Cell proliferation in nasal respiratory epithelium of people exposed to urban pollution.

The nasal passages are a common portal of entry and are a prime site for toxicant-induced pathology. Sustained increases in regenerative cell proliferation can be a significant driving force in chemical carcinogenesis. The atmosphere in Mexico City contains a complex mixture of air pollutants and its residents are exposed chronically and sequentially to numerous toxicants and potential carcinogens. We were concerned that exposure to Mexico City's atmosphere might induce cytotoxicity and increase nasal respiratory epithelial cell proliferation. Nasal biopsies were obtained for DNA cell cycle analysis from 195 volunteers. The control population consisted of 16 adults and 27 children that were residents in a Caribbean island with low pollution. The exposed Mexico City population consisted of 109 adults and 43 children. Sixty-one of the adult subjects were newly arrived in Mexico City and were followed for 25 days from their arrival. Control children, control adult and exposed Mexico City children all had similar percentages of cells in the replicative DNA synthesis phase (S phase) of the cell cycle (%S). A significant increase in %S in nasal epithelial cells was seen in exposed adult residents in Mexico City biopsied at three different dates compared with control adults. Newly arrived adults exhibited a control level of cell turnover at day 2 after coming to the city. However, at days 7, 14 and 25 they exhibited significant increases in %S. These data demonstrate an increased and sustained nasal cell turnover rate in the adult population observable in as little as 1 week of residence in Mexico City. This increase in cell proliferation is in agreement with other reports of induced pathological changes in the nasal passages of Mexico City dwellers. These observations suggest an increased potential risk factor of developing nasal neoplasms for residents of large cities with heavy pollution.

[1]  V. Feron,et al.  Carcinogenic response of the nasal cavity to inhaled chemical mixtures. , 1997, Mutation research.

[2]  B. Butterworth.  Chemically induced cell proliferation. Implications for risk assessment. Proceedings of the Chemically Induced Cell Proliferation Conference. Austin, Texas, November 29-December 2, 1989. , 1991, Progress in clinical and biological research.

[3]  V. Feron,et al.  Nasal tumours in rats after severe injury to the nasal mucosa and prolonged exposure to 10 ppm formaldehyde , 1989, Journal of applied toxicology : JAT.

[4]  R B Conolly,et al.  A strategy for establishing mode of action of chemical carcinogens as a guide for approaches to risk assessments. , 1995, Cancer letters.

[5]  H. Koren,et al.  Biomarkers of inflammation in ozone-exposed humans. Comparison of the nasal and bronchoalveolar lavage. , 1990, The American review of respiratory disease.

[6]  M. Bogdanffy,et al.  Effects of propylene oxide on nasal epithelial cell proliferation in F344 rats. , 1995, Fundamental and applied toxicology : official journal of the Society of Toxicology.

[7]  K. Morgan,et al.  Chemically-induced nasal carcinogenesis and epithelial cell proliferation: a brief review. , 1997, Mutation research.

[8]  Kevin T. Morgan,et al.  Review Article: A Brief Review of Formaldehyde Carcinogenesis in Relation to Rat Nasal Pathology and Human Health Risk Assessment , 1997 .

[9]  L. Calderón-Garcidueñas,et al.  DNA strand breaks in human nasal respiratory epithelium are induced upon exposure to urban pollution. , 1996, Environmental health perspectives.

[10]  F. Sundler,et al.  Contribution of Plasma‐Derived Molecules to Mucosal Immune Defence, Disease and Repair in the Airways , 1998, Scandinavian journal of immunology.

[11]  R B Conolly,et al.  Chemically induced cell proliferation in carcinogenesis. , 1992, IARC scientific publications.

[12]  R. Henderson,et al.  Proliferative responses of rat nasal epithelia to ozone. , 1990, Toxicology and applied pharmacology.

[13]  J. Zahm,et al.  Epithelial barrier integrity during in vitro wound repair of the airway epithelium. , 1996, American journal of respiratory cell and molecular biology.

[14]  S. Takenaka,et al.  Chronic effects on the respiratory tract of hamsters, mice and rats after long‐term inhalation of high concentrations of filtered and unfiltered diesel engine emissions , 1986, Journal of applied toxicology : JAT.

