Classic and Contemporary Neural Tract-Tracing Techniques

Abstract Investigating the organization of nerve pathways is of fundamental interest in neurology and the neurosciences. In this chapter we draw attention to landmark events that have contributed to the scientific evolution of neuron tract tracing and cover in detail contemporary methods employed in experimental animals to trace nerve pathways. The basic anatomical tract-tracing methods, including the cellular mechanisms underlying tract tracer uptake and axonal transport, are described. Specific techniques and experimental applications involving tracers transported retrogradely, anterogradely, and transneuronally are reviewed. Finally, examples of combined neuroanatomical tract-tracing applications are discussed and illustrated.

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