Specific Detection of Superoxide Ion Using Lucigenin Chemiluminescence in Mixed Vesicles Formed from Aqueous Mixtures of Octyltrimethylammonium Chloride and Sodium Dodecanoate

塩化オクチルトリメチルアンモニウム（OTAC）とドデカン酸ナトリウム（SD）の混合溶液中で形成される混合界面活性剤分子集合体（混合ベシクル）を，ルシゲニン化学発光（CL）の反応場として利用すると，活性酸素（スーパーオキシドO2−·，一重項酸素1O2，過酸化水素H2O2，ヒドロキシラジカル·OH）の中でO2−·が特異的，かつ高感度に検出できることを見いだした．5.0 M OTACと1.0 M SDの等量混合溶液（pH 7.0）中での活性酸素のCL応答はO2−·を100とすると1O2 0.011，H2O2 0.021，·OH 0.037で，O2−·の検出下限（3 σ）はヒポキサンチン濃度で10−7 Mレベルであった．混合ベシクル溶液中ではルシゲニン-O2−·反応の経路が非CL反応からCL反応へと劇的に変化し，著しくCL量子収率が向上したことによると考察した．本法をO2−·の生成が推定されている新規CL反応系に適用し，その有効性を示した．

[1]  Toshihiro Nakamura,et al.  Uranine Chemiluminescence in Aggregates Formed from Aqueous Mixtures of Dodecyltrimethylammonium Chloride and Sodium Dodecyl Sulfate , 2005 .

[2]  K. Ohno,et al.  Lucigenin Chemiluminescence in Mixed-Surfactant Vesicles Formed from Aqueous Mixtures of Octyltrimethylammonium Chloride and Sodium Dodecanoate , 2005 .

[3]  Jin‐Ming Lin,et al.  Microheterogeneous systems of micelles and microemulsions as reaction media in chemiluminescent analysis , 2003 .

[4]  J. Lin,et al.  Luminol chemiluminescence in unbuffered solutions with a cobalt(II)-ethanolamine complex immobilized on resin as catalyst and its application to analysis. , 2001, Analytical chemistry.

[5]  D. Richardson,et al.  Equilibria, Kinetics, and Mechanism in the Bicarbonate Activation of Hydrogen Peroxide: Oxidation of Sulfides by Peroxymonocarbonate , 2000 .

[6]  H. Zhu,et al.  Biochemical characterization of lucigenin (Bis-N-methylacridinium) as a chemiluminescent probe for detecting intramitochondrial superoxide anion radical production. , 1999, Biochemical and biophysical research communications.

[7]  J. Lin,et al.  Oxidation Reaction between Periodate and Polyhydroxyl Compounds and Its Application to Chemiluminescence. , 1999, Analytical chemistry.

[8]  Jin‐Ming Lin,et al.  Luminol-free chlorine chemiluminescence in an oil-in-water microemulsion medium , 1998 .

[9]  Jianbin Huang,et al.  Formation and coexistence of the micelles and vesicles in mixed solution of cationic and anionic surfactant , 1995 .

[10]  E. Ferber,et al.  Detergent-amplified chemiluminescence of lucigenin for determination of superoxide anion production by NADPH oxidase and xanthine oxidase. , 1988, Analytical biochemistry.

[11]  A. Campbell.  Chemiluminescence : principles and applications in biology and medicine , 1988 .

[12]  F. McCapra,et al.  Chemiluminescence in organic chemistry , 1987 .