[15]  G. Oberdörster,et al.  Carcinogenicity assessment of selected nickel compounds. , 1997, Toxicology and applied pharmacology.

[16]  A. Bravo,et al.  Analysis of the change in atmospheric urban formaldehyde and photochemistry activity as a result of using methyl-t-butyl-ether (MTBE) as an additive in gasolines of the metropolitan area of Mexico city , 1991 .

[17]  D. Blake,et al.  Urban Leakage of Liquefied Petroleum Gas and Its Impact on Mexico City Air Quality , 1995, Science.

[18]  E. H. Vernot,et al.  Oncogenic potential of inhaled hydrazine in the nose of rats and hamsters after 1 or 10 1-hr exposures. , 1995, Fundamental and applied toxicology : official journal of the Society of Toxicology.

[19]  K. Salnikow,et al.  Molecular Mechanisms of Nickel Carcinogenesis , 1995, Biological chemistry.

[20]  L. Calderón-Garcidueñas,et al.  Nasal inflammatory responses in children exposed to a polluted urban atmosphere. , 1995, Journal of toxicology and environmental health.

[21]  V. Feron,et al.  Nasal tumours in rats after short-term exposure to a cytotoxic concentration of formaldehyde. , 1988, Cancer letters.

[22]  O R Moss,et al.  DNA-protein cross-links and cell replication at specific sites in the nose of F344 rats exposed subchronically to formaldehyde. , 1994, Fundamental and applied toxicology : official journal of the Society of Toxicology.

[23]  P. Borm,et al.  Neutrophils amplify the formation of DNA adducts by benzo[a]pyrene in lung target cells. , 1997, Environmental health perspectives.

[24]  L. Calderón-Garcidueñas,et al.  Nasal cytology in southwest metropolitan Mexico City inhabitants: a pilot intervention study. , 1993, Environmental health perspectives.

[25]  K. Morgan,et al.  Cell proliferation and formaldehyde-induced respiratory carcinogenesis. , 1994, Risk analysis : an official publication of the Society for Risk Analysis.

[26]  J Cherry,et al.  The Kinetics of Cellular Proliferation in Normal and Malignant Tissues X. Cell Proliferation in the Nose and Adjoining Cavities , 1970, The Annals of otology, rhinology, and laryngology.

[27]  K. Nikula,et al.  The response of the rat tracheal epithelium to ozone exposure. Injury, adaptation, and repair. , 1988, The American journal of pathology.

[28]  D. Hyde,et al.  Response of the macaque nasal epithelium to ambient levels of ozone. A morphologic and morphometric study of the transitional and respiratory epithelium. , 1987, The American journal of pathology.

[29]  A. Báez,et al.  Measurements of formaldehyde and acetaldehyde in the atmosphere of Mexico City. , 1995, Environmental pollution.

[30]  J. Resau,et al.  Formaldehyde-Induced Lesions of Xenotransplanted Human Nasal Respiratory Epithelium , 1989, Toxicologic pathology.

[31]  R. Kiss,et al.  Determination of proliferative activity in nasal polyps. , 1997, Journal of Clinical Pathology.

[32]  H. Hansson,et al.  Regenerating human nasal mucosa cells express peptide growth factors. , 1991, Archives of otolaryngology--head & neck surgery.

[33]  K T Morgan,et al.  Effects of formaldehyde gas on the respiratory tract of rhesus monkeys. Pathology and cell proliferation. , 1989, The American journal of pathology.

[34]  J A Swenberg,et al.  Carcinogenicity of formaldehyde in rats and mice after long-term inhalation exposure. , 1983, Cancer research.

[35]  H. Vainio.  Mechanisms of Carcinogenesis in Risk Identification , 1992 .

[36]  K T Morgan,et al.  A brief review of formaldehyde carcinogenesis in relation to rat nasal pathology and human health risk assessment. , 1997, Toxicologic pathology.

