat science.

[25]  K. Honikel,et al.  Reference methods for the assessment of physical characteristics of meat. , 1998, Meat science.

[26]  F. Capozzi,et al.  Relationships between (1)H NMR relaxation data and some technological parameters of meat: a chemometric approach. , 2000, Journal of magnetic resonance.

[27]  H. Busk,et al.  On-line pork carcass grading with the Autofom ultrasound system. , 1998, Journal of animal science.

[28]  H. J. Andersen,et al.  pH Dependence of the Progression in NMR T2 Relaxation Times in Post-mortem Muscle , 2003 .

[29]  H. Hotelling Analysis of a complex of statistical variables into principal components. , 1933 .

[30]  H. J. Andersen,et al.  Early prediction of water-holding capacity in meat by multivariate vibrational spectroscopy. , 2003, Meat science.

[31]  S. Brown,et al.  The relationships between initial pH, reflectance and exudation in pig muscle. , 1987, Meat science.