Three SRAP Molecular Markers Linked to Yield Component Traits in Wheat

In order to better understand the relationship genotype-phenotype, marker-trait associations were studied in eight wheat genotypes using a set of 95 DNA-based SRAP molecular markers. SRAP analysis showed that the average of the percentage of polymorphism (P%) was 62.59%, as well as the average of polymorphic information content (PIC) was 0.23. Moreover, the means of marker index (MI) was 1.49. The ME1F-EM5R and ME9F-EM3R primers combination showed higher levels of polymorphism of 88.89% and 90.91%, respectively. Single-marker analysis (SMA) indicated that ME-7F-EM-6R1250bp was probably as a candidate marker which linked to 1000-grain weight. In addition, ME-7F-EM-5R900bp and ME-4F-EM-6R490bp SRAP markers were identified for weight of grains/spike and No. of grains/spike, respectively. The cluster analysis based on SRAP and means of morphological data revealed similarity coefficient values ranged from 57.40% to 80.40% and from 90.61% to 98.81%, respectively. Similarity matrices generated by SRAP markers and means of morphological traits showed a positive but non-significant correlation (r = 0.03, P˃ 0.05).