In human motion analysis, bone motions are usually expressed relatively to anatomical reference frames. The anatomical reference frames are constructed thanks to the localization of bony landmarks during a static phase prior to the acquisitions. These landmarks are identified by means of palpation. Accurate comparison between subjects and studies implies good reproducibility and repeatability of the palpation process. However, all investigators don’t have a long experience in palpation. In this paper, the reproducibility and repeatability of palpation for junior investigators were measured. Results show worse reproducibility and repeatability than what is usually expected. These errors have particularly an influence on the definition of the reference frames of the arm. This study therefore emphasizes on the need of a specific training of operators working in a motion lab.