[37]  R. McClellan,et al.  Response of rodents to inhaled diluted diesel exhaust: biochemical and cytological changes in bronchoalveolar lavage fluid and in lung tissue. , 1988, Fundamental and applied toxicology : official journal of the Society of Toxicology.

[38]  S. Gómez-Arroyo,et al.  Mutagenicity assessment of airborne particles in Mexico City , 1995 .

[39]  E. Escudier,et al.  Epithelial Cell Proliferation, Apoptosis, and Apoptosis Inhibition in Inverted Papillomas , 1998, The Laryngoscope.

[40]  W. Panje,et al.  Proliferation of epithelia of noninvolved mucosa in patients with head and neck cancer , 1996, Head & neck.

[41]  V. Feron,et al.  Subchronic (13-week) inhalation toxicity study of formaldehyde in male rats: 8-hour intermittent versus 8-hour continuous exposures. , 1989, Toxicology letters.

[42]  J. Harkema,et al.  Endotoxin or cytokines attenuate ozone-induced DNA synthesis in rat nasal transitional epithelium. , 1992, Toxicology and applied pharmacology.

[43]  H. Koren,et al.  Nasal lavage as a tool in assessing acute inflammation in response to inhaled pollutants. , 1990, Toxicology.

[44]  F. Fry,et al.  Regional deposition and clearance of particles in the human nose , 1973 .

[45]  H. Witschi,et al.  Cumulative labeling indices in epithelial cell populations of the respiratory tract after exposure to ozone at low concentrations. , 1995, Toxicology and applied pharmacology.

[46]  R. Albert,et al.  Issues in the risk assessment of chromium. , 1991, Environmental health perspectives.

[47]  L. Calderón-Garcidueñas,et al.  Histopathologic changes of the nasal mucosa in southwest Metropolitan Mexico City inhabitants. , 1992, The American journal of pathology.

[48]  K. Kolaja,et al.  The role of oxidative stress in chemical carcinogenesis. , 1998, Environmental health perspectives.

[49]  V. Feron,et al.  Interactive effects of ozone and formaldehyde on the nasal respiratory lining epithelium in rats. , 1990, Journal of toxicology and environmental health.

[50]  J M Zahm,et al.  Cell migration and proliferation during the in vitro wound repair of the respiratory epithelium. , 1997, Cell motility and the cytoskeleton.

[51]  L. Calderón-Garcidueñas,et al.  DNA damage in nasal respiratory epithelium from children exposed to urban pollution , 1997, Environmental and molecular mutagenesis.

[52]  F. Hahn,et al.  Comparative carcinogenic effects of nickel subsulfide, nickel oxide, or nickel sulfate hexahydrate chronic exposures in the lung. , 1995, Cancer research.

[53]  R. Henderson,et al.  Effect of cumulative ozone exposure on ozone-induced nasal epithelial hyperplasia and secretory metaplasia in rats. , 1991, Experimental lung research.

[54]  J A Swenberg,et al.  Correlation of regional and nonlinear formaldehyde-induced nasal cancer with proliferating populations of cells. , 1996, Cancer research.

[55]  R M Fay,et al.  Complex mixtures: hazard identification and risk assessment. , 1996, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[56]  James A. Swenberg.  Cell Proliferation and Chemical Carcinogenesis: Conference Summary and Future Directions , 1993 .

[57]  N. Johnson,et al.  Kinetics of nasal epithelial cell loss and proliferation in F344 rats following a single exposure to 0.5 ppm ozone. , 1997, Toxicology and applied pharmacology.

[58]  C. Barrow,et al.  Nasal cavity deposition, histopathology, and cell proliferation after single or repeated formaldehyde exposures in B6C3F1 mice and F-344 rats. , 1983, Toxicology and applied pharmacology.

[59]  C. Cross,et al.  ANTIOXIDANT PROTECTION: A FUNCTION OF TRACHEOBRONCHIAL AND GASTROINTESTINAL MUCUS , 1984, The Lancet.

[60]  K. B. Gross,et al.  Pulmonary functional and morphological changes induced by a 4-week exposure to 0.7 ppm ozone followed by a 9-week recovery period. , 1986, Journal of toxicology and environmental health.