Controlling droplet incubation using close-packed plug flow.

Controlling droplet incubation is critical for droplet-based microfluidic applications; however, current techniques are either of limited precision or place strict limits on the incubation times that can be achieved. Here, we present a simple technique to control incubation time by exploiting close-packed plug flow. In contrast to other techniques, this technique is applicable to very short and very long incubation times.

[1]  Helen Song,et al.  Reactions in droplets in microfluidic channels. , 2006, Angewandte Chemie.

[2]  A. Abate,et al.  Ultrahigh-throughput screening in drop-based microfluidics for directed evolution , 2010, Proceedings of the National Academy of Sciences.

[3]  Andrew D Griffiths,et al.  Multi-step microfluidic droplet processing: kinetic analysis of an in vitro translated enzyme. , 2009, Lab on a chip.

[4]  Christoph A. Merten,et al.  Drop-based microfluidic devices for encapsulation of single cells. , 2008, Lab on a chip.

[5]  A. Griffiths,et al.  Reliable microfluidic on-chip incubation of droplets in delay-lines. , 2009, Lab on a chip.

[6]  A. Abate,et al.  High-throughput injection with microfluidics using picoinjectors , 2010, Proceedings of the National Academy of Sciences.

[7]  H. Stone,et al.  Hydrodynamic dispersion in shallow microchannels: the effect of cross-sectional shape. , 2006, Analytical chemistry.

[8]  Daniel Bratton,et al.  Development of quantitative cell-based enzyme assays in microdroplets. , 2008, Analytical chemistry.

[9]  Hsueh-Chia Chang,et al.  Marangoni effects of trace impurities on the motion of long gas bubbles in capillaries , 1990, Journal of Fluid Mechanics.

[10]  David A. Weitz,et al.  Valve-based flow focusing for drop formation , 2009 .

[11]  宁北芳,et al.  疟原虫var基因转换速率变化导致抗原变异[英]/Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A , 2005 .

[12]  H. Stone,et al.  Formation of dispersions using “flow focusing” in microchannels , 2003 .

[13]  Hsueh-Chia Chang,et al.  Transport of gas bubbles in capillaries , 1989 .

[14]  大房 健 基礎講座 電気泳動(Electrophoresis) , 2005 .

[15]  Olivier Harismendy,et al.  Microdroplet-based Pcr enrichment for large-scale targeted sequencing , 2016 .

[16]  Florian Hollfelder,et al.  The potential of microfluidic water-in-oil droplets in experimental biology. , 2009, Molecular bioSystems.

[17]  Magalie Faivre,et al.  Microfluidic flow focusing: Drop size and scaling in pressure versus flow‐rate‐driven pumping , 2005, Electrophoresis.

[18]  D A Weitz,et al.  Impact of inlet channel geometry on microfluidic drop formation. , 2009, Physical review. E, Statistical, nonlinear, and soft matter physics.

[19]  R. Rosenfeld Nature , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[20]  Armand Ajdari,et al.  Droplet Control for Microfluidics , 2005, Science.

[21]  Liang Li,et al.  Nanoliter microfluidic hybrid method for simultaneous screening and optimization validated with crystallization of membrane proteins , 2006, Proceedings of the National Academy of Sciences.

[22]  D. Weitz,et al.  Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity. , 2009, Lab on a chip.

[23]  N. Perrimon,et al.  Droplet microfluidic technology for single-cell high-throughput screening , 2009, Proceedings of the National Academy of Sciences.

[24]  H. Makse,et al.  A phase diagram for jammed matter , 2008, Nature.

[25]  B. R. Patton Solid State Physics: Solid State Physics , 2001 .

[26]  A. Sood Structural Ordering in Colloidal Suspensions , 1991 .