Lymphatic removal of fluids and particles in the mammalian lung.

The structure and distribution of pulmonary lymphatics and their permeability to fluids and particulate materials have been investigated in the lungs of rats following fixation by combined intratracheal and vascular perfusion. In such preparations, the lymphatics remain in a distended state, and a close relationship to other structural components of the pulmonary interstitium is maintained. They were identified in regions with an abundant amount of connective tissue, forming an eleborate plexus within the pleura, the interlobular septum, peribronchial and perivascular areas. Recent data have shown that water-soluble molecules and particulate matter are removed from the interstitium along the lymphatic capillary (initial lymphatics) segment. It is distinguished by attenuated endothelial cells with extensively overlapping cell margins which are easily separated. We have studied this segment of the lymphatic vascular system following intratracheal injections of colloidal particles (ferritin and carbon) to determine the structural features responsible for the transport of large molecules and particulate materials across the lymphatic endothelial wall in the lung. The results showed that the tracer particles cross the lymphatic endothelial wall via the clefts of intercellular junctions. While the tracer particles were observed within vesicles, the question of transport across the lymphatic endothelium via plasmalemmal vesicles is still not settled since the number and size of vesicles containing tracer particles also increased with time. Intravascular injected dextran was also localized within the clefts of intercellular junctions and plasmalemmal vesicles. The results obtained with intratracheal and intravascular injected tracer substances are consistent with those observed in lymphatic capillaries for other tissues. ImagesFIGURE 1. 1aFIGURE 1. 1bFIGURE 2.FIGURE 3.FIGURE 4.FIGURE 5.FIGURE 6.FIGURE 7.FIGURE 8.FIGURE 9.FIGURE 10.FIGURE 11.FIGURE 12.FIGURE 13.FIGURE 14.FIGURE 15.FIGURE 16.FIGURE 17.FIGURE 18.FIGURE 19.

[1]  J. Baert,et al.  Fine filaments in lymphatic endothelial cells , 1976, The Journal of cell biology.

[2]  J. Brain,et al.  Pathways of clearance in mouse lungs exposed to iron oxide aerosols , 1975, The Anatomical record.

[3]  A. Engeset,et al.  Studies on human peripheral lymph. I. Sampling method. , 1973, Lymphology.

[4]  L. Leak.  The transport of exogenous peroxidase across the blood-tissue-lymph interface. , 1972, Journal of ultrastructure research.

[5]  B. Morris.  The Cells of Lymph and Their Role in Immunological Reactions , 1972 .

[6]  N. Simionescu,et al.  DEXTRANS AND GLYCOGENS AS PARTICULATE TRACERS FOR STUDYING CAPILLARY PERMEABILITY , 1971, The Journal of cell biology.

[7]  L. Leak.  STUDIES ON THE PERMEABILITY OF LYMPHATIC CAPILLARIES , 1971, The Journal of cell biology.

[8]  J. Lauweryns.  The blood and lymphatic microcirculation of the lung. , 1971, Pathology annual.

[9]  L. Leak.  Electron microscopic observations on lymphatic capillaries and the structural components of the connective tissue-lymph interface. , 1970, Microvascular research.

[10]  J. M. Yoffey,et al.  Lymphatics, lymph and the lymphomyeloid complex , 1970 .

[11]  F. Courtice,et al.  Measurements of protein leakage in the acute and recovery stages of a thermal injury. , 1969, The Australian journal of experimental biology and medical science.

[12]  A C Guyton,et al.  Interstitial Fluid Pressure , 1968, Circulation research.

[13]  G. Lewis,et al.  Intracellular enzymes in local lymph as a measure of cellular injury , 1967, The Journal of physiology.

[14]  J. Hall,et al.  THE ULTRASTRUCTURE AND FUNCTION OF THE CELLS IN LYMPH FOLLOWING ANTIGENIC STIMULATION , 1967, The Journal of experimental medicine.

[15]  R. Blomstrand,et al.  ALKALINE PHOSPHATASE ACTIVITY IN HUMAN THORACIC DUCT LYMPH. , 1965, Acta chirurgica Scandinavica.

[16]  M. Karnovsky,et al.  A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron-microscopy , 1965 .

[17]  D. Trapnell.  THE PERIPHERAL LYMPHATICS OF THE LUNG. , 1963, The British journal of radiology.

[18]  H. S. Mayerson.  ON LYMPH AND LYMPHATICS. , 1963, Circulation.

[19]  H. S. Mayerson,et al.  Permeability of lymphatic vessels. , 1962, The American journal of physiology.

[20]  B. Morris,et al.  The flow and composition of lymph from the mammary gland in merino sheep. , 1961, Quarterly journal of experimental physiology and cognate medical sciences.

[21]  G. Grotte.  Passage of dextran molecules across the blood-lymph barrier. , 1956, Acta chirurgica Scandinavica. Supplementum.

[22]  F. Courtice,et al.  Physiological significance of lymph drainage of the serous cavities and lungs. , 1954, Physiological reviews.

[23]  T. F. Anderson,et al.  TECHNIQUES FOR THE PRESERVAATION OF THREE-DIMENSIONAL STRUCTURE IN PREPARING SPECIMENS FOR THE ELECTRON MICROSCOPE† , 1951 .

[24]  J. Bollman,et al.  Techniques for the collection of lymph from the liver, small intestine, or thoracic duct of the rat. , 1948, The Journal of laboratory and clinical medicine.

[25]  J. Bollman,et al.  Alkaline phosphatase in the intestinal lymph of the rat. , 1948, The Journal of biological chemistry.

[26]  C. K. Drinker,et al.  Absorption from the pulmonary alveoli. , 1947, Federation proceedings.