Retracted: A subfamily of RNA‐binding DEAD‐box proteins acts as an estrogen receptor α coactivator through the N‐terminal activation domain (AF‐1) with an RNA coactivator, SRA

One class of the nuclear receptor AF‐2 coactivator complexes contains the SRC‐1/TIF2 family, CBP/p300 and an RNA coactivator, SRA. We identified a subfamily of RNA‐binding DEAD‐box proteins (p72/p68) as a human estrogen receptor α (hERα) coactivator in the complex containing these factors. p72/p68 interacted with both the AD2 of any SRC‐1/TIF2 family protein and the hERα A/B domain, but not with any other nuclear receptor tested. p72/p68, TIF2 (SRC‐1) and SRA were co‐immunoprecipitated with estrogen‐bound hERα in MCF7 cells and in partially purified complexes associated with hERα from HeLa nuclear extracts. Estrogen induced co‐localization of p72 with hERα and TIF2 in the nucleus. The presence of p72/p68 potentiated the estrogen‐induced expression of the endogenous pS2 gene in MCF7 cells. In a transient expression assay, a combination of p72/p68 with SRA and one TIF2 brought an ultimate synergism to the estrogen‐induced transactivation of hERα. These findings indicate that p72/p68 acts as an ER subtype‐selective coactivator through ERα AF‐1 by associating with the coactivator complex to bind its AF‐2 through direct binding with SRA and the SRC‐1/TIF2 family proteins.

[1]  J. Arbiser,et al.  Phosphorylation by Mitogen-activated Protein Kinase Mediates the Hypoxia-induced Turnover of the TAL1/SCL Transcription Factor in Endothelial Cells* , 2002, The Journal of Biological Chemistry.

[2]  T. Kitamoto,et al.  p300 Mediates Functional Synergism between AF-1 and AF-2 of Estrogen Receptor α and β by Interacting Directly with the N-terminal A/B Domains* , 2000, The Journal of Biological Chemistry.

[3]  C. Glass,et al.  The coregulator exchange in transcriptional functions of nuclear receptors. , 2000, Genes & development.

[4]  D. Metzger,et al.  Purification and Identification of p68 RNA Helicase Acting as a Transcriptional Coactivator Specific for the Activation Function 1 of Human Estrogen Receptor α , 1999, Molecular and Cellular Biology.

[5]  B. Spiegelman,et al.  An adipogenic cofactor bound by the differentiation domain of PPARγ , 1999, The EMBO journal.

[6]  D. Aswad,et al.  Regulation of transcription by a protein methyltransferase. , 1999, Science.

[7]  K. Miyazono,et al.  Positive and Negative Modulation of Vitamin D Receptor Function by Transforming Growth Factor-β Signaling through Smad Proteins* , 1999, The Journal of Biological Chemistry.

[8]  Paul Tempst,et al.  Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex , 1999, Nature.

[9]  Neil J McKenna,et al.  A Steroid Receptor Coactivator, SRA, Functions as an RNA and Is Present in an SRC-1 Complex , 1999, Cell.

[10]  L. Freedman.  Increasing the Complexity of Coactivation in Nuclear Receptor Signaling , 1999, Cell.

[11]  G. Almouzni,et al.  Global Transcription Regulators of Eukaryotes , 1999, Cell.

[12]  K. Miyazono,et al.  Convergence of transforming growth factor-beta and vitamin D signaling pathways on SMAD transcriptional coactivators. , 1999, Science.

[13]  S. Kato,et al.  Selective Interaction of Vitamin D Receptor with Transcriptional Coactivators by a Vitamin D Analog , 1999, Molecular and Cellular Biology.

[14]  Chi V. Dang,et al.  c-Myc Target Genes Involved in Cell Growth, Apoptosis, and Metabolism , 1999, Molecular and Cellular Biology.

[15]  J. Qin,et al.  A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation. , 1999, Molecular cell.

[16]  David A. Agard,et al.  The Structural Basis of Estrogen Receptor/Coactivator Recognition and the Antagonism of This Interaction by Tamoxifen , 1998, Cell.

[17]  A. Eisen,et al.  Unraveling the role of helicases in transcription , 1998, BioEssays : news and reviews in molecular, cellular and developmental biology.

[18]  A. Lamond,et al.  Spliceosome assembly: The unwinding role of DEAD-box proteins , 1998, Current Biology.

[19]  A. Shyu,et al.  RNA stabilization by the AU‐rich element binding protein, HuR, an ELAV protein , 1998, The EMBO journal.

[20]  H. Erdjument-Bromage,et al.  A novel protein complex that interacts with the vitamin D3 receptor in a ligand-dependent manner and enhances VDR transactivation in a cell-free system. , 1998, Genes & development.

[21]  S. Shuman,et al.  The Nucleoside Triphosphatase and Helicase Activities of Vaccinia Virus NPH-II Are Essential for Virus Replication , 1998, Journal of Virology.

[22]  H. Gronemeyer,et al.  The coactivator TIF2 contains three nuclear receptor‐binding motifs and mediates transactivation through CBP binding‐dependent and ‐independent pathways , 1998, The EMBO journal.

[23]  U. Stahl,et al.  The protein family of RNA helicases. , 1998, Critical reviews in biochemistry and molecular biology.

[24]  E. Kalkhoven,et al.  Isoforms of steroid receptor co‐activator 1 differ in their ability to potentiate transcription by the oestrogen receptor , 1998, The EMBO journal.

[25]  S. Kato,et al.  25-Hydroxyvitamin D3 1α-Hydroxylase and Vitamin D Synthesis , 1997 .

[26]  P. Meltzer,et al.  AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer. , 1997, Science.

[27]  R. Evans,et al.  Nuclear Receptor Coactivator ACTR Is a Novel Histone Acetyltransferase and Forms a Multimeric Activation Complex with P/CAF and CBP/p300 , 1997, Cell.

[28]  Christopher K. Glass,et al.  The transcriptional co-activator p/CIP binds CBP and mediates nuclear-receptor function , 1997, Nature.

[29]  F. Fuller-Pace,et al.  p72: a human nuclear DEAD box protein highly related to p68. , 1996, Nucleic acids research.

[30]  P. Chambon.  A decade of molecular biology of retinoic acid receptors , 1996, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[31]  P. Chambon,et al.  TIF2, a 160 kDa transcriptional mediator for the ligand‐dependent activation function AF‐2 of nuclear receptors. , 1996, The EMBO journal.

[32]  Thorsten Heinzel,et al.  A CBP Integrator Complex Mediates Transcriptional Activation and AP-1 Inhibition by Nuclear Receptors , 1996, Cell.

[33]  K. Umesono,et al.  The nuclear receptor superfamily: The second decade , 1995, Cell.

[34]  Miguel Beato,et al.  Steroid hormone receptors: Many Actors in search of a plot , 1995, Cell.

[35]  Daniel Metzger,et al.  Activation of the Estrogen Receptor Through Phosphorylation by Mitogen-Activated Protein Kinase , 1995, Science.

[36]  B. O’Malley,et al.  Sequence and Characterization of a Coactivator for the Steroid Hormone Receptor Superfamily , 1995, Science.

[37]  P. Chambon,et al.  Widely spaced, directly repeated PuGGTCA elements act as promiscuous enhancers for different classes of nuclear receptors , 1995, Molecular and cellular biology.

[38]  T. Eki,et al.  Molecular cloning of cDNA encoding human DNA helicase Q1 which has homology to Escherichia coli Rec Q helicase and localization of the gene at chromosome 12p12. , 1994, Nucleic acids research.

[39]  M. Parker,et al.  Interaction of proteins with transcriptionally active estrogen receptors. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[40]  N. Webster,et al.  The human estrogen receptor has two independent nonacidic transcriptional activation functions , 1989, Cell.

[41]  J. Malter,et al.  Identification of an AUUUA-specific messenger RNA binding protein. , 1989, Science.

[42]  S. Fawell,et al.  Identification of two transactivation domains in the mouse oestrogen receptor. , 1989, Nucleic acids research.