Comparative analysis of sodium‐dependent l‐glutamate transport of synaptosomal and astroglial membrane vesicles from mouse cortex

Uptake of [3H]l‐glutamate into membrane vesicles prepared from either mouse cortical astrocyte cultures or synaptosomes was found to be an electrogenic sodium‐ and potassium‐dependent transport process with saturable uptake kinetics. Pharmacological differences were revealed by using a variety of substrate analogues. l‐trans‐PDC inhibited the synaptosomal glutamate transport 2–4‐fold stronger than the astroglial uptake. The substrate analogues dl‐threo‐β‐hydroxy‐aspartate, dl‐aspartate‐β‐hydroxamate, l‐aspartate and d‐aspartate inhibited glutamate transport of astroglial and neuronal membrane vesicles in a distinctive manner, whereas d‐glutamate, quisqualate and dihydrokainate had no effect in either case. Immunoblotting and immunocytochemical labeling with antibodies against the rat brain glutamate transporter revealed the selective reaction of a band at about 75 kDa mol. wt. and a specific pattern of astrocyte immunostaining.

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