Larvicidal, repellent and ovicidal activity of Calotropis gigantea against Culex gelidus, Culex tritaeniorhynchus (Diptera: Culicidae).

In this study, aqueous of Calotropis gigantea leaves were screened for larvicidal activity, mosquito repellent activity and ovicidal activity against Culex gelidus and C. tritaeniorhynchus mosquitoes. The extract doesn’t exhibit 100 % mortality against both the mosquito larvae. However, the extract showed dose dependent larvicidal activity with an motility rate of 86±1.42% (LC50=137.90) against C. gelidus and 94±1.31% (LC50=110.05) against C. tritaeniorhynchus. At 125, 250, 500 and 1000 ppm dose C. gigantea leaves extract exhibits complete protection from mosquito bite for 60, 90, 90 and 240 minutes against C. gelidus and 60, 90, 90 and 120 minutes respectively against C. tritaeniorhynchus. C. gigantea showed moderate ovicidal at 1000 ppm concentration against both mosquitoes eggs. Phytochemical analysis of the extract exhibited the presence of phenolic compounds, flavonoids, alkaloids, tannins, saponins, glycosides and phytosterols as major phytochemical groups. These results suggest that the leaves of C. gigantea have the potential to be used as a natural source for the development of new, safe, potential and eco-friendly insecticide for the control of C. gelidus and C. tritaeniorhynchus mosquitoes.

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