Some haematological changes in hybrid catfish ( Heterobranchus longifilis x Clarias gariepinus ) fed different dietary levels of raw and boiled jackbean ( Canavalia ensiformis ) seed meal

Changes were observed in some haematological parameters of juvenile hybrid catfish (Heterobranchus longifilis x Clarias gariepinus) fed raw and 60 min-boiled jackbean seed meal (JBSM) at different dietary levels for 56 days. The haematocrit (packed cell volume, PCV), red blood cell (RBC) count, white blood cell (WBC) count and haemoglobin (Hb) concentration decreased significantly (P<0.05) with increasing dietary JBSM level. Though the mean values of the blood parameters of fish fed diets containing boiled JBSM (PCV = 29.93%; RBC = 1.2 x 106 mm-3; WBC = 15.91 x 103 mm-3; Hb = 8.31 g/100 ml) showed significant improvement when compared with those fed raw JBSM diets (PCV = 28.68%; RBC = 1.13 x 106 mm-3; WBC = 14.63 x 103 mm-3; Hb = 8.31 g/100 ml) they were, however, lower and significantly (P<0.05) different from those fed the control diet (PCV = 35.50%; RBC = 1.43 x 106 mm-3; WBC = 20.42 x 103 mm-3; Hb = 10.62 g/100 ml). However the observed reduction of the blood parameters did not go below the normal range of values recorded for catfish. Further investigation to improve the quality of boiled JBSM is suggested.

[1]  R. Ball,et al.  Influence of Capture Methods on Blood Characteristics and Mortality in the Rainbow Trout (Salmo gairdneri) , 1966 .

[2]  A. Udedibie.  Nutritional evaluation of jackbean (Canavalia ensiformis) for the Nigerian poultry industry , 1990 .

[3]  A. Tacon.  Nutritional fish pathology. Morphological signs of nutrient deficiency and toxicity in farmed fish , 1995 .

[4]  Susan Budavari,et al.  The Merck index : an encyclopedia of chemicals, drugs, and biologicals , 1983 .

[5]  R. C. Klinger,et al.  Effects of dietary lipid on the hematology of channel catfish, Ictalurus punctatus , 1996 .

[6]  Sidney A. Williams,et al.  Official Methods of Analysis of the Association of Official Analytical Chemists , 1971, Soil Science Society of America Journal.

[7]  C. Carlini,et al.  Questions and answers to edibility problem of the Canavalia ensiformis seeds – A review , 1998 .

[8]  E. Omoregie,et al.  Haematological Changes in the Mudfish, Clarias gariepinus (Burchell) Exposed to Malachite Green , 1999 .

[9]  C. Nnubia,et al.  Haematological studies on four catfish species raised in freshwater ponds in Nigeria , 1993 .

[10]  G. Wedemeyer,et al.  Clinical methods for the assessment of the effects of environmental stress on fish health , 1977 .

[11]  D. Whitmore,et al.  Considerations of blood parameters of largemouth bass micropterus salmoides , 1979 .

[12]  T. Morris,et al.  Effects of the inclusion of treated jack beans (Canavalia ensiformis) and the amino acid canavanine in chick diets , 1994, The Journal of Agricultural Science.

[13]  A. Ulrich,et al.  2. Analytical methods for use in plant analysis. , 1959 .

[14]  E. Wagner,et al.  Effects of Rearing Density upon Cutthroat Trout Hematology, Hatchery Performance, Fin Erosion, and General Health and Condition , 1997 .

[15]  T. Morris,et al.  Effects of raw and treated jack beans(Canavalia ensiformis) and of canavanine on the short-term feed intake of chicks and pigs , 1994, The Journal of Agricultural Science.

[16]  G. Rosenthal.  The Biological Effects and Mode of Action of L-Canavanine, a Structural Analogue of L-Arginine , 1977, The Quarterly Review of Biology.

[17]  R. A. Barnhart.  Effects of Certain Variables on Hematological Characteristics of Rainbow Trout , 1969 .

[18]  R. Bressani,et al.  Chemical composition, amino acid content and protein quality of Canavalia spp. seeds , 1987 .

[19]  P. C. Blaxhall,et al.  Routine haematological methods for use with fish blood , 1973 .

[20]  Fagbenro.  Use of full‐fat winged bean Psophocarpus tetragonolobus seed meal as a protein feedstuff in fish‐meal free diets for African catfish Clarias gariepinus , 1999 .

[21]  S. Mokady,et al.  Effects of soybean processing methods on the growth of carp (Cyprinus carpio) , 1983 .

[22]  M. Roberts,et al.  Some blood parameters of the rainbow trout (Salmo gairdneri Richardson) I. The Kamloops variety , 1973 .

[23]  H. Mooney,et al.  Effect of aquaculture on world fish supplies , 2000, Nature.

[24]  S. Clark,et al.  Whitemore and Consideration of blood parameters of largemouth bass Micropterus salmoides J. , 1979 .

[25]  D. Davis,et al.  Partial Replacement of Fish Meal with Meat-and-Bone Meal, Flash-Dried Poultry By-Product Meal, and Enzyme-Digested Poultry By-Product Meal in Practical Diets for Juvenile Red Drum , 2000 .

[26]  P. Cheeke.  NUTRITIONAL AND PHYSIOLOGICAL IMPLICATIONS OF SAPONINS: A REVIEW , 1971 .

[27]  J. Hattingh,et al.  The influence of capture and transportation on some blood parameters of fresh water fish. , 1974, Comparative biochemistry and physiology. A, Comparative physiology.

[28]  A. Ologhobo,et al.  Utilisation of raw jackbean (Canavalia ensiformis) and jackbean fractions in diets for broiler chicks , 1993 .

[29]  Kenneth Helrich,et al.  Official methods of analysis of the Association of Official Analytical Chemists , 1990 .

[30]  R. Roberts,et al.  The hepato renal syndrome of cultured turbot its effect on a 2 year trial of farm reared turbot scophthalmus maximus , 1976 .

[31]  E. Allison.  Big laws, small catches: global ocean governance and the fisheries crisis , 2001 .