Acetyl-cholinesterase Enzyme Inhibitory Effect of Calophyllum species

Purpose: To search for new acetylcholinesterase enzyme inhibitors from Calopyllum species. Methods: Six stem bark extracts of Calophyllum inophyllum , C. soulattri , C. teysmannii , C. lowii , C. benjaminum and C. javanicum were subjected to anti-cholinesterase analysis against acetylcholinesterase (AChE) enzyme using Ellman’s method. Results: Most of the extracts showed promising inhibitory activity against AChE at concentrations of 100 μg/mL, with the methanol extract of C. inophyllum demonstrating the strongest inhibitory effect of 81.28 % followed by the methanol extract of C. benjaminum with 74.32 % inhibition. The methanol extracts of Calophyllum inophyllum and Calophyllum bejaminum also showed significant inhibitory activity towards the acetylcholinesterase (AChE) enzyme. Conclusion: Calophyllum species is capable of yielding potential lead compounds for the development for acetyl-cholinesterase enzyme inhibition drugs. Keywords: Calophyllum species, Acetylcholinesterase, Inhibitors

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