SARAMA: A Standalone Suite of Programs for the Complementarity Plot—A Graphical Structure Validation Tool for Proteins

Structure validation is a crucial component not only in protein crystallography but also in model quality estimation in homology modeling, protein design and de-novo structure prediction. Two key attributes of a correctly determined atomic model are optimal packing between side-chains and absence of destabilizing unbalanced electric fields within the interior of a protein molecule. The complementarity plot (CP) combines them in a single unified measure. CP has now been compiled into a user friendly validation package and made available as a standalone suite of programs in the public domain (http://www.saha.ac.in/biop/www/sarama.html). The application of CP in the detection of wrong rotamer assignment has been surveyed.