Fabrication of a photo-crosslinked gelatin hydrogel for preventing abdominal adhesion

Natural hydrogels are promising membranes used to prevent intra-abdominal adhesion formation. Currently, natural hydrogels such as chitosan-, gelatin- or hyaluronic acid-based hydrogels are utilized to prevent adhesion. However, their uncontrollable mechanical properties and quick degradation result in an unsatisfying short effect-time. In this study, a photocrosslinkable gelatin (GelMA) prepolymer was synthesized and developed for preventing intra-abdominal adhesion formation. Hydrogel membranes based on GelMA showed easy-handleability, non-toxic degradation and a long-lasting excellent barrier effect for up to 1 month. We have found that a 20% GelMA hydrogel membrane concentration could be employed to meet the requirements of excellent barrier effect, and the implantation of GelMA hydrogel membranes in rat abdominal cavities in experimental groups led to a considerable decrease in adhesion formation in comparison to the control group. The present study established the initial foundation for a novel and practical approach to prevent abdominal adhesion in surgery.

[1]  A. Di Spiezio Sardo,et al.  Prevention of intrauterine post-surgical adhesions in hysteroscopy. A systematic review. , 2016, European journal of obstetrics, gynecology, and reproductive biology.

[2]  W. Cui,et al.  Biomaterials for facial aging , 2016 .

[3]  W. Cui,et al.  Intradermal fillers for minimally invasive treatment of facial aging , 2016 .

[4]  W. Cui,et al.  An update review on recent skin fillers , 2016 .

[5]  Ali Khademhosseini,et al.  Photocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering , 2016, Advanced healthcare materials.

[6]  A. Khademhosseini,et al.  Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. , 2015, Biomaterials.

[7]  N. Bouvy,et al.  Postoperative abdominal adhesions and bowel obstruction. A survey among Dutch general practitioners , 2015, The European journal of general practice.

[8]  Fuyuki F Inagaki,et al.  Cell‐based therapy for preventing postoperative adhesion and promoting regeneration after hepatectomy , 2015, Journal of hepato-biliary-pancreatic sciences.

[9]  M. Diamond,et al.  The biology of adhesion formation in the peritoneal cavity. , 2014, Seminars in pediatric surgery.

[10]  A. Mikos,et al.  A factorial analysis of the combined effects of hydrogel fabrication parameters on the in vitro swelling and degradation of oligo(poly(ethylene glycol) fumarate) hydrogels. , 2014, Journal of biomedical materials research. Part A.

[11]  Lei Zhou,et al.  Cell-laden photocrosslinked GelMA–DexMA copolymer hydrogels with tunable mechanical properties for tissue engineering , 2014, Journal of Materials Science: Materials in Medicine.

[12]  P. Rutgeerts,et al.  Review article: anti‐adhesion therapies for inflammatory bowel disease , 2014, Alimentary pharmacology & therapeutics.

[13]  M. Stommel,et al.  Benefits and harms of adhesion barriers for abdominal surgery: a systematic review and meta-analysis , 2014, The Lancet.

[14]  J. Jeekel,et al.  Burden of adhesions in abdominal and pelvic surgery: systematic review and met-analysis , 2013, BMJ.

[15]  W. Cui,et al.  Prevention of peritendinous adhesions with electrospun ibuprofen-loaded poly(L-lactic acid)-polyethylene glycol fibrous membranes. , 2013, Tissue engineering. Part A.

[16]  Ali Khademhosseini,et al.  Functional Human Vascular Network Generated in Photocrosslinkable Gelatin Methacrylate Hydrogels , 2012, Advanced functional materials.

[17]  O. Mynbaev,et al.  Severe inflammatory reaction induced by peritoneal trauma is the key driving mechanism of postoperative adhesion formation , 2011, BMC surgery.

[18]  S. Thrumurthy,et al.  Unexpected outcome from Trousseau syndrome , 2011, BMC surgery.

[19]  Mark M. Melendez,et al.  Novel macromolecular crosslinking hydrogel to reduce intra-abdominal adhesions. , 2008, The Journal of surgical research.

[20]  Sheila MacNeil,et al.  Progress and opportunities for tissue-engineered skin , 2007, Nature.

[21]  A. Larentzakis,et al.  Acute mechanical bowel obstruction: clinical presentation, etiology, management and outcome. , 2007, World journal of gastroenterology.

[22]  M. H. Gil,et al.  Structural analysis of dextran-based hydrogels obtained chemoenzymatically. , 2006, Journal of biomedical materials research. Part B, Applied biomaterials.

[23]  S. Yuk,et al.  Tissue anti-adhesion potential of ibuprofen-loaded PLLA-PEG diblock copolymer films. , 2005, Biomaterials.

[24]  Thomas Zimmerman,et al.  Prevention of Postsurgery-Induced Abdominal Adhesions by Electrospun Bioabsorbable Nanofibrous Poly(lactide-co-glycolide)-Based Membranes , 2004, Annals of surgery.

[25]  H. van Goor,et al.  Pathophysiology of intra‐abdominal adhesion and abscess formation, and the effect of hyaluronan , 2003, The British journal of surgery.

[26]  K. Ulubayram,et al.  Cytotoxicity evaluation of gelatin sponges prepared with different cross-linking agents , 2002, Journal of biomaterials science. Polymer edition.

[27]  M Cornelissen,et al.  Structural and rheological properties of methacrylamide modified gelatin hydrogels. , 2000, Biomacromolecules.

[28]  Richard A. Harris,et al.  Comparison of Antiadhesive Treatments using an Objective Rat Model , 1999, The American surgeon.

[29]  R. Bronson,et al.  Prevention of tissue injury and postsurgical adhesions by precoating tissues with hyaluronic acid solutions. , 1995, The Journal of surgical research.

[30]  E. Goldberg,et al.  Prevention of postoperative abdominal adhesions by tissue precoating with polymer solutions. , 1993, The Journal of surgical research.