Mammalian glucocorticoid receptor derivatives enhance transcription in yeast.

In mammalian cells, the glucocorticoid receptor binds specifically to glucocorticoid response element (GRE) DNA sequences and enhances transcription from linked promoters. It is shown here that derivatives of the glucocorticoid receptor also enhance transcription when expressed in yeast. Receptor-mediated enhancement in yeast was observed in fusions of GRE sequences to the yeast cytochrome c1 (CYC1) promoter; the CYC1 upstream activator sequences were not essential, since enhancement was observed in fusions of GREs to mutant CYC1 promoters retaining only the TATA region and transcription startpoints. It is concluded that the receptor operates by a common, highly conserved mechanism in yeast and mammalian cells.

[1]  K. Yamamoto,et al.  Signal transduction and transcriptional regulation by glucocorticoid receptor-LexA fusion proteins. , 1988, Science.

[2]  P. Sharp,et al.  A yeast and a human CCAAT-binding protein have heterologous subunits that are functionally interchangeable , 1988, Cell.

[3]  L. Guarente.  UASs and enhancers: Common mechanism of transcriptional activation in yeast and mammals , 1988, Cell.

[4]  P. Chambon,et al.  The yeast UASG is a transcriptional enhancer in human hela cells in the presence of the GAL4 trans-activator , 1988, Cell.

[5]  M. Ptashne,et al.  GAL4 activates gene expression in mammalian cells , 1988, Cell.

[6]  R. Brent,et al.  DNA-bound Fos proteins activate transcription in yeast , 1988, Cell.

[7]  T. Maniatis,et al.  Regulation of inducible and tissue-specific gene expression. , 1987, Science.

[8]  K. Yamamoto,et al.  Glucocorticoid receptor mutants that define a small region sufficient for enhancer activation. , 1987, Science.

[9]  L. Guarente,et al.  Yeast HAP1 activator competes with the factor RC2 for binding to the upstream activation site UAS1 of the CYC1 gene , 1987, Cell.

[10]  R. Evans,et al.  Colocalization of DNA-binding and transcriptional activation functions in the human glucocorticoid receptor , 1987, Cell.

[11]  B. Gloss,et al.  Cooperativity of glucocorticoid response elements located far upstream of the tyrosine aminotransferase gene , 1987, Cell.

[12]  R. Koski,et al.  Expression and secretion vectors for yeast. , 1987, Methods in enzymology.

[13]  K. Yamamoto,et al.  Glucocorticoid receptor mutants that are constitutive activators of transcriptional enhancement , 1987, Nature.

[14]  M. Montagu,et al.  Light-inducible and tissue-specific pea lhcp gene expression involves an upstream element combining enhancer- and silencer-like properties , 1986, Nature.

[15]  R. Evans,et al.  Functional domains of the human glucocorticoid receptor , 1986, Cell.

[16]  K. Yamamoto,et al.  Genetic complementation of a glucocorticoid receptor deficiency by expression of cloned receptor cDNA , 1986, Cell.

[17]  P. Gruss,et al.  The role of enhancers in the regulation of cell-type-specific transcriptional control , 1986 .

[18]  B. Magasanik,et al.  Transcription of glnA in E. coli is stimulated by activator bound to sites far from the promoter , 1986, Cell.

[19]  M. Garabedian,et al.  A tissue-specific transcription enhancer from the Drosophila yolk protein 1 gene , 1986, Cell.

[20]  M. Montagu,et al.  Light regulation of plant gene expression by an upstream enhancer-like element , 1985, Nature.

[21]  I. Herskowitz,et al.  A repressor (MATα2 product) and its operator control expression of a set of cell type specific genes in yeast , 1985, Cell.

[22]  B. Groner,et al.  Hormonal response region in the mouse mammary tumor virus long terminal repeat can be dissociated from the proviral promoter and has enhancer properties. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[23]  K. Yamamoto,et al.  Steroid receptor regulated transcription of specific genes and gene networks. , 1985, Annual review of genetics.

[24]  R. Brent,et al.  A bacterial repressor protein or a yeast transcriptional terminator can block upstream activation of a yeast gene , 1984, Nature.

[25]  H. Domdey,et al.  Lariat structures are in vivo intermediates in yeast pre-mRNA splicing , 1984, Cell.

[26]  G. Bitter,et al.  Expression of heterologous genes in Saccharomyces cerevisiae from vectors utilizing the glyceraldehyde-3-phosphate dehydrogenase gene promoter. , 1984, Gene.

[27]  L. Guarente,et al.  Upstream activation sites of the CYC1 gene of Saccharomyces cerevisiae are active when inverted but not when placed downstream of the "TATA box". , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[28]  Keith R. Yamamoto,et al.  Reversible and persistent changes in chromatin structure accompany activation of a glucocorticoid-dependent enhancer element , 1984, Cell.

[29]  R. Harrison,et al.  Characterization of a monoclonal antibody to the rat liver glucocorticoid receptor. , 1984, Endocrinology.

[30]  K. Yamamoto,et al.  Sequence-specific binding of glucocorticoid receptor to MTV DNA at sites within and upstream of the transcribed region , 1983, Cell.

[31]  M. Beato,et al.  The glucocorticoid receptor binds to defined nucleotide sequences near the promoter of mouse mammary tumour virus , 1983, Nature.

[32]  K. Yamamoto,et al.  DNA sequences bound specifically by glucocorticoid receptor in vitro render a heterologous promoter hormone responsive in vivo , 1983, Cell.

[33]  K. Murata,et al.  Transformation of intact yeast cells treated with alkali cations , 1983 .