Comparative Screening Analytic Methods for Elderly of Blood Methylmercury Concentration between Two Analytical Institutions

Methylmercury is widely known to be a toxic substance in the human, especially a nervous system. However, it is difficult to accurately measure the amount of methylmercury in blood, and the form of methylmercury is variously presented. The purpose of study was to compare the total mercury and methylmercury measurements techniques and detection levels between analytical institutions in two countries using the same elderly human blood samples. Total mercury using gold amalgamation direct mercury analysis method (both) and methylmercury using the dithizone extraction and gas chromatography-electron capture detector (GC-ECD) method (N Lab in Japan) and the cold vapor atomic fluorescence spectrophotometer (CVAFS) method (D Lab in Korea) were measured in 47 subjects who agreed to participate in this study. Total mercury concentrations in both analytical laboratories were observed at similar levels (9.4 versus 9.5 ug/kg, p=0.898) and the distribution was highly correlated. However, the concentration of methylmercury showed some difference between two laboratories (9.1 versus 8.6 ug/kg, p<0.001). Due to different recovery rates by different analytical methods, it is assumed that the methyl/total mercury ratio in N lab in Japan was higher than D lab in Korea (96.8 versus 90.4%, p<0.001). The GC-ECD was more sensitive method than CVAFS in methylmercury analytic techniques.

[1]  Fish consumption, mercury exposure, and heart diseases. , 2004, Nutrition reviews.

[2]  J. Ruedy,et al.  Methyl mercury exposure in northern Quebec. II. Neurologic findings in children. , 1983, American journal of epidemiology.

[3]  Margaret R. Karagas,et al.  Evidence on the Human Health Effects of Low-Level Methylmercury Exposure , 2012, Environmental health perspectives.

[4]  M. Harada,et al.  Minamata disease: methylmercury poisoning in Japan caused by environmental pollution. , 1995, Critical reviews in toxicology.

[5]  P. Grandjean,et al.  Adverse Effects of Methylmercury: Environmental Health Research Implications , 2010, Environmental health perspectives.

[6]  Megumi Yamamoto,et al.  Methylmercury analyses in biological materials by heating vaporization atomic absorption spectrometry. , 2010, The Journal of toxicological sciences.

[7]  Megumi Yamamoto,et al.  Simple analysis of total mercury and methylmercury in seafood using heating vaporization atomic absorption spectrometry. , 2016, The Journal of toxicological sciences.

[8]  Tracey J. Woodruff,et al.  Dose–Response Relationship of Prenatal Mercury Exposure and IQ: An Integrative Analysis of Epidemiologic Data , 2007, Environmental health perspectives.

[9]  G. Nordberg,et al.  A review of normal concentrations of mercury in human blood. , 1991, The Science of the total environment.

[10]  Y. Takizawa,et al.  Human exposure to mercury due to goldmining in the Tapajos River basin, Amazon, Brazil: Speciation of mercury in human hair, blood and urine , 1995 .

[11]  Kathleen J. Mullen,et al.  Agricultural Policies in India , 2018, OECD Food and Agricultural Reviews.

[12]  J. C. Smith,et al.  Gas-chromatographic determination of inorganic mercury and organomercurials in biological materials. , 1977, Analytical chemistry.

[13]  Byung-Seong Suh,et al.  Analysis of Methylmercury Concentration in the Blood of Koreans by Using Cold Vapor Atomic Fluorescence Spectrophotometry , 2011, Annals of laboratory medicine.

[14]  Byung-Kook Lee,et al.  Blood total mercury and fish consumption in the Korean general population in KNHANES III, 2005. , 2010, The Science of the total environment.

[15]  B. H. Choi,et al.  Oxidative stress in neurotoxic effects of methylmercury poisoning. , 1996, Neurotoxicology.

[16]  C Cox,et al.  Effects of prenatal and postnatal methylmercury exposure from fish consumption on neurodevelopment: outcomes at 66 months of age in the Seychelles Child Development Study. , 1998, JAMA.

[17]  J. Hammitt,et al.  A probabilistic characterization of the health benefits of reducing methyl mercury intake in the United States. , 2010, Environmental science & technology.

[18]  T. Clarkson,et al.  Metal toxicity in the central nervous system. , 1987, Environmental health perspectives.

[19]  Robert L. Jones,et al.  Total and methyl mercury in whole blood measured for the first time in the U.S. population: NHANES 2011-2012. , 2014, Environmental research.