Houseflies were reared on an artificial medium and tested with different wave-lengths of spectral light obtained from a quartz-mercury arc. The spectrum tested extended from λ3022 A to λ5780 A, and the lines were made of approximately equal intensity throughout. In addition, λ5461 A and λ4078 A were tested at several other intensities. The comparison standard in all cases was white light, obtained from a tungsten-filament, inside-frosted bulb, and filtered through copper sulphate solution. It was of constant quality, and the intensity was varied by changing the size of the bulb and by varying the distance from the bulb to the copper sulphate filter. The lighted areas to which the flies reacted were 5 by 10 mm. On one of these fell a total intensity of colored light of approximately 10.3 microwatts, on the other a range of intensity of white light of from 0.34 to 36.1 μw.Flies to be tested were removed from the breeding cage ten hours before tests began and were kept in darkness until used. Each fly whose ...